Systematic Characterization of the Protein Interaction Network and Protein Complexes in Saccharomyces cerevisiae Using Tandem Affinity Purification and Mass Spectrometry

2009 ◽  
pp. 187-207 ◽  
Author(s):  
Mohan Babu ◽  
Nevan J. Krogan ◽  
Donald E. Awrey ◽  
Andrew Emili ◽  
Jack F. Greenblatt
Keyword(s):  
Mass Spectrometry ◽  
Saccharomyces Cerevisiae ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Interaction Network ◽  
Tandem Affinity Purification

scholarly journals The Aryl-Hydrocarbon Receptor Protein Interaction Network (AHR-PIN) as Identified by Tandem Affinity Purification (TAP) and Mass Spectrometry

2013 ◽  
Vol 2013 ◽  
pp. 1-12 ◽  
Author(s):  
Dorothy M. Tappenden ◽  
Hye Jin Hwang ◽  
Longlong Yang ◽  
Russell S. Thomas ◽  
John J. LaPres
Keyword(s):  
Mass Spectrometry ◽  
Gene Regulation ◽  
Aryl Hydrocarbon Receptor ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Affinity Purification ◽  
Interaction Network ◽  
Tandem Affinity Purification ◽  
Toxic Response ◽  
Aryl Hydrocarbon

The aryl-hydrocarbon receptor (AHR), a ligand activated PAS superfamily transcription factor, mediates most, if not all, of the toxicity induced upon exposure to various dioxins, dibenzofurans, and planar polyhalogenated biphenyls. While AHR-mediated gene regulation plays a central role in the toxic response to dioxin exposure, a comprehensive understanding of AHR biology remains elusive. AHR-mediated signaling starts in the cytoplasm, where the receptor can be found in a complex with the heat shock protein of 90 kDa (Hsp90) and the immunophilin-like protein, aryl-hydrocarbon receptor-interacting protein (AIP). The role these chaperones and other putative interactors of the AHR play in the toxic response is not known. To more comprehensively define the AHR-protein interaction network (AHR-PIN) and identify other potential pathways involved in the toxic response, a proteomic approach was undertaken. Using tandem affinity purification (TAP) and mass spectrometry we have identified several novel protein interactions with the AHR. These interactions physically link the AHR to proteins involved in the immune and cellular stress responses, gene regulation not mediated directly via the traditional AHR:ARNT heterodimer, and mitochondrial function. This new insight into the AHR signaling network identifies possible secondary signaling pathways involved in xenobiotic-induced toxicity.

scholarly journals Deciphering Protein Complexes and Protein Interaction Networks by Tandem Affinity Purification and Mass Spectrometry

2002 ◽  
Vol 1 (3) ◽  
pp. 204-212 ◽  
Author(s):  
Anna Shevchenko ◽  
Daniel Schaft ◽  
Assen Roguev ◽  
W. W. M. Pim Pijnappel ◽  
A. Francis Stewart ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Protein Interaction ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Tandem Affinity Purification ◽  
Protein Interaction Networks ◽  
Interaction Networks

scholarly journals Low throughput protocol for immunoprecipitation followed by mass spectrometry of cells stably expressing an HA-tagged protein v1

2020 ◽  
Author(s):  
Harper not provided not provided JW
Keyword(s):  
Mass Spectrometry ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Gene Editing ◽  
Protein Complexes ◽  
Interaction Network ◽  
Epitope Tag ◽  
Powerful Approach

Analysis of protein complexes by mass spectrometry provides a powerful approach for identifying proteins that associate with other proteins. Frequently, this can be done by expressing the protein of interest with an epitope tag, such as a Hemagglutinin-A (HA) epitope, using either a stably expressed lentivirus or by gene editing the HA epitope into the gene of interest. The protocol has been used extensively to create the Bioplex protein interaction network [Huttlin et al Nature. 545:505-509 (2017); Huttlin et al Cell, 162: 425-440 (2015)].

scholarly journals Tandem Affinity Purification and Mass Spectrometry (TAP‐MS) for the Analysis of Protein Complexes

2019 ◽  
Vol 96 (1) ◽  
Author(s):  
Guillaume Adelmant ◽  
Brijesh K. Garg ◽  
Maria Tavares ◽  
Joseph D. Card ◽  
Jarrod A. Marto
Keyword(s):  
Mass Spectrometry ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Tandem Affinity Purification

scholarly journals Probing the Protein Interaction Network of Pseudomonas aeruginosa Cells by Chemical Cross-Linking Mass Spectrometry

Structure ◽  
2015 ◽  
Vol 23 (4) ◽  
pp. 762-773 ◽  
Author(s):  
Arti T. Navare ◽  
Juan D. Chavez ◽  
Chunxiang Zheng ◽  
Chad R. Weisbrod ◽  
Jimmy K. Eng ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Pseudomonas Aeruginosa ◽  
Protein Interaction ◽  
Protein Interaction Network ◽  
Interaction Network ◽  
Cross Linking ◽  
Chemical Cross Linking
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