Faculty Opinions recommendation of Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes.

Abstract B lymphocyte recirculation through lymph nodes (LNs) requires crossing endothelial barriers and chemoattractant-triggered cell migration. Here we show how LN anatomy and chemoattractant receptor signaling organize B lymphocyte LN trafficking. Blood-borne B cells predominately used CCR7 signaling to adhere to high endothelial venules (HEVs). New B cell emigrants slowly transited the HEV perivenule space, and thereafter localized nearby, avoiding the follicle. Eventually, the newly arrived B cells entered the basal portion of the follicle gradually populating it. In contrast, newly arriving activated B cells rapidly crossed HEVs and migrated toward the lymph node follicle. During their LN residency, recirculating B cells reacquired their sphingosine-1 phospate receptor 1 (S1P1) receptors and markedly attenuated their sensitivity to chemokines. Eventually, the B cells exited the LN follicle by entering the cortical lymphatics or returning to the paracortical cords. Upon entering the lymph, the B cells lost their polarity, down-regulated their S1P1 receptors, and subsequently strongly up-regulated their sensitivity to chemokines. These results are summarized in a model of homeostatic trafficking of B cells through LNs.

Download Full-text

Mst1 controls lymphocyte trafficking and interstitial motility within lymph nodes

The EMBO Journal ◽

10.1038/emboj.2009.82 ◽

2009 ◽

Vol 28 (9) ◽

pp. 1319-1331 ◽

Cited By ~ 119

Author(s):

Koko Katagiri ◽

Tomoya Katakai ◽

Yukihiko Ebisuno ◽

Yoshihiro Ueda ◽

Takaharu Okada ◽

...

Keyword(s):

Lymph Nodes ◽

Lymphocyte Trafficking

Download Full-text

Migratory cues controlling B‐lymphocyte trafficking in human lymph nodes

Immunology and Cell Biology ◽

10.1111/imcb.12386 ◽

2020 ◽

Vol 99 (1) ◽

pp. 49-64

Author(s):

Saem Mul Park ◽

Anna ES Brooks ◽

Chun‐Jen J Chen ◽

Hilary M Sheppard ◽

Evert Jan Loef ◽

...

Keyword(s):

Lymph Nodes ◽

B Lymphocyte ◽

Lymphocyte Trafficking

Download Full-text

The Role of Chemokines in Lymphocyte Trafﬁcking to Lymph Nodes of the Lung

Chemokines in the Lung ◽

10.1201/b14091-8 ◽

2003 ◽

pp. 72-89

Keyword(s):

Lymph Nodes ◽

Lymphocyte Trafficking

Download Full-text

The Use of Intravital Two-Photon and Thick Section Confocal Imaging to Analyze B Lymphocyte Trafficking in Lymph Nodes and Spleen

Methods in Molecular Biology - B Cell Receptor Signaling ◽

10.1007/978-1-4939-7474-0_14 ◽

2018 ◽

pp. 193-205 ◽

Cited By ~ 5

Author(s):

Chung Park ◽

Il-Young Hwang ◽

John H. Kehrl

Keyword(s):

Lymph Nodes ◽

B Lymphocyte ◽

Confocal Imaging ◽

Thick Section ◽

Lymphocyte Trafficking ◽

Two Photon

Download Full-text

Adrenergic control of the adaptive immune response by diurnal lymphocyte recirculation through lymph nodes

Journal of Experimental Medicine ◽

10.1084/jem.20160723 ◽

2016 ◽

Vol 213 (12) ◽

pp. 2567-2574 ◽

Cited By ~ 74

Author(s):

Kazuhiro Suzuki ◽

Yuki Hayano ◽

Akiko Nakai ◽

Fumika Furuta ◽

Masaki Noda

Keyword(s):

