Simultaneous Determination and Quantification of 12 Benzodiazepines in Serum or Whole Blood Using UPLC/MS/MS

2009 ◽  
pp. 107-119 ◽  
Author(s):  
Josh Gunn ◽  
Scott Kriger ◽  
Andrea R. Terrell
Keyword(s):  
Simultaneous Determination ◽  
Whole Blood

scholarly journals Simultaneous Determination of Cyclosporine A, Tacrolimus, Sirolimus, and Everolimus in Whole-Blood Samples by LC-MS/MS

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Mustafa Karapirli ◽  
Murat Kizilgun ◽  
Ozgur Yesilyurt ◽  
Husamettin Gul ◽  
Zeki Ilker Kunak ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Simultaneous Determination ◽  
Cyclosporine A ◽  
Whole Blood ◽  
Immunosuppressive Drugs ◽  
Tandem Mass ◽  
Blood Samples ◽  
Whole Blood Samples

Objectives. Cyclosporine A (CyA), tacrolimus (TRL), sirolimus (SIR), and everolimus (RAD) are immunosuppressive drugs frequently used in organ transplantation. Our aim was to confirm a robust sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determination of CyA, TRL, SIR, and RAD in whole-blood samples.Materials and Methods. We used an integrated online solid-phase extraction-LC-MS/MS system and atmospheric pressure ionization tandem mass spectrometry (API-MS/MS) in the multiple reaction monitoring (MRM) detection mode. CyA, TRL, SIR, and RAD were simultaneously analyzed in whole blood treated with precipitation reagent taken from transplant patients.Results. System performance parameters were suitable for using this method as a high-throughput technique in clinical practice. The high concentration of one analyte in the sample did not affect the concentration of other analytes. Total analytical time was 2.5 min, and retention times of all analytes were shorter than 2 minutes.Conclusion. This LC-MS/MS method can be preferable for therapeutic drug monitoring of these immunosuppressive drugs (CyA, TRL, SRL, and RAD) in whole blood. Sample preparation was too short and simple in this method, and it permits robust, rapid, sensitive, selective, and simultaneous determination of these drugs.

Simultaneous determination of methotrexate and its eight metabolites in human whole blood by capillary zone electrophoresis

2003 ◽  
Vol 1014 (1-2) ◽  
pp. 93-101 ◽  
Author(s):  
Chien-Yuan Kuo ◽  
Hsin-Lung Wu ◽  
Hwang-Shang Kou ◽  
Shyh-Shin Chiou ◽  
Deng-Chyang Wu ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Capillary Zone Electrophoresis ◽  
Whole Blood ◽  
Human Whole Blood ◽  
Zone Electrophoresis ◽  
Capillary Zone

Simultaneous Determination and Validation of 5F-ADBICA and 5F-NPB-22 in Whole Blood and Urine by LC/MS–MS

2020 ◽  
Vol 83 (10) ◽  
pp. 1283-1291
Author(s):  
Pınar Efeoglu Ozseker ◽  
Nebile Daglıoglu
Keyword(s):  
Simultaneous Determination ◽  
Whole Blood
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