Enrichment of Oral Mucosa and Skin Keratinocyte Progenitor/Stem Cells

2013 ◽  
pp. 293-303 ◽  
Author(s):  
Kenji Izumi ◽  
Cynthia L. Marcelo ◽  
Stephen E. Feinberg
Keyword(s):  
Stem Cells ◽  
Oral Mucosa

scholarly journals Examination of the Therapeutic Potential of Mouse Oral Mucosa Stem Cells in a Wound-Healing Diabetic Mice Model

2020 ◽  
Vol 17 (13) ◽  
pp. 4854
Author(s):  
Shiri Kuperman ◽  
Ram Efraty ◽  
Ina Arie ◽  
Arkadi Rahmanov ◽  
Marina Rahmanov Gavrielov ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Oral Mucosa ◽  
Therapeutic Potential ◽  
Mice Model ◽  
Healing Response ◽  
Wound Healing Response ◽  
Diabetic Wound Healing ◽  
Diabetic Wounds ◽  
Enhance Wound Healing

Diabetic wounds’ delayed healing response is still considered a major therapeutic challenge. Stem cells and derived cellular products have been an active field of research for novel therapies referred to as regenerative medicine. It has recently been shown that human oral mucosa stem cells (hOMSCs) are a readily accessible source for obtaining large quantities of stem cells. This study evaluates the potential of mouse oral mucosa stem cells (mOMSCs) to enhance wound healing in a diabetic (db/db) mouse model by morphological and histological analysis. We show that mOMSCs-treated wounds displayed a significantly faster wound-healing response (p ≤ 0.0001), featuring faster re-epithelialization and a larger area of granulation tissue (p ≤ 0.05). Taken together, these results suggest that oral mucosa stem cells might have therapeutic potential in diabetic wound healing.

scholarly journals Astrocyte-Like Cells Derived From Human Oral Mucosa Stem Cells Provide Neuroprotection In Vitro and In Vivo

2014 ◽  
Vol 3 (3) ◽  
pp. 375-386 ◽  
Author(s):  
Javier Ganz ◽  
Ina Arie ◽  
Tali Ben-Zur ◽  
Michal Dadon-Nachum ◽  
Sammy Pour ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oral Mucosa

scholarly journals Oral mucosa stem cells alleviates spinal cord injury-induced neurogenic bladder symptoms in rats

2014 ◽  
Vol 21 (1) ◽  
pp. 43 ◽  
Author(s):  
Young-Sam Cho ◽  
Il-Gyu Ko ◽  
Sung-Eun Kim ◽  
Sung-Min Lee ◽  
Mal-Soon Shin ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Neurogenic Bladder ◽  
Oral Mucosa ◽  
Cord Injury ◽  
Bladder Symptoms

Generation of human induced pluripotent stem cells from oral mucosa

2010 ◽  
Vol 110 (3) ◽  
pp. 345-350 ◽  
Author(s):  
Keiko Miyoshi ◽  
Daisuke Tsuji ◽  
Keiko Kudoh ◽  
Kazuhito Satomura ◽  
Taro Muto ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Oral Mucosa ◽  
Pluripotent Stem Cells ◽  
Induced Pluripotent

scholarly journals A Preliminary Study of Constructing Tissue Engineered Oral Mucosa

2021 ◽  
Author(s):  
Qin Zhang ◽  
Yu-Jing Tian ◽  
Tao Ding ◽  
De-Hong Femg
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Small Intestine ◽  
Oral Mucosa ◽  
Porous Membrane ◽  
Adipose Derived Stem Cells ◽  
Oral Keratinocytes ◽  
Cell Scaffold ◽  
Compound Graft

