Piezo-Electrically Driven Mechanical Stimulation of Sensory Neurons

2013 ◽  
pp. 159-170 ◽  
Author(s):  
Jizhe Hao ◽  
Jérôme Ruel ◽  
Bertrand Coste ◽  
Yann Roudaut ◽  
Marcel Crest ◽  
...  
Keyword(s):  
Sensory Neurons ◽  
Mechanical Stimulation ◽  
Stimulation Of

Activity-Dependent Sensitivity of Proprioceptive Sensory Neurons in the Stick Insect Femoral Chordotonal Organ

2002 ◽  
Vol 88 (5) ◽  
pp. 2387-2398 ◽  
Author(s):  
Ralph A. DiCaprio ◽  
Harald Wolf ◽  
Ansgar Büschges
Keyword(s):  
Sensory Neurons ◽  
Mechanical Stimulation ◽  
Stick Insect ◽  
Chordotonal Organ ◽  
Afferent Neurons ◽  
Generator Potential ◽  
Slow Adaptation ◽  
Wide Range ◽  
Mechanical Factors ◽  
Stimulation Of

Mechanosensory neurons exhibit a wide range of dynamic changes in response, including rapid and slow adaptation. In addition to mechanical factors, electrical processes may also contribute to sensory adaptation. We have investigated adaptation of afferent neurons in the stick insect femoral chordotonal organ (fCO). The fCO contains sensory neurons that respond to position, velocity, and acceleration of the tibia. We describe the influence of random mechanical stimulation of the fCO on the response of fCO afferent neurons. The activity of individual sensory neurons was recorded intracellularly from their axons in the main leg nerve. Most fCO afferents (93%) exhibited a marked decrease in response to trapezoidal stimuli following sustained white noise stimulation (bandwidth = 60 Hz, amplitudes from ±5 to ±30°). Concurrent decreases in the synaptic drive to leg motoneurons and interneurons were also observed. Electrical stimulation of spike activity in individual fCO afferents in the absence of mechanical stimulation also led to a dramatic decrease in response in 15 of 19 afferents tested. This indicated that electrical processes are involved in the regulation of the generator potential or encoding of action potentials and partially responsible for the decreased response of the afferents. Replacing Ca2+ with Ba2+ in the saline surrounding the fCO greatly reduced or blocked the decrease in response elicited by electrically induced activity or mechanical stimulation when compared with control responses. Our results indicate that activity of fCO sensory neurons strongly affects their sensitivity, most likely via Ca2+-dependent processes.

Mechanical stimulation of human BON cells: Gαq involvement in 5-HT release

2001 ◽  
Vol 120 (5) ◽  
pp. A83-A83
Author(s):  
M KIM ◽  
N JAVED ◽  
F CHRISTOFI ◽  
H COOKE
Keyword(s):  
Mechanical Stimulation ◽  
Bon Cells ◽  
Stimulation Of

Use of Flexible Materials with a Novel Pressure Driven Equibiaxial Cell Stretching Device for Mechanical Stimulation of Single Mammalian Cells

2003 ◽  
Vol 773 ◽  
Author(s):  
James D. Kubicek ◽  
Stephanie Brelsford ◽  
Philip R. LeDuc
Keyword(s):  
Cell Fate ◽  
Mechanical Stimulation ◽  
Mammalian Cells ◽  
Cellular Response ◽  
Single Cells ◽  
Complex Nature ◽  
Cellular Behavior ◽  
Cell Stretching ◽  
Stimulation Of ◽  
Pressure Driven

AbstractMechanical stimulation of single cells has been shown to affect cellular behavior from the molecular scale to ultimate cell fate including apoptosis and proliferation. In this, the ability to control the spatiotemporal application of force on cells through their extracellular matrix connections is critical to understand the cellular response of mechanotransduction. Here, we develop and utilize a novel pressure-driven equibiaxial cell stretching device (PECS) combined with an elastomeric material to control specifically the mechanical stimulation on single cells. Cells were cultured on silicone membranes coated with molecular matrices and then a uniform pressure was introduced to the opposite surface of the membrane to stretch single cells equibiaxially. This allowed us to apply mechanical deformation to investigate the complex nature of cell shape and structure. These results will enhance our knowledge of cellular and molecular function as well as provide insights into fields including biomechanics, tissue engineering, and drug discovery.

