scholarly journals Effectiveness and safety of a new dental plaque removal device utilizing micro mist spray for removing oral biofilm in vitro

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hiroki Hihara ◽  
Ryo Tagaino ◽  
Jumpei Washio ◽  
Kittipong Laosuwan ◽  
Dimas Prasetianto Wicaksono ◽  
...  

Abstract Background Removal of oral biofilm from the oral mucosa is essential for preventing risk of respiratory and gastrointestinal infection in elderly people. Currently, no device is available which can remove oral biofilm from oral mucosa effectively and safely. Therefore, the effectiveness and safety of the Micro Scale Mist UNIT (MSM-UNIT), a newly developed dental plaque removal device utilizing high speed sprays of fine water droplets, were evaluated for biofilm removal, including the rate and surface roughness for simulated tooth surface and mucous membrane. Methods Simulated tooth and oral mucosa coated with an artificial biofilm of Streptococcus mutans were used for evaluation of effectiveness, with uncoated substrates as the controls. The MSM-UNIT and a conventional air ablation device were operated under recommended instructions. The effectiveness was evaluated from the rate of removal of the biofilm, and the safety was evaluated from the damage observed by scanning electron microscope and surface roughness. Results The biofilm removal rate of the MSM-UNIT was significantly higher than that of AIRFLOW. Little damage was observed in the area treated by the MSM-UNIT. The surface roughness of the MSM-UNIT treated area on simulated tooth surface and oral mucosa showed no significant difference to the control area. In contrast, cracks and powder were observed in the area treated by AIRFLOW. In particular, the surface roughness of the AIRFLOW treated area for Toughsilon was significantly larger than that of the control. Conclusions The MSM-UNIT could be used safely and effectively for removing biofilm not only on simulated tooth surfaces but also simulated mucous membrane. The MSM-UNIT has no harmful effect on teeth or oral mucosa, and may be used for comprehensive oral care for patients during nursing care and the perioperative period.

Author(s):  
Ji-Hyun Lee ◽  
Jin-Hee Ha

This study evaluated the effectiveness of a microcurrent toothbrush (approved by the US Food and Drug Administration [FDA]), which employs a superimposed alternating and direct electric current, named as a Proxywave® technology, similar to the intensity of the biocurrent, in plaque removal and reducing gingivitis by biofilm removal through the bioelectric effect. This study enrolled 40 volunteers with gingivitis. Dental observations were made every two weeks, before and after the use of each toothbrush. We randomly assigned participants into two groups: one group used the Proxywave® toothbrush (PB) for two weeks followed by the control toothbrush (CB) for two weeks, while the other group used the CB for two weeks followed by the PB. The participants had a two-week washout period. If the toothbrush used earlier has had an effect on the bacterial flora in the oral cavity, this is to remove this effect and return it to its previous state. During each dental visit, we recorded plaque index (PI) and gingival index (GI) scores. The PI and GI scores were significantly lower in both the PB and the CB (p < 0.05). Considering the PI, there was no significant difference between the toothbrushes on all the surfaces. Considering the GI, the PB showed a significant decrease in the interproximal surface, compared to the CB (p < 0.05). The PB showed a significant decrease in the interproximal GI and had a beneficial effect in the interproximal area where the bristles could not reach. No adverse events were observed in the participants during the clinical trial. The microcurrent toothbrush is a device that can be safely used for plaque removal.


2021 ◽  
Author(s):  
Burcu Duman ◽  
Salih Doğan

Abstract BackgroundSurface roughness is one of the main factors affecting bacterial adhesion, biofilm growth, plaque formation and coloration on the tooth surface. Improper polishing may increase roughness or not contribute to a reduction in roughness while removing biofilms on tooth surfaces. The aim of this study is to examine the effects of different polishing pastes applied to the enamel of primary teeth on the surface roughness through AFM, which enables a more detailed examination of the measurements, and to evaluate the roughness changes created by the paste on the surface. MethodsIn the present study, in order to compare polishing made using Septodent Détartrine, Kerr Cleanic, Shofu Mersaage Regular under the recommendations of the companies in addition to the application of pumice with a rubber brush, a total of 88 samples with 22 samples in each group were created using 88 primary molar teeth. Before and after polishing, roughness measurements and visualizations were performed with atomic force microscopy (AFM). After polishing, Ra (average roughness) values, which express the surface roughness of the re-scanned samples, were calculated so that the samples were the same as the first scanning. For statistical analysis, Shapiro-Wilk normality test and generalized linear models tests were applied and p <0.05 was considered as statistically significantResultsIn the groups; while there was no significant difference in terms of roughness values ​​(Ra) within the group (p> 0.05), a statistically significant reduction in roughness (Ra) was observed on the sample surfaces after polishing in all groups compared to the baseline (p <0.05). According to the data obtained; all polishing methods used in the treatment of primary tooth discoloration can reduce the plaque formation and thus the amount of recoloring by reducing the surface roughness at a similar level.ConclusionAccordingly, it has been observed that the current polishing pastes do not provide a significant advantage over the traditional polishing method (polishing brush with pumice) in terms of surface roughness in removing discoloration in primary teeth.


