Methods for Enrichment and Sequencing of Oral Viral Assemblages: Saliva, Oral Mucosa, and Dental Plaque Viromes

2018 ◽  
pp. 143-161 ◽  
Author(s):  
Marcos Parras-Moltó ◽  
Alberto López-Bueno
Keyword(s):  
Oral Mucosa ◽  
Dental Plaque

scholarly journals Effectiveness and safety of a new dental plaque removal device utilizing micro mist spray for removing oral biofilm in vitro

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hiroki Hihara ◽  
Ryo Tagaino ◽  
Jumpei Washio ◽  
Kittipong Laosuwan ◽  
Dimas Prasetianto Wicaksono ◽  
...  
Keyword(s):  
Surface Roughness ◽  
Mucous Membrane ◽  
Oral Mucosa ◽  
Dental Plaque ◽  
Tooth Surface ◽  
Oral Biofilm ◽  
Plaque Removal ◽  
Treated Area ◽  
Biofilm Removal ◽  
Significant Difference

Abstract Background Removal of oral biofilm from the oral mucosa is essential for preventing risk of respiratory and gastrointestinal infection in elderly people. Currently, no device is available which can remove oral biofilm from oral mucosa effectively and safely. Therefore, the effectiveness and safety of the Micro Scale Mist UNIT (MSM-UNIT), a newly developed dental plaque removal device utilizing high speed sprays of fine water droplets, were evaluated for biofilm removal, including the rate and surface roughness for simulated tooth surface and mucous membrane. Methods Simulated tooth and oral mucosa coated with an artificial biofilm of Streptococcus mutans were used for evaluation of effectiveness, with uncoated substrates as the controls. The MSM-UNIT and a conventional air ablation device were operated under recommended instructions. The effectiveness was evaluated from the rate of removal of the biofilm, and the safety was evaluated from the damage observed by scanning electron microscope and surface roughness. Results The biofilm removal rate of the MSM-UNIT was significantly higher than that of AIRFLOW. Little damage was observed in the area treated by the MSM-UNIT. The surface roughness of the MSM-UNIT treated area on simulated tooth surface and oral mucosa showed no significant difference to the control area. In contrast, cracks and powder were observed in the area treated by AIRFLOW. In particular, the surface roughness of the AIRFLOW treated area for Toughsilon was significantly larger than that of the control. Conclusions The MSM-UNIT could be used safely and effectively for removing biofilm not only on simulated tooth surfaces but also simulated mucous membrane. The MSM-UNIT has no harmful effect on teeth or oral mucosa, and may be used for comprehensive oral care for patients during nursing care and the perioperative period.

Use of an Oral Antiseptic Bioadhesive Tablet in Dogs

1995 ◽  
Vol 12 (3) ◽  
pp. 87-91 ◽  
Author(s):  
P. Gruet ◽  
C. Gaillard ◽  
B. Boisramέ ◽  
D. Duffaut ◽  
A. M. Grimoud ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Dental Plaque ◽  
Bacterial Counts ◽  
Dental Scaling ◽  
Oral Microflora ◽  
Carry Over ◽  
Carry Over Effect

V3703 (Stomadhex®*) is a tablet with bioadhesive properties enabling it to remain in place for several hours after it has been placed on the oral mucosa. It continuously releases chlorhexidine and niacinamide. In a study conducted in 15 dogs, the tablets were well tolerated by the animals. The product significantly reduced (p < 0.05): dental plaque; quantitative periodontopathogen and total anaerobic bacterial counts; spirochetes; and halitosis when used daily over a 14 day period. Gingivitis was also reduced, though not significantly (p=0.07). Stomadhex® treatment can provide a carry-over effect following dental scaling by reducing oral microflora and retarding the reappearance of dental plaque.

The Surface Area of the Adult Human Mouth and Thickness of the Salivary Film Covering the Teeth and Oral Mucosa

1987 ◽  
Vol 66 (8) ◽  
pp. 1300-1302 ◽  
Author(s):  
L.M.C. Collins ◽  
C. Dawes
Keyword(s):  
Surface Area ◽  
Oral Mucosa ◽  
Dental Plaque ◽  
Thick Layer ◽  
Aluminum Foil ◽  
Ventral Surface ◽  
Total Surface Area ◽  
Average Thickness ◽  
Significant Difference ◽  
Keratinized Epithelium

The surface area of the mouth was measured to calculate the average thickness of the salivary film which separates the opposing layers of the oral mucosa and which also overlies the dental plaque. The subjects were 10 adults of each sex, all of whom had essentially a full complement of teeth. Impressions were taken of the upper and lower jaws, the buccal and labial vestibular mucosa, and the ventral surface of the tongue, and stone models were cast. The dorsum of the tongue was assumed to have the same area as the palate plus that of the palatal surfaces of the upper teeth. The six separate areas considered were the teeth, the palate, the buccal and lingual gingival and alveolar mucosa, the buccal and labial vestibular mucosa, the ventral surface of the tongue, including the floor of the mouth, and the dorsum of the tongue. Aluminum foil, of known weight per unit area, was adapted to the models of the different regions of the mouth, and the surface areas were calculated from the weights of the foil. The mean total surface area of the mouth was 214.7 ± 12.9 cm 2, and there was no significant difference due to gender. The teeth, keratinized epithelium, and non-keratinized epithelium occupied about 20%, 50%, and 30% of the total surface area, respectively. Given that the average volumes of saliva present in the mouth before and after swallowing have been estimated to be 0.77 and 1.07 mL, respectively, it can be calculated that the average thickness of the salivary film in the mouth varies between 0.07 and 0.10 mm. Since we have previously assumed that dental plaque is washed by a relatively thick layer of saliva, the results have important implications with regard to diffusion of substances in and out of dental plaque and with regard to the sensation of oral dryness caused by fluid absorption through the oral mucosa or by salivary evaporation.

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Physiology of the Oral Mucosa

1979 ◽  
Vol 12 (1) ◽  
pp. 15-20 ◽  
Author(s):  
William K. Bottomley
Keyword(s):  
Oral Mucosa

Imaging of fibrosis of the oral mucosa by 20 MHz sonography

1999 ◽  
Vol 28 (5) ◽  
pp. 290-294 ◽  
Author(s):  
J Jackowski ◽  
P Jöhren ◽  
A M Müller ◽  
A Kruse ◽  
T Dirschka
Keyword(s):  
Oral Mucosa

Kinetics of protoporphyrin IX formation in rat oral mucosa and skin after application of 5-aminolevulinic acid and its methylester

2004 ◽  
Author(s):  
Stefan Kristiansson ◽  
Asta Juzeniene ◽  
Petras Juzenas ◽  
Vladimir Iani ◽  
Lennart Löfgren ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Aminolevulinic Acid ◽  
Protoporphyrin Ix ◽  
Kinetics Of
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