Measurement of Phospholipid Metabolism in Intact Neutrophils

2007 ◽  
pp. 69-83 ◽  
Author(s):  
Susan Sergeant ◽  
Linda C. McPhail
Keyword(s):  
Phospholipid Metabolism

scholarly journals Effect of CO2 concentration on phospholipid metabolism in the isolated perfused rat lung

1973 ◽  
Vol 14 (2) ◽  
pp. 145-151
Author(s):  
William J. Longmore ◽  
Carolyn M. Niethe ◽  
Deborah J. Sprinkle ◽  
Rodolfo I. Godinez
Keyword(s):  
Co2 Concentration ◽  
Phospholipid Metabolism ◽  
Rat Lung

scholarly journals RADIOACTIVE PHOSPHORUS AS AN INDICATOR OF PHOSPHOLIPID METABOLISM

1938 ◽  
Vol 126 (2) ◽  
pp. 493-500 ◽  
Author(s):  
G.W. Changus ◽  
I.L. Chaikoff ◽  
S. Ruben
Keyword(s):  
Phospholipid Metabolism ◽  
Radioactive Phosphorus

scholarly journals Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tokuji Tsuji ◽  
Shin-ya Morita ◽  
Yoshinobu Nakamura ◽  
Yoshito Ikeda ◽  
Taiho Kambe ◽  
...  
Keyword(s):  
Cell Growth ◽  
Hepg2 Cells ◽  
Phospholipid Class ◽  
Present Report ◽  
Phospholipid Metabolism ◽  
Cancer Cell Growth ◽  
Microsomal Membranes ◽  
Intracellular Organelles ◽  
The Relationship ◽  
Pe Ratio

AbstractThe human hepatoblastoma cell line, HepG2, has been used for investigating a wide variety of physiological and pathophysiological processes. However, less information is available about the phospholipid metabolism in HepG2 cells. In the present report, to clarify the relationship between cell growth and phospholipid metabolism in HepG2 cells, we examined the phospholipid class compositions of the cells and their intracellular organelles by using enzymatic fluorometric methods. In HepG2 cells, the ratios of all phospholipid classes, but not the ratio of cholesterol, markedly changed with cell growth. Of note, depending on cell growth, the phosphatidic acid (PA) ratio increased and phosphatidylcholine (PC) ratio decreased in the nuclear membranes, the sphingomyelin (SM) ratio increased in the microsomal membranes, and the phosphatidylethanolamine (PE) ratio increased and the phosphatidylserine (PS) ratio decreased in the mitochondrial membranes. Moreover, the mRNA expression levels of enzymes related to PC, PE, PS, PA, SM and cardiolipin syntheses changed during cell growth. We suggest that the phospholipid class compositions of organellar membranes are tightly regulated by cell growth. These findings provide a basis for future investigations of cancer cell growth and lipid metabolism.

scholarly journals Acetyl-CoA carboxylase from yeast is an essential enzyme and is regulated by factors that control phospholipid metabolism

1993 ◽  
Vol 268 (15) ◽  
pp. 10946-10952 ◽  
Author(s):  
M. Hasslacher ◽  
A.S. Ivessa ◽  
F. Paltauf ◽  
S.D. Kohlwein
Keyword(s):  
Phospholipid Metabolism ◽  
Acetyl Coa Carboxylase ◽  
Acetyl Coa ◽  
Essential Enzyme

scholarly journals Effects of Natural Antioxidants on Phospholipid and Ceramide Profiles of 3D-Cultured Skin Fibroblasts Exposed to UVA or UVB Radiation

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 578
Author(s):  
Agnieszka Gęgotek ◽  
Wojciech Łuczaj ◽  
Elżbieta Skrzydlewska
Keyword(s):  
Ascorbic Acid ◽  
High Performance ◽  
Three Dimensional ◽  
Natural Antioxidants ◽  
Phospholipid Metabolism ◽  
Culture Conditions ◽  
Membrane Properties ◽  
Dimensional System ◽  
Uvb Radiation ◽  
Liquid Chromatograph

Ultraviolet (UV) radiation is one of the primary factors responsible for disturbances in human skin cells phospholipid metabolism. Natural compounds that are commonly used to protect skin, due to their lipophilic or hydrophilic nature, show only a narrow range of cytoprotective activity, which prompts research on their combined application. Therefore, the aim of this study was to examine the effect of ascorbic acid and rutin on the phospholipid and ceramide profiles in UV-irradiated fibroblasts cultured in a three-dimensional system that approximates the culture conditions to the dermis. An ultra-high-performance liquid chromatograph coupled with a quadrupole time-of-flight mass spectrometer was used for phospholipid and ceramide profiling. As a result of UVA and UVB cells irradiation, upregulation of phosphatidylcholines, ceramides, and downregulation of sphingomyelins were observed, while treatment with ascorbic acid and rutin of UVA/UVB-irradiated fibroblast promoted these changes to provide cells a stronger response to stress. Moreover, an upregulation of phosphatidylserines in cells exposed to UVB and treated with both antioxidants suggests the stimulation of UV-damaged cells apoptosis. Our findings provide new insight into action of rutin and ascorbic acid on regulation of phospholipid metabolism, which improves dermis fibroblast membrane properties.

scholarly journals Phospholipid metabolism during bacterial growth

1969 ◽  
Vol 10 (2) ◽  
pp. 220-233
Author(s):  
David C. White ◽  
Anne N. Tucker
Keyword(s):  
Bacterial Growth ◽  
Phospholipid Metabolism
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