Design, Construction, and Analysis of Cell Line Arrays and Tissue Microarrays for Gene Expression Analysis

2011 ◽  
pp. 139-153 ◽  
Author(s):  
Kathy Gately ◽  
Keith Kerr ◽  
Ken O’Byrne
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Tissue Microarrays ◽  
Design Construction

scholarly journals From Gene Expression Analysis to Tissue Microarrays

2006 ◽  
Vol 5 (6) ◽  
pp. 1072-1081 ◽  
Author(s):  
Sara Ek ◽  
Ulrika Andréasson ◽  
Sophia Hober ◽  
Caroline Kampf ◽  
Fredrik Pontén ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Tissue Microarrays

Genome-wide gene expression analysis of human myelomonocytic cell line THP-1 exposed to lipopolysaccharide (LPS)

2008 ◽  
pp. 293-294
Author(s):  
Masayuki Taira ◽  
Minoru Sasaki ◽  
Shigenobu Kimura ◽  
Yoshima Araki
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Genome Wide ◽  
Genome Wide Gene Expression

Gene Expression Analysis of a Human Lymphoblastoma Cell Line ExposedIn Vitroto an Intermittent 1.9 GHz Pulse-Modulated Radiofrequency Field

2006 ◽  
Vol 165 (4) ◽  
pp. 424-429 ◽  
Author(s):  
V. Chauhan ◽  
A. Mariampillai ◽  
P. V. Bellier ◽  
S. S. Qutob ◽  
G. B. Gajda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Radiofrequency Field

Identification of regulatory pathways across different cell lines (NHBE and PBMC) in COVID-19 patients using transcriptome analysis v1

2021 ◽  
Author(s):  
Lavanya not provided C ◽  
Vidya Niranjan ◽  
Aajnaa not provided Upadhyaya ◽  
Arpita not provided Guha Neogi
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Cell Lines ◽  
Expression Analysis ◽  
Pathway Analysis ◽  
Gene Expression Analysis ◽  
Reference Genome ◽  
Host Cells ◽  
Human Reference Genome ◽  
Regulatory Pathways

The Sars-CoV-2 virus is a previously uncharacterized coronavirus and causative agent of the COVID-19 pandemic. Gene expression analysis followed by pathway analysis helps researchers to find possible key targets present in biological pathways of host cells that are targeted by the SARS-CoV-2 virus. This review considers the peripheral blood mononuclear cell line (PBMC) and the normal human bronchial epithelial (NHBE) cell line, both of which support SARS-CoV-2 viral replication. Pathway analysis between the healthy and patient samples of the respective cell lines shall provide useful insights on the COVID-19 disease. Initially, the datasets from the respective cell lines were collected from the NCBI databank. These datasets underwent further analysis and were mapped and aligned to the human reference genome. This outputs the file in the BAM format. The BAM files along with the human reference genome in the GFF format were uploaded to an open-source software called OmicsBox 2.0 for differential gene expression analysis. This resulted in the generation of a table containing the differentially expressed genes which were upregulated and downregulated. These gene lists were uploaded to various pathway analyzers that map the significant genes to the most significant pathways. In this project, KOBAS 3.0 and Enrichr were used for pathway analysis. The pathways obtained from the above-mentioned pathway analyzers were further narrowed down by manual comparison. It was observed that many pathways were similar between the NHBE and PBMC cell lines. However, they were also different in terms of their overall nature. In this project, many patterns were seen through the pathways obtained, however, further optimization and functionality studies must be performed in order to establish conclusive results on the scope of the COVID-19 disease. Expanding research on the scope of the disease by going back to the basics will generate new and valuable information about the virus. This knowledge will help us combat the disease in a better and appropriate manner.

Gene expression analysis of a human enterocyte cell line reveals downregulation of cholesterol biosynthesis in response to short-chain fatty acids

IUBMB Life ◽  
2008 ◽  
Vol 60 (11) ◽  
pp. 757-764 ◽  
Author(s):  
Adriana Alvaro ◽  
Rosa Solà ◽  
Roser Rosales ◽  
Josep Ribalta ◽  
Anna Anguera ◽  
...  
Keyword(s):  
Gene Expression ◽  
Fatty Acids ◽  
Cell Line ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Cholesterol Biosynthesis ◽  
Short Chain Fatty Acids ◽  
Short Chain ◽  
Human Enterocyte ◽  
Chain Fatty Acids

scholarly journals Differential Gene Expression Analysis in K562 Human Leukemia Cell Line Treated with Benzene

2011 ◽  
Vol 27 (1) ◽  
pp. 43-48
Author(s):  
Sul-Ji Choi ◽  
Ji-Young Kim ◽  
Jai-Dong Moon ◽  
Hee-Jo Baek ◽  
Hoon Kook ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Human Leukemia ◽  
Differential Gene Expression Analysis ◽  
Human Leukemia Cell Line ◽  
Differential Gene

Genome-Wide Gene Expression Analysis of NIH 3T3 Cell Line Under Mechanical Stimulation

2010 ◽  
Vol 4 (1) ◽  
pp. 46-55 ◽  
Author(s):  
E. Kurulgan Demirci ◽  
T. Demirci ◽  
J. Trzewik ◽  
P. Linder ◽  
G. Karakulah ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Expression Analysis ◽  
Mechanical Stimulation ◽  
Gene Expression Analysis ◽  
Genome Wide ◽  
Nih 3T3 ◽  
Genome Wide Gene Expression
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