Molecular Identification of Date Palm Cultivars Using Random Amplified Polymorphic DNA (RAPD) Markers

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

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Phenetic relationship and identification of subtribe Oncidiinae genotypes by random amplified polymorphic DNA (RAPD) markers

Scientia Horticulturae ◽

10.1016/s0304-4238(02)00088-2 ◽

2002 ◽

Vol 96 (1-4) ◽

pp. 303-312 ◽

Cited By ~ 2

Author(s):

C.C Tsai ◽

S.C Huang ◽

P.L Huang ◽

Y.S Chen ◽

C.H Chou

Keyword(s):

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Phenetic Relationship

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Detection and analysis of genetic variation in Salicornieae (Chenopodiaceae) using random amplified polymorphic DNA (RAPD) markers

Taxon ◽

10.2307/1222677 ◽

1995 ◽

Vol 44 (1) ◽

pp. 53-63 ◽

Cited By ~ 13

Author(s):

T. Luque ◽

C. Ruiz ◽

J. Avalos ◽

I. L. Calderón ◽

M. E. Figueroa

Keyword(s):

Genetic Variation ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna

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DNA profiling of commercial chilli pepper (Capsicum annuum L.) varieties using random amplified polymorphic DNA (RAPD) markers

AFRICAN JOURNAL OF BIOTECHNOLOGY ◽

10.5897/ajb2012.3017 ◽

2013 ◽

Vol 12 (30) ◽

pp. 4730-4735 ◽

Cited By ~ 2

Author(s):

Prasad Bylla ◽

Gulab Khan R ◽

Radha T ◽

Ravi Ch ◽

Venkataiah P ◽

...

Keyword(s):

Capsicum Annuum ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Dna Profiling ◽

Chilli Pepper ◽

Capsicum Annuum L

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Genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm revealed by RAPD markers

Genome ◽

10.1139/g01-096 ◽

2001 ◽

Vol 44 (6) ◽

pp. 995-999 ◽

Cited By ~ 25

Author(s):

H I Amadou ◽

P J Bebeli ◽

P J Kaltsikes

Keyword(s):

Genetic Diversity ◽

Cluster Analysis ◽

Rapd Markers ◽

Random Amplified Polymorphic Dna ◽

Geographic Origin ◽

Bambara Groundnut ◽

International Institute ◽

Tropical Agriculture ◽

Vigna Subterranea ◽

Ibadan Nigeria

Random amplified polymorphic DNA (RAPD) markers were used to assess genetic diversity in Bambara groundnut (Vigna subterranea L.) germplasm using 25 African accessions from the collection in the International Institute for Tropical Agriculture, Ibadan, Nigeria. Fifty random decamer primers were screened to assess their ability to detect polymorphism in bambara; 17 of them were selected for this study. Considerable genetic diversity was found among the V. subterranea accessions studied. The relationships among the 25 accessions were studied by cluster analysis. The dendrograms showed two main groups of accessions mainly along the lines of their geographic origin. It is concluded that RAPD can be used for germplasm classification in bambara groundnut and hence for improving this crop.Key words: germplasm, PCR, RAPD, Vigna subterranea.

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Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers

Genome ◽

10.1139/g95-025 ◽

1995 ◽

Vol 38 (2) ◽

pp. 201-210 ◽

Cited By ~ 89

Author(s):

F. N. Wachira ◽

R. Waugh ◽

W. Powell ◽

C. A. Hackett

Keyword(s):

Genetic Diversity ◽

Genetic Variability ◽

Southeast Asia ◽

Camellia Sinensis ◽

Rapd Markers ◽

Rapd Analysis ◽

Random Amplified Polymorphic Dna ◽

Systematic Assessment ◽

Tree Crop ◽

Taxonomic Relationships

Camellia sinensis is a beverage tree crop native to Southeast Asia and introductions have been made into several nonindigenous countries. No systematic assessment of genetic variability in tea has been done anywhere. In this study, random amplified polymorphic DNA (RAPD) analysis was used to estimate genetic diversity and taxonomic relationships in 38 clones belonging to the three tea varieties, assamica, sinensis, and assamica ssp. lasiocalyx. Extensive genetic variability was detected between species, which was partitioned into between and within population components. Seventy percent of the variation was detected within populations. Analyses based on band sharing separated the three populations in a manner consistent with both the present taxonomy of tea and with the known pedigrees of some clones. RAPD analysis also discriminated all of the 38 commercial clones, even those which cannot be distinguished on the basis of morphological and phenotypic traits.Key words: genetic diversity, RAPDs, Camellia sinensis.

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