Author(s):  
Michele Nieri ◽  
Veronica Giuntini ◽  
Umberto Pagliaro ◽  
Monica Giani ◽  
Lorenzo Franchi ◽  
...  

Background: The aim of this single-use, four-treatment, four-period (visit), cross-over, mono-centered, examiner-blind, cross-over randomized controlled trial (RCT) was to evaluate the efficacy in dental plaque removal of a U-shaped automatic electric toothbrush (U) compared to a conventional powered toothbrush (P), a habitual toothbrushing procedure (H), and no brushing (N). Methods: Eligible participants were volunteer students. Primary outcome measure was the reduction in full-mouth plaque score (FMPS) after brushing. The secondary outcome variable was a visual analogic scale (VAS) on subjective clean mouth sensation. Mixed models were performed for difference in FMPS and VAS. Results: Twenty-two participants were randomized to the treatments in the four periods of the study. The differences between treatments in FMPS reduction after brushing were statistically significant (p < 0.0001). The differences were statistically significant between the U and P groups (difference −48; 95% CI from −54 to −41) favoring the P group, and between the U and H groups (difference −45; 95% CI from −52 to −39) favoring the H group. On the contrary, the difference between the U and N groups was not significant (difference 5; 95% CI from −2 to 12) favoring the U group. The differences between treatments in clean mouth VAS was statistically significant (p < 0.0001) favoring the P and H groups. Conclusions: The U-shaped automatic electric toothbrush tested in this study proved to be not effective in removing dental plaque.


2018 ◽  
Vol 7 (1) ◽  
pp. 28
Author(s):  
Betrik Sefyana Mangiri ◽  
Sinar Yani ◽  
Silvia Anitasari

Dental plaque is a colorless thin layer, is not easily visible on the tooth surface and it causes caries. Disclosing solution is the material that can help us to see dental plaque. Chemical disclosing solution, which now exists, has several disadvantages so we need to develop natural disclosing solution. One of the ingredients in natural disclosing solution that has been studied for its effect is betacyanin, which is contained in super-red dragon fruit (Hylocereus costaricensis). This study aim to observe the coloring effect of super-red dragon fruit juice. The experimental was conducted by measuring plaque index of 20 people, who were not to clean their teeth for 24 hours (two periods), and were eaten the same foods in each period. Examination of plaque index at the end of the first period used chemical disclosing solution contains of erythrosine (FDC Red #3) and the second period used super-red dragon fruit juice. The datas were analyzed by Paired T-test. The results of the plaque index score at the first (2.47815 ± 0.811497), the second (2.7731 ± 1.0512) and did not find a significant difference (p > 0.05). The ability of super-red dragon fruit to coloring dental plaque has no difference with chemical disclosing solution, so it can be used as a natural disclosing solution.


1987 ◽  
Vol 66 (8) ◽  
pp. 1300-1302 ◽  
Author(s):  
L.M.C. Collins ◽  
C. Dawes

The surface area of the mouth was measured to calculate the average thickness of the salivary film which separates the opposing layers of the oral mucosa and which also overlies the dental plaque. The subjects were 10 adults of each sex, all of whom had essentially a full complement of teeth. Impressions were taken of the upper and lower jaws, the buccal and labial vestibular mucosa, and the ventral surface of the tongue, and stone models were cast. The dorsum of the tongue was assumed to have the same area as the palate plus that of the palatal surfaces of the upper teeth. The six separate areas considered were the teeth, the palate, the buccal and lingual gingival and alveolar mucosa, the buccal and labial vestibular mucosa, the ventral surface of the tongue, including the floor of the mouth, and the dorsum of the tongue. Aluminum foil, of known weight per unit area, was adapted to the models of the different regions of the mouth, and the surface areas were calculated from the weights of the foil. The mean total surface area of the mouth was 214.7 ± 12.9 cm 2, and there was no significant difference due to gender. The teeth, keratinized epithelium, and non-keratinized epithelium occupied about 20%, 50%, and 30% of the total surface area, respectively. Given that the average volumes of saliva present in the mouth before and after swallowing have been estimated to be 0.77 and 1.07 mL, respectively, it can be calculated that the average thickness of the salivary film in the mouth varies between 0.07 and 0.10 mm. Since we have previously assumed that dental plaque is washed by a relatively thick layer of saliva, the results have important implications with regard to diffusion of substances in and out of dental plaque and with regard to the sensation of oral dryness caused by fluid absorption through the oral mucosa or by salivary evaporation.


