The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen

2018 ◽  
pp. 193-205 ◽  
Author(s):  
Chung Park ◽  
Il-Young Hwang ◽  
John H. Kehrl
Keyword(s):  
Lymph Nodes ◽  
B Lymphocyte ◽  
Confocal Imaging ◽  
Thick Section ◽  
Lymphocyte Trafficking ◽  
Two Photon

scholarly journals Lymph node B lymphocyte trafficking is constrained by anatomy and highly dependent upon chemoattractant desensitization

Blood ◽  
2012 ◽  
Vol 119 (4) ◽  
pp. 978-989 ◽  
Author(s):  
Chung Park ◽  
Il-Young Hwang ◽  
Rajesh K. Sinha ◽  
Olena Kamenyeva ◽  
Michael D. Davis ◽  
...  
Keyword(s):  
Cell Migration ◽  
Lymph Node ◽  
B Cells ◽  
Lymph Nodes ◽  
B Cell ◽  
B Lymphocyte ◽  
Lymphocyte Trafficking ◽  
High Endothelial Venules ◽  
Endothelial Barriers ◽  
Basal Portion

Abstract B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited the HEV perivenule space, and thereafter localized nearby, avoiding the follicle. Eventually, the newly arrived B cells entered the basal portion of the follicle gradually populating it. In contrast, newly arriving activated B cells rapidly crossed HEVs and migrated toward the lymph node follicle. During their LN residency, recirculating B cells reacquired their sphingosine-1 phospate receptor 1 (S1P1) receptors and markedly attenuated their sensitivity to chemokines. Eventually, the B cells exited the LN follicle by entering the cortical lymphatics or returning to the paracortical cords. Upon entering the lymph, the B cells lost their polarity, down-regulated their S1P1 receptors, and subsequently strongly up-regulated their sensitivity to chemokines. These results are summarized in a model of homeostatic trafficking of B cells through LNs.

Migratory cues controlling B‐lymphocyte trafficking in human lymph nodes

2020 ◽  
Vol 99 (1) ◽  
pp. 49-64
Author(s):  
Saem Mul Park ◽  
Anna ES Brooks ◽  
Chun‐Jen J Chen ◽  
Hilary M Sheppard ◽  
Evert Jan Loef ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
B Lymphocyte ◽  
Lymphocyte Trafficking

scholarly journals In vivo two-photon imaging of T cell motility in joint-draining lymph nodes in a mouse model of rheumatoid arthritis

2012 ◽  
Vol 278 (1-2) ◽  
pp. 158-165 ◽  
Author(s):  
Tamás Kobezda ◽  
Sheida Ghassemi-Nejad ◽  
Tibor T. Glant ◽  
Katalin Mikecz
Keyword(s):  
Rheumatoid Arthritis ◽  
T Cell ◽  
Mouse Model ◽  
Lymph Nodes ◽  
Cell Motility ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging ◽  
Draining Lymph Nodes

Two-photon confocal imaging study: Cell uptake of two photon dyes-labeled PAMAM dendrons in HeLa cells

2012 ◽  
Vol 100A (3) ◽  
pp. 746-756 ◽  
Author(s):  
Hsieh-Chih Tsai ◽  
Toyoko Imae ◽  
Gabriela Calderó ◽  
Conxita Solans
Keyword(s):  
Hela Cells ◽  
Imaging Study ◽  
Confocal Imaging ◽  
Cell Uptake ◽  
Two Photon

T and B Lymphocyte Populations in Uterus Draining Lymph Nodes at Estrus and Diestrus in Wistar Rats

1988 ◽  
Vol 16 (4) ◽  
pp. 143-145 ◽  
Author(s):  
LUCAS L. COLOMBO ◽  
MARÍA DEL C. LÓPEZ ◽  
JORGE HERKOVITS ◽  
MARÍA E. ROUX
Keyword(s):  
Lymph Nodes ◽  
Wistar Rats ◽  
B Lymphocyte ◽  
Lymphocyte Populations ◽  
Draining Lymph Nodes

Two-photon fluorescence properties of curcumin as a biocompatible marker for confocal imaging

2012 ◽  
Vol 100 (20) ◽  
pp. 203701 ◽  
Author(s):  
Abhishek Kumar ◽  
Lian Li ◽  
Akanksha Chaturvedi ◽  
Joseph Brzostowski ◽  
Joshna Chittigori ◽  
...  
Keyword(s):  
Confocal Imaging ◽  
Fluorescence Properties ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

scholarly journals Determining composition of micron-scale protein deposits in neurodegenerative disease by spatially targeted optical microproteomics

eLife ◽  
2015 ◽  
Vol 4 ◽  
Author(s):  
Kevin C Hadley ◽  
Rishi Rakhit ◽  
Hongbo Guo ◽  
Yulong Sun ◽  
James EN Jonkman ◽  
...  
Keyword(s):  
Fluorescent Dyes ◽  
Genetic Manipulation ◽  
Ex Vivo ◽  
Confocal Imaging ◽  
Test Case ◽  
Post Mortem ◽  
Two Photon ◽  
Protein Deposits ◽  
Photon Excitation ◽  
Formalin Fixed

Spatially targeted optical microproteomics (STOMP) is a novel proteomics technique for interrogating micron-scale regions of interest (ROIs) in mammalian tissue, with no requirement for genetic manipulation. Methanol or formalin-fixed specimens are stained with fluorescent dyes or antibodies to visualize ROIs, then soaked in solutions containing the photo-tag: 4-benzoylbenzyl-glycyl-hexahistidine. Confocal imaging along with two photon excitation are used to covalently couple photo-tags to all proteins within each ROI, to a resolution of 0.67 µm in the xy-plane and 1.48 µm axially. After tissue solubilization, photo-tagged proteins are isolated and identified by mass spectrometry. As a test case, we examined amyloid plaques in an Alzheimer's disease (AD) mouse model and a post-mortem AD case, confirming known plaque constituents and discovering new ones. STOMP can be applied to various biological samples including cell lines, primary cell cultures, ex vivo specimens, biopsy samples, and fixed post-mortem tissue.

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