Surface Profiling of Extracellular Vesicles from Plasma or Ascites Fluid Using DotScan Antibody Microarrays

2017 ◽  
pp. 263-301 ◽  
Author(s):  
Larissa Belov ◽  
Susannah Hallal ◽  
Kieran Matic ◽  
Jerry Zhou ◽  
Sandra Wissmueller ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Ascites Fluid ◽  
Antibody Microarrays ◽  
Surface Profiling

Advantageous Antibody Microarray Fabrication Through DNA-Directed Immobilization: A Step Toward Use of Extracellular Vesicles in Diagnostics

2020 ◽  
Author(s):  
Dario Brambilla ◽  
Laura Sola ◽  
Marcella Chiari
Keyword(s):  
Extracellular Vesicles ◽  
Dna Arrays ◽  
Antibody Microarray ◽  
Antibody Microarrays ◽  
Protein Ligation ◽  
Microarray Fabrication

An optimized general protocol for DNA-protein ligation is provided and the conjugates are used to convert DNA arrays into antibody microarrays. Arrays obtained through DDI were used to capture and characterize extracellular vesicles (EVs), an emerging class of biomarkers. The proposed platform was tested against commercially available antibody microarrays, showing good performance combined with ease of fabrication.

Extracellular Vesicles in Liver Diseases: Diagnostic, Prognostic, and Therapeutic Application

2019 ◽  
Vol 39 (01) ◽  
pp. 070-077 ◽  
Author(s):  
Sabine Urban ◽  
Tudor Mocan ◽  
Hanna Sänger ◽  
Veronika Lukacs-Kornek ◽  
Miroslaw Kornek
Keyword(s):  
Extracellular Vesicles ◽  
Liver Diseases ◽  
Screening Tool ◽  
Liver Disorder ◽  
Disease Diagnosis ◽  
Extracellular Vesicle ◽  
Nonalcoholic Fatty Liver ◽  
Surface Profiling ◽  
Diagnosis And Prognosis ◽  
Disease Diagnostics

AbstractExtracellular vesicles, comprising exosomes, microvesicles, and apoptotic bodies, represent an emerging field in disease diagnostics and prognosis. They can be isolated from peripheral blood of patients as well as from other body fluids and can therefore be considered a minimally invasive liquid biopsy screening tool. Especially their surface antigen composition can reveal information about disease backgrounds. For several liver diseases, including fatal hepatocellular and cholangiocellular carcinoma as well as other nonmalignant liver disorders such as nonalcoholic fatty liver disease, alcoholic hepatitis, or acute liver failure, it has been shown that extracellular vesicle (EV) surface profiling can be useful for disease diagnosis and prognosis. This review focuses on latest advances in these areas to improve liver disorder detection and management. Additionally, the authors will discuss possible therapeutic applications of EVs in liver diseases, which might be a potent treatment option in the future.

Advantageous Antibody Microarray Fabrication Through DNA-Directed Immobilization: A Step Toward Use of Extracellular Vesicles in Diagnostics

2020 ◽  
Author(s):  
Dario Brambilla ◽  
Laura Sola ◽  
Marcella Chiari
Keyword(s):  
Extracellular Vesicles ◽  
Dna Arrays ◽  
Antibody Microarray ◽  
Antibody Microarrays ◽  
Protein Ligation ◽  
Microarray Fabrication

An optimized general protocol for DNA-protein ligation is provided and the conjugates are used to convert DNA arrays into antibody microarrays. Arrays obtained through DDI were used to capture and characterize extracellular vesicles (EVs), an emerging class of biomarkers. The proposed platform was tested against commercially available antibody microarrays, showing good performance combined with ease of fabrication.

Experiments with a scanning tunneling microscope (STM) mounted in a scanning electron microscope (SEM)

1986 ◽  
Vol 44 ◽  
pp. 636-639
Author(s):  
Oliver C. Wells ◽  
Mark E. Welland
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Mechanical Stability ◽  
Tunneling Current ◽  
Feedback System ◽  
Scanning Tunneling ◽  
Surface Profiling ◽  
Close Proximity ◽  
Metal Tip ◽  
Scanning Electron

Scanning tunneling microscopes (STM) exist in two versions. In both of these, a pointed metal tip is scanned in close proximity to the specimen surface by means of three piezos. The distance of the tip from the sample is controlled by a feedback system to give a constant tunneling current between the tip and the sample. In the low-end STM, the system has a mechanical stability and a noise level to give a vertical resolution of between 0.1 nm and 1.0 nm. The atomic resolution STM can show individual atoms on the surface of the specimen.A low-end STM has been put into the specimen chamber of a scanning electron microscope (SEM). The first objective was to investigate technological problems such as surface profiling. The second objective was for exploratory studies. This second objective has already been achieved by showing that the STM can be used to study trapping sites in SiO2.

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