2017 ◽  
Vol 18 (4) ◽  
pp. 300-306 ◽  
Author(s):  
Sabin Salahuddin ◽  
Robel Dam ◽  
Khalid H Zawawi ◽  
Dowen Birkhed

ABSTRACT Introduction Dental caries and periodontal diseases are all induced by oral biofilm (dental plaque). This study was conducted to evaluate if fluoride-impregnated miswak is as effective in plaque removal and fluoride release as toothbrushing with fluoride toothpaste. Materials and methods This single-blind, randomized, crossover study was conducted at the Department of Cariology, University of Gothenburg, Gothenburg, Sweden, from February 2010 to January 2011. Fifteen healthy subjects participated in this study. The participants were instructed to use the following: (1) 0.5% NaF-impregnated miswak, (2) nonfluoridated miswak, (3) toothbrush with nonfluoride toothpaste, and (4) toothbrush with 1450 ppm fluoride toothpaste. Each method was used twice a day for 1 week after which plaque amount and fluoride concentration in resting saliva were measured. There was a 1-week washout period between each method. Results No significant difference between miswak and toothbrushing was found regarding plaque removal on buccal and lingual surfaces. A somewhat higher fluoride concentration in resting saliva was found after using impregnated miswak when compared with toothbrushing with fluoride toothpaste (p < 0.05). Conclusion Miswak and toothbrushing showed the same plaque removing effect on buccal and lingual surfaces. Miswak impregnated with 0.5% NaF resulted in a higher concentration of fluoride in saliva than brushing with 1450 ppm fluoride toothpaste. Clinical significance Miswak impregnated with 0.5% NaF and toothbrushing results in comparable plaque removal and about the same fluoride concentration in saliva even it was somewhat higher for impregnated miswak. How to cite this article Baeshen H, Salahuddin S, Dam R, Zawawi KH, Birkhed D. Comparison of Fluoridated Miswak and Toothbrushing with Fluoridated Toothpaste on Plaque Removal and Fluoride Release. J Contemp Dent Pract 2017;18(4):300-306.


2018 ◽  
Vol 51 (1) ◽  
pp. 33
Author(s):  
Suryani Hutomo ◽  
Heni Susilowati ◽  
Dewi Agustina ◽  
Widya Asmara

Background: Streptococcus sanguinis (S. sanguinis), an oral commensal bacterium, is often implicated in infective endocarditis. Its adherence to the tooth surface is the initial step in dental plaque formation. In addition to the important role of S. sanguinis in systemic disease and antimicrobial resistance, it is necessary to develop methods to control dental plaque formation. Immunoglobulin Y (IgY) has been used to prevent bacterial infection. Purpose: The purpose of this study is to analyze the ability of anti-S. sanguinis IgY antibodies to inhibit S. sanguinis adherence to hydroxyapatite (HA) discs as a model of the tooth surface. Methods: Antibodies were produced by immunizing hens with S. sanguinis suspension. Boosters were given three times following the first injection. An agar gel precipitation test (AGPT) was used to detect the presence of anti-S. sanguinis IgY. A bacterial adherence assay was performed twice to analyze the ability of IgY and the optimal concentration required to inhibit bacterial adherence. Results: The formation of a precipitation line using AGPT confirmed the presence of the antibody. In addition, it was shown that the anti-S. sanguinis IgY antibody could inhibit bacterial adherence to HA. Statistical analysis using One-way ANOVA revealed a significant difference in the optical density (OD) value between the groups (p<0.05). The results of electron microscopy scanning confirmed the quantitative analysis by means of a bacterial adherence test. Conclusion: Anti-S. sanguinis IgY has the ability to inhibit adherence of S. sanguinis to HA discs at an optimal concentration of 30%. The inhibitive effect was stronger in the presence of saliva.