Immune Response ◽

Immune System ◽

Lymph Nodes ◽

Diurnal Variation ◽

Adaptive Immune Response ◽

Physiological Role ◽

Lymphocyte Trafficking ◽

Adaptive Immune ◽

Adrenergic Control ◽

Lymphocyte Recirculation

Various aspects of the immune system display circadian rhythms. Although lymphocyte trafficking has been suggested to show diurnal variations, the mechanisms and influences on immune responses are unclear. Here, we show in mice that inputs from adrenergic nerves contribute to the diurnal variation of lymphocyte recirculation through lymph nodes (LNs), which is reflected in the magnitude of the adaptive immune response. Neural inputs to β2-adrenergic receptors (β2ARs) expressed on lymphocytes reduced the frequency of lymphocyte egress from LNs at night, which was accompanied by an increase of lymphocyte numbers in LNs. Immunization during the period of lymphocyte accumulation in LNs enhanced antibody responses. The diurnal variation of the humoral immune response was dependent on β2AR-mediated neural signals and was diminished when lymphocyte recirculation through LNs was stopped. This study reveals the physiological role of adrenergic control of lymphocyte trafficking in adaptive immunity and establishes a novel mechanism that generates diurnal rhythmicity in the immune system.

Download Full-text

Rap1 and its effector RIAM are required for lymphocyte trafficking

Blood ◽

10.1182/blood-2015-05-644104 ◽

2015 ◽

Vol 126 (25) ◽

pp. 2695-2703 ◽

Cited By ~ 45

Author(s):

Wenjuan Su ◽

Joseph Wynne ◽

Elaine M. Pinheiro ◽

Marianne Strazza ◽

Adam Mor ◽

...

Keyword(s):

Cell Adhesion ◽

T Cell ◽

Lymph Nodes ◽

T Cell Development ◽

Cell Development ◽

Lymphocyte Function ◽

Lymphocyte Trafficking ◽

Key Points ◽

Late Antigen ◽

T Cell Adhesion

Key Points Rap1 and its effector RIAM are required for integrin-mediated T-cell adhesion and homing to lymph nodes, but not for T-cell development. RIAM regulates the activation of lymphocyte function-associated antigen 1 and very late antigen 4 on lymphocytes, but not αIIbβ3 on platelets.

Download Full-text

Scanning electron microscopy of freeze-fractured prescapular lymph node of caprine

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111331 ◽

1984 ◽

Vol 42 ◽

pp. 244-245

Author(s):

O. Faroon ◽

F. Al-Bagdadi ◽

T. G. Snider ◽

C. Titkemeyer

Keyword(s):

Lymph Node ◽

Lymph Nodes ◽

Lymphatic System ◽

Three Dimensional ◽

Meat Products ◽

The Body ◽

Morphological Data ◽

The World ◽

Cellular Constituent ◽

Scanning Electron

The lymphatic system is very important in the immunological activities of the body. Clinicians confirm the diagnosis of infectious diseases by palpating the involved cutaneous lymph node for changes in size, heat, and consistency. Clinical pathologists diagnose systemic diseases through biopsies of superficial lymph nodes. In many parts of the world the goat is considered as an important source of milk and meat products.The lymphatic system has been studied extensively. These studies lack precise information on the natural morphology of the lymph nodes and their vascular and cellular constituent. This is due to using improper technique for such studies. A few studies used the SEM, conducted by cutting the lymph node with a blade. The morphological data collected by this method are artificial and do not reflect the normal three dimensional surface of the examined area of the lymph node. SEM has been used to study the lymph vessels and lymph nodes of different animals. No information on the cutaneous lymph nodes of the goat has ever been collected using the scanning electron microscope.

Download Full-text

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100141743 ◽

1995 ◽

Vol 53 ◽

pp. 1074-1075

Author(s):

F.E. Hossler ◽

M.I. McKamey ◽

F.C. Monson

Keyword(s):

Lymph Nodes ◽

Light Microscopy ◽

Corrosion Casting ◽

Fixed Tissue ◽

Infusion Pressure ◽

India Ink ◽

Cacodylate Buffer ◽

Rabbit Bladder ◽

Lateral Walls ◽

Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

Download Full-text