Abstract Background: Adipose derived stem cells (ADSCs) have a great potential for tissue-engineering purposes, and they may be introduced in oral mucosa tissue engineering for urethroplasty. This study was aim to develop a tissue-engineered oral mucosa through seeding oral keratinocytes (OKs) and adipose derived stem cells (ADSCs) on small intestine submucosa (SIS). Methods: SIS was obtained from porcine small intestine, and OKs and ADSCs were obtained from canine sources and were cultured and expanded in vitro. The two cell lines were seeded on the two surfaces of the SIS, and the cell-scaffold compound graft was cultured in an air fluid level for 1 week.Results: The SIS exhibited a porous membrane, and no cells were found through HE staining. The model cultured with OK-SIS only formed a thin and loose epithelium. Whereas the model cultured with OK-SIS-ADSC was much thicker and denser. Conclusion: The co-cultured of ADSC and OK grew well on the SIS in which the OKs formed a multilayer of epithelium. So it is feasible to construct a tissue-engineered oral mucosa graft with ADSCs, OKs and SIS. The ADSCs contribute to a thicker epithelium formation.

scholarly journals A Preliminary Study of Constructing Tissue Engineered Oral Mucosa

2021 ◽  
Vol 7 (6) ◽  
Author(s):  
Zhang Q ◽  
Tian YJ ◽  
Ding T ◽  
Feng DH
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Small Intestine ◽  
Oral Mucosa ◽  
Porous Membrane ◽  
Adipose Derived Stem Cells ◽  
Oral Keratinocytes ◽  
Cell Scaffold ◽  
Compound Graft

Adipose derived stem cells (ADSCs) have a great potential for tissue-engineering purposes, and they may be introduced in oral mucosa tissue engineering for urethroplasty. This study was aimed to develop a tissue-engineered oral mucosa through seeding oral keratinocytes (OKs) and adipose derived stem cells (ADSCs) on small intestine submucosa (SIS). From August 2018 to October 2019, an observational study was conducted in the laboratory of our hospital, to develop a tissue-engineered oral mucosa.SIS was obtained from porcine small intestine, and OKs and ADSCs were obtained from canine sources and were cultured and expanded in vitro. The two cell lines were seeded on the two surfaces of the SIS, and the cell-scaffold compound graft was cultured in an air fluid level for 1 week. The SIS exhibited a porous membrane, and no cells were found through HE staining. The model cultured with OK-SIS only formed a thin and loose epithelium. Whereas the model cultured with OK-SIS-ADSC was much thicker and denser. The co-cultured of ADSC and OK grew well on the SIS in which the OKs formed a multilayer of epithelium. So it is feasible to construct a tissue-engineered oral mucosa graft with ADSCs, OKs and SIS. The ADSCs contribute to a thicker epithelium formation.

Mesenchymal stem cells participate in oral mucosa carcinogenesis by regulating T cell proliferation

2019 ◽  
Vol 198 ◽  
pp. 46-53 ◽  
Author(s):  
Yichen Chen ◽  
Xi Wang ◽  
Juan Fang ◽  
Jingjing Song ◽  
Da Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
T Cell ◽  
Oral Mucosa ◽  
T Cell Proliferation

scholarly journals Wharton's Jelly Stem Cells: A Novel Cell Source for Oral Mucosa and Skin Epithelia Regeneration

2013 ◽  
Vol 2 (8) ◽  
pp. 625-632 ◽  
Author(s):  
Ingrid Garzón ◽  
Juliano Miyake ◽  
Miguel González-Andrades ◽  
Ramón Carmona ◽  
Carmen Carda ◽  
...  
Keyword(s):  
Stem Cells ◽  
Oral Mucosa ◽  
Wharton’S Jelly ◽  
Wharton's Jelly ◽  
Cell Source

scholarly journals Can Human Oral Mucosa Stem Cells Differentiate to Corneal Epithelia?