2020 ◽  
Vol 11 (3) ◽  
pp. 559-562
Author(s):  
Mahdieh Zarabadipour ◽  
Reihaneh Saffari ◽  
Monirsadat Mirzadeh

Introduction: Dental plaque removal is an important goal in oral hygiene program. The public interest in herbal medicine and natural products has grown in the past few years. Siwak (Salvadora persica L. (kharijal)) is an ancient tooth stick that has preventive and therapeutic effects on oral cavity. The aim of the present study is to evaluate the effect of siwak chewing sticks on plaque accumulation on tooth surface. Materials and Methods: In this randomized clinical trial study 80 volunteer was selected randomly for complete the study chart. They were enrolled in the study for being in the one of the four study groups: active siwak, inactive siwak, usual tooth brush and no-oral hygiene. The participants wanted to use their instruction for 6 days. Base line (PI-1) and 3-day (PI-2) and 6-day (PI-3) plaque indexes was recorded by Sillness and Loe method. Data evaluation was done using SPSS-22 and Kruskal Wallis Test, Chi-Square Tests. Results: There was a significant reduction of the plaque index in PI-3 (p= 0.029). But in the others, there was no significant difference in the change of plaque index between groups (p>0.05). Conclusion: It is concluded that siwak were able to reduce dental plaque as a mechanical tooth brush and if it uses in active form (fresh plant), the reduction of plaque accumulation is more prominent and effective.


2020 ◽  
Vol 54 (1) ◽  
pp. 44-48
Author(s):  
Pooja Harish S ◽  
Karunakara B C ◽  
Sumitra Reddy

Objective: A vivo study was conducted to evaluate the surface roughness produced by two different methods: hand-held mechanical and air-rotor stripping and also by HORICO and Ortho-Organizer strips (Bengaluru, India), before and after polishing with 3M Sof-Lex Finishing Strips under Atomic Force Microscope. Methodology: Study included 44 proximal surfaces of extracted premolars divided into a control group and 3 experimental groups with 12 surfaces in each. Hand-held mechanical stripping was done by 40 passages of 6 cm long abrasive strips and air-rotor stripping using high-speed air-rotor turbine hand piece. Polishing was done using 3M Sof-Lex finishing strips. Reduced teeth samples were viewed under Atomic Force Microscope and the proximal strips under Confocal microscope for surface roughness. Results: Air-rotor stripping produced statistically significant more surface roughness compared to the mechanical reduction technique ( P = .01). There was no significant difference between the roughnesses produced by 2 different proximal strips. Tooth surface after IPR with polishing had less roughness compared to unpolished surface. There was no mean difference between the wear of proximal strips. Conclusion: The mechanical reduction technique of interproximal surface produces less surface roughness compared to air-rotor stripping. Polishing with 3M Sof-Lex strips after reduction irrespective of the technique and material used gives smoother surface than even normal enamel.


2017 ◽  
Vol 50 (1) ◽  
pp. 1
Author(s):  
Fitria Avriliyanti ◽  
Sri Suparwitri ◽  
Ananto Ali Alhasyimi

Background: Fixed orthodontic appliance patients have a high risk to increasing plaque accumulation in tooth surface due to the presence of fixed appliance components. Using mouthwash with antibacterial agent from herbal material can control dental plaque accumulation. Bay leaf (BL) contains active compounds such as essential oil, tannin and flavonoid that have been known as an antibacterial agent. Purpose: The purpose of this study was to determine the effect of rinsing with 60% of BL aqueous decoction to the accumulation of dental plaque in fixed orthodontic appliance patients. Method: This research was an experimental clinical research with pretest and post-test control group design conducted on 20 subjects with age ranged between 18-25 years old. All subjects were undergoing the last stage (finishing) of fixed active orthodontic treatment. The subjects were instructed to rinse with 60% of BL aqueous decoction and 0.2% chlorhexidine as a control. Wash out period that needed between rinsing with 60% of BL aqueous decoction and rinsing with 0.2% chlorhexidine was 7 days. Each mouthwash used routinely for 7 days with same duration and intensity. Plaque scoring was measured before and after each treatment using Orthodontic Plaque Index (OPI). Result: One-way Anova test showed that there was a significant difference in the plaque index before and after treatment between the group of rinsing with 60% of BL aqueous decoction and group of rinsing with chlorhexidine (p<0.05). Conclusion: Rinsing with 60% BL aqueous decoction can decrease the accumulation of dental plaque in fixed orthodontic appliance patients.


Sign in / Sign up

Export Citation Format

Share Document