Engineering High Yields of Secondary Metabolites in Rubia Cell Cultures Through Transformation with Rol Genes

2010 ◽  
pp. 229-242 ◽  
Author(s):  
Victor P. Bulgakov ◽  
Yuri N. Shkryl ◽  
Galina N. Veremeichik
Keyword(s):  
Secondary Metabolites ◽  
Cell Cultures ◽  
Rol Genes ◽  
High Yields

Obtaining and Characterization of Alhagi persarum Boiss. et Buhse Callus Cell Cultures, Isoflavonoid Producers

2020 ◽  
Vol 36 (6) ◽  
pp. 35-48
Author(s):  
D.V. Коchkin ◽  
G.I. Sobolkovа ◽  
А.А. Fоmеnkov ◽  
R.А. Sidorov ◽  
А.М. Nоsоv
Keyword(s):  
Fatty Acids ◽  
Cell Culture ◽  
Liquid Chromatography ◽  
Secondary Metabolites ◽  
Cell Cultures ◽  
Mass Spectrometric ◽  
Mass Spectrometric Detection ◽  
Gas Liquid Chromatography ◽  
Callus Cell

The physiological characteristics of the callus cell cultures of Alhagi persarum Boiss et Buhse, a member of the legume family, widely used in folk medicine, have been studied. It was shown that the source of the explant was an important factor in the initiation of callusogenesis: more intense callusogenesis (almost 100%) was observed for explants from various organs of sterile seedlings, rather than intact plants (less than 30%). As a result, more than 20 lines of morphologically different callus cell cultures were obtained, and the growth parameters for the 5 most intensively growing lines were determined. The composition of fatty acids (FA) of total lipids and secondary metabolites in the most physiologically stable callus line Aр-207 was analyzed. Using capillary gas-liquid chromatography with mass spectrometric detection (GLC-MS), 19 individual C12--C24 FAs were identified, the main fraction of which were palmitic (~ 23%), stearic (~ 22%), linoleic (~ 14%) and α-linolenic (~ 33%) acids. The established atypical ratio of FAs (a simultaneous high content of both saturated FAs and polyunsaturated α-linolenic acid) is possibly due to the adaptation of cells to in vitro growth conditions. Phytochemical analysis of the secondary metabolites was carried out using ultra-performance liquid chromatography with electrospray ionization mass spectrometric detection (UPLC MS). Compounds belonging to different structural groups of isoflavones were found. Aglycones (calycosin, formononetin and afrormosin isomer), glucosides (formononetin glucoside), as well as esters of glucosides (malonylglycosides of calicosin, formononetin, afrormosin isomers, glycitein and genistein) were detected. These secondary metabolites are widespread in plants of the Fabaceae family; however, isoflavones are rare in representatives of the Alhagi genus. The presence of malonylated isoflavone glycosides in Alhagi spp. was shown for the first time. endemic plant species, Alhagi, in vitro cell culture, callus cell culture, isoflavones, fatty acids All studies were carried out using the equipment of the "Experimental Biotechnological Facility" and the "All-Russian Collection of Cell Cultures of Higher Plants" of IРР RAS. This work was supported by the Russian Foundation for Basic Research (RFBR), contract no.18-54-06021 (Az_a), and the Government of the Russian Federation, Megagrant Project no. 075-15-2019-1882.

Production of Secondary Metabolites in Cell Cultures of Some Terpenoid-Indole Alkaloids Producing Plants

1987 ◽  
pp. 485-494
Author(s):  
R. Verpoorte ◽  
R. Wijnsma ◽  
P. A. A. Harkes ◽  
H. J. G. ten Hoopen ◽  
J. J. Meijer ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cell Cultures ◽  
Indole Alkaloids ◽  
Terpenoid Indole Alkaloids

Secondary Metabolites in Cell Cultures of Tabernaemontana Species

1989 ◽  
pp. 138-148
Author(s):  
R. Verpoorte ◽  
R. van der Heijden ◽  
J. Schripsema ◽  
M. Sierra ◽  
E. J. M. Pennings ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Cell Cultures

scholarly journals The Health Promoting Bioactivities of Lactuca sativa can be Enhanced by Genetic Modulation of Plant Secondary Metabolites

Metabolites ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 97 ◽  
Author(s):  
Hammad Ismail ◽  
Anna L. Gillespie ◽  
Danielle Calderwood ◽  
Haroon Iqbal ◽  
Colene Gallagher ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Lactuca Sativa ◽  
Genetic Manipulation ◽  
Sesquiterpene Lactones ◽  
Plant Secondary Metabolites ◽  
Rol Genes ◽  
In Vitro Bioactivity ◽  
New Paradigm ◽  
Plant Metabolites

Plant secondary metabolites are protective dietary constituents and rol genes evidently increase the synthesis of these versatile phytochemicals. This study subjected a globally important vegetable, lettuce (Lactuca sativa) to a combination of untargeted metabolomics (LC-QTof-MS) and in vitro bioactivity assays. Specifically, we examined the differences between untransformed cultured lettuce (UnT), lettuce transformed with either rolABC (RA) or rolC (RC) and commercially grown (COM) lettuce. Of the 5333 metabolite features aligned, deconvoluted and quantified 3637, 1792 and 3737 significantly differed in RA, RC and COM, respectively, compared with UnT. In all cases the number of downregulated metabolites exceeded the number increased. In vitro bioactivity assays showed that RA and RC (but not COM) significantly improved the ability of L. sativa to inhibit α-glucosidase, inhibit dipeptidyl peptidase-4 (DPP-4) and stimulate GLP-1 secretion. We putatively identified 76 lettuce metabolites (sesquiterpene lactones, non-phenolic and phenolic compounds) some of which were altered by several thousand percent in RA and RC. Ferulic acid levels increased 3033–9777%, aminooxononanoic acid increased 1141–1803% and 2,3,5,4′tetrahydroxystilbene-2-O-β-d-glucoside increased 40,272–48,008%. Compound activities were confirmed using commercially obtained standards. In conclusion, rol gene transformation significantly alters the metabolome of L.sativa and enhances its antidiabetic properties. There is considerable potential to exploit rol genes to modulate secondary metabolite production for the development of novel functional foods. This investigation serves as a new paradigm whereby genetic manipulation, metabolomic analysis and bioactivity techniques can be combined to enable the discovery of novel natural bioactives and determine the functional significance of plant metabolites.

PRODUCTION OF SECONDARY METABOLITES IN PLANT CELL CULTURES

Planta Medica ◽  
1974 ◽  
Vol 25 (02) ◽  
pp. 158-165 ◽  
Author(s):  
F. Constabel ◽  
O. Gamborg ◽  
W. Kurz ◽  
W. Steck
Keyword(s):  
Secondary Metabolites ◽  
Plant Cell ◽  
Cell Cultures ◽  
Plant Cell Cultures
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