2021 ◽  
Vol 22 (11) ◽  
pp. 5976
Author(s):  
Sonia López ◽  
Lía Hoz ◽  
Eda Patricia Tenorio ◽  
Beatriz Buentello ◽  
Fátima Sofía Magaña ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Oral Mucosa ◽  
Amniotic Membrane ◽  
Embryonic Stem ◽  
Human Amniotic Membrane ◽  
Anatomical Location ◽  
Multilineage Differentiation ◽  
Therapeutic Alternative ◽  
E Cadherin

Human oral mucosa stem cells (hOMSCs) arise from the neural crest, they can self-renew, proliferate, and differentiate to several cell lines and could represent a good source for application in tissue engineering. Because of their anatomical location, hOMSCs are easy to isolate, have multilineage differentiation capacity and express embryonic stem cells markers such as—Sox2, Oct3/4 and Nanog. We have used SHEM (supplemented hormonal epithelial medium) media and cultured hOMSCs over human amniotic membrane and determined the cell’s capacity to differentiate to an epithelial-like phenotype and to express corneal specific epithelial markers—CK3, CK12, CK19, Pan-cadherin and E-cadherin. Our results showed that hOMSCs possess the capacity to attach to the amniotic membrane and express CK3, CK19, Pan-Cadherin and E-Cadherin without induction with SHEM media and expressed CK12 or changed the expression pattern of E-Cadherin to a punctual-like feature when treated with SHEM media. The results observed in this study show that hOMSCs possess the potential to differentiate toward epithelial cells. In conclusion, our results revealed that hOMSCs readily express markers for corneal determination and could provide the ophthalmology field with a therapeutic alternative for tissue engineering to achieve corneal replacement when compared with other techniques. Nevertheless, further studies are needed to develop a predictable therapeutic alternative for cornea replacement.

scholarly journals Carboxy-Terminal Cementum Protein 1-Derived Peptide 4 (cemp1-p4) Promotes Mineralization through wnt/β-catenin Signaling in Human Oral Mucosa Stem Cells

2020 ◽  
Vol 21 (4) ◽  
pp. 1307
Author(s):  
Rita Arroyo ◽  
Sonia López ◽  
Enrique Romo ◽  
Gonzalo Montoya ◽  
Lía Hoz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Secondary Structure ◽  
Oral Mucosa ◽  
Glycogen Synthase ◽  
Biological Activities ◽  
Direct Role ◽  
Alp Activity ◽  
Coiled Structure

Human cementum protein 1 (CEMP1) is known to induce cementoblast and osteoblast differentiation and alkaline phosphatase (ALP) activity in human periodontal ligament-derived cells in vitro and promotes bone regeneration in vivo. CEMP1′s secondary structure analysis shows that it has a random-coiled structure and is considered an Intrinsic Disordered Protein (IDP). CEMP1′s short peptide sequences mimic the biological capabilities of CEMP1. However, the role and mechanisms of CEMP1′s C-terminal-derived synthetic peptide (CEMP1-p4) in the canonical Wnt/β-catenin signaling pathway are yet to be described. Here we report that CEMP1-p4 promotes proliferation and differentiation of Human Oral Mucosa Stem Cells (HOMSCs) by activating the Wnt/β-catenin pathway. CEMP1-p4 stimulation upregulated the expression of β-catenin and glycogen synthase kinase 3 beta (GSK-3B) and activated the transcription factors TCF1/7 and Lymphoid Enhancer binding Factor 1 (LEF1) at the mRNA and protein levels. We found translocation of β-catenin to the nucleus in CEMP1-p4-treated cultures. The peptide also penetrates the cell membrane and aggregates around the cell nucleus. Analysis of CEMP1-p4 secondary structure revealed that it has a random-coiled structure. Its biological activities included the induction to nucleate hydroxyapatite crystals. In CEMP1-p4-treated HOMSCs, ALP activity and calcium deposits increased. Expression of Osterix (OSX), Runt-related transcription factor 2 (RUNX2), Integrin binding sialoproptein (IBSP) and osteocalcin (OCN) were upregulated. Altogether, these data show that CEMP1-p4 plays a direct role in the differentiation of HOMSCs to a “mineralizing-like” phenotype by activating the β-catenin signaling cascade.

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