In Situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated Antigens

As one of the major types of biomacromolecules in the cell, glycans play essential functional roles in various biological processes. Compared with proteins and nucleic acids, the analysis of glycans in situ has been more challenging. Herein we review recent advances in the development of methods and strategies for labeling, imaging, and profiling of glycans in cells and in vivo. Cellular glycans can be labeled by affinity-based probes, including lectin and antibody conjugates, direct chemical modification, metabolic glycan labeling, and chemoenzymatic labeling. These methods have been applied to label glycans with fluorophores, which enables the visualization and tracking of glycans in cells, tissues, and living organisms. Alternatively, labeling glycans with affinity tags has enabled the enrichment of glycoproteins for glycoproteomic profiling. Built on the glycan labeling methods, strategies enabling cell-selective and tissue-specific glycan labeling and protein-specific glycan imaging have been developed. With these methods and strategies, researchers are now better poised than ever to dissect the biological function of glycans in physiological or pathological contexts.

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Chemical Modification of Metal Oxide Surfaces in Supercritical CO2: In Situ Infrared Studies of the Adsorption and Reaction of Organosilanes on Silica

Langmuir ◽

10.1021/la990495+ ◽

1999 ◽

Vol 15 (22) ◽

pp. 7870-7875 ◽

Cited By ~ 51

Author(s):

J. R. Combes ◽

L. D. White ◽

C. P. Tripp

Keyword(s):

Metal Oxide ◽

Chemical Modification ◽

Supercritical Co2 ◽

Oxide Surfaces ◽

Metal Oxide Surfaces ◽

Infrared Studies

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Feature-Level MALDI-MS Characterization of in Situ-Synthesized Peptide Microarrays

Langmuir ◽

10.1021/la903510y ◽

2010 ◽

Vol 26 (3) ◽

pp. 1456-1459 ◽

Cited By ~ 7

Author(s):

Matthew P. Greving ◽

Pallav Kumar ◽

Zhan-Gong Zhao ◽

Neal W. Woodbury

Keyword(s):

Maldi Ms ◽

Peptide Microarrays

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Effect of in situ expression of human interleukin-6 on antibody responses againstSalmonella typhimuriumantigens

FEMS Immunology & Medical Microbiology ◽

10.1016/s0928-8244(03)00066-x ◽

2003 ◽

Vol 37 (2-3) ◽

pp. 135-145 ◽

Cited By ~ 8

Author(s):

Yuanyi Li ◽

Kerstin Reichenstein ◽

Ramona Ullrich ◽

Tobias Danner ◽

Bernd-Ulrich Specht ◽

...

Keyword(s):

Interleukin 6 ◽

Antibody Responses

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Tyrosine-Specific Chemical Modification with in Situ Hemin-Activated Luminol Derivatives

ACS Chemical Biology ◽

10.1021/acschembio.5b00440 ◽

2015 ◽

Vol 10 (11) ◽

pp. 2633-2640 ◽

Cited By ~ 37

Author(s):

Shinichi Sato ◽

Kosuke Nakamura ◽

Hiroyuki Nakamura

Keyword(s):

Chemical Modification ◽

Specific Chemical ◽

Specific Chemical Modification

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Peptide microarray based analysis of antibody responses to SARS-CoV-2 identifies unique epitopes with potential for diagnostic test development

10.1101/2020.11.24.20216663 ◽

2020 ◽

Author(s):

Pavlo Holenya ◽

Paul Joris Lange ◽

Ulf Reimer ◽

Wolfram Woltersdorf ◽

Thomas Panterodt ◽

...

Keyword(s):

Diagnostic Test ◽

Humoral Immunity ◽

Cross Reactivity ◽

Antibody Responses ◽

Immune Protection ◽

Peptide Microarrays ◽

Igg Binding ◽

Human Coronaviruses ◽

Antibody Tests ◽

Control Samples

SummaryHumoral immunity to the Severe Adult Respiratory Syndrome (SARS) Coronavirus (CoV)-2 is not fully understood yet but may be a crucial factor of immune protection. The possibility of antibody cross-reactivity between SARS-CoV-2 and other human coronaviruses (HCoVs) would have important implications for immune protection but also for the development of specific diagnostic ELISA tests. Using peptide microarrays, n=24 patient samples and n=12 control samples were screened for antibodies against the entire SARS-CoV-2 proteome as well as the Spike (S), Nucleocapsid (N), VME1 (V), R1ab, and Protein 3a (AP3A) of the HCoV strains SARS, MERS, OC43 and 229E. While widespread cross-reactivity was revealed across several immunodominant regions of S and N, IgG binding to several SARS-CoV-2-derived peptides provided statistically significant discrimination between COVID-19 patients and controls. Selected target peptides may serve as capture antigens for future, highly COVID-19-specific diagnostic antibody tests.

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Combined Chemical Modification of Bamboo Material Prepared Using Vinyl Acetate and Methyl Methacrylate: Dimensional Stability, Chemical Structure, and Dynamic Mechanical Properties

Polymers ◽

10.3390/polym11101651 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1651 ◽

Cited By ~ 4

Author(s):

Saisai Huang ◽

Qiufang Jiang ◽

Bin Yu ◽

Yujing Nie ◽

Zhongqing Ma ◽

...

Keyword(s):

Mechanical Properties ◽

Thermal Stability ◽

Chemical Modification ◽

Methyl Methacrylate ◽

Vinyl Acetate ◽

Dimensional Stability ◽

Chemical Structure ◽

In Situ Polymerization ◽

Dynamic Mechanical Properties

Acetylation and in situ polymerization are two typical chemical modifications that are used to improve the dimensional stability of bamboo. In this work, the combination of chemical modification of vinyl acetate (VA) acetylation and methyl methacrylate (MMA) in situ polymerization of bamboo was employed. Performances of the treated bamboo were evaluated in terms of dimensional stability, wettability, thermal stability, chemical structure, and dynamic mechanical properties. Results show that the performances (dimensional stability, thermal stability, and wettability) of bamboo that was prepared via the combined pretreatment of VA and MMA (VA/MMA-B) were better than those of raw bamboo, VA single-treated bamboo (VA-B), and MMA single-treated bamboo (MMA-B). According to scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) analyses, VA and MMA were mainly grafted onto the surface of the cell wall or in the bamboo cell lumen. The antiswelling efficiency and contact angle of VA/MMA-B increased to maximum values of 40.71% and 107.1°, respectively. From thermogravimetric analysis (TG/DTG curves), the highest onset decomposition temperature (277 °C) was observed in VA/MMA-B. From DMA analysis, the storage modulus (E’) of VA/MMA-B increased sharply from 15,057 Pa (untreated bamboo) to 17,909 Pa (single-treated bamboo), and the glass transition temperature was improved from 180 °C (raw bamboo) to 205 °C (single-treated bamboo).

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