Mechanical stimulation of duck on rice phyto-morphology in rice-duck farming system

2012 ◽  
Vol 20 (6) ◽  
pp. 717-722 ◽  
Author(s):  
Zhao-Xiang HUANG ◽  
Jia-En ZHANG ◽  
Kai-Ming LIANG ◽  
Guo-Ming QUAN ◽  
Ben-Liang ZHAO
Keyword(s):  
Mechanical Stimulation ◽  
Farming System ◽  
Stimulation Of

A microfluidic device for chemical and mechanical stimulation of mesenchymal stem cells

2011 ◽  
Vol 11 (5) ◽  
pp. 545-556 ◽  
Author(s):  
Huei-Wen Wu ◽  
Chun-Che Lin ◽  
Shiaw-Min Hwang ◽  
Yu-Jen Chang ◽  
Gwo-Bin Lee
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Microfluidic Device ◽  
Mechanical Stimulation ◽  
Stimulation Of

scholarly journals A Novel Bioreactor for the Mechanical Stimulation of Clinically Relevant Scaffolds for Muscle Tissue Engineering Purposes

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 474
Author(s):  
Silvia Todros ◽  
Silvia Spadoni ◽  
Edoardo Maghin ◽  
Martina Piccoli ◽  
Piero G. Pavan
Keyword(s):  
Mechanical Stimulation ◽  
Strain Range ◽  
Tensile Tests ◽  
Muscular Tissue ◽  
Fe Model ◽  
Mechanical Stimuli ◽  
Physiological Strain ◽  
Cellular Alignment ◽  
Stimulation Of

Muscular tissue regeneration may be enhanced in vitro by means of mechanical stimulation, inducing cellular alignment and the growth of functional fibers. In this work, a novel bioreactor is designed for the radial stimulation of porcine-derived diaphragmatic scaffolds aiming at the development of clinically relevant tissue patches. A Finite Element (FE) model of the bioreactor membrane is developed, considering two different methods for gripping muscular tissue patch during the stimulation, i.e., suturing and clamping with pliers. Tensile tests are carried out on fresh and decellularized samples of porcine diaphragmatic tissue, and a fiber-reinforced hyperelastic constitutive model is assumed to describe the mechanical behavior of tissue patches. Numerical analyses are carried out by applying pressure to the bioreactor membrane and evaluating tissue strain during the stimulation phase. The bioreactor designed in this work allows one to mechanically stimulate tissue patches in a radial direction by uniformly applying up to 30% strain. This can be achieved by adopting pliers for tissue clamping. Contrarily, the use of sutures is not advisable, since high strain levels are reached in suturing points, exceeding the physiological strain range and possibly leading to tissue laceration. FE analysis allows the optimization of the bioreactor configuration in order to ensure an efficient transduction of mechanical stimuli while preventing tissue damage.

scholarly journals STUDIES WITH THE ELECTROCARDIOGRAPH ON THE ACTION OF THE VAGUS NERVE ON THE HUMAN HEART

1911 ◽  
Vol 14 (3) ◽  
pp. 217-234 ◽  
Author(s):  
G. Canby Robinson ◽  
George Draper
Keyword(s):  
Vagus Nerve ◽  
Mechanical Stimulation ◽  
Human Heart ◽  
Appreciable Effect ◽  
Vagus Stimulation ◽  
Ventricular Systole ◽  
Constant Change ◽  
The Right ◽  
Stimulation Of

In hearts showing auricular fibrillation mechanical stimulation of the right vagus nerve causes, as a rule, marked slowing or stoppage of ventricular rhythm, without producing any appreciable effect in the electrocardiographic record of the auricular fibrillation. The ventricular pauses are apparently due to the blocking of stimuli from the auricles. The force of ventricular systole is distinctly weakened for several beats after vagus stimulation, and ectopic ventricular systoles have been seen in several instances, apparently the result of the vagus action. There may, in some cases, be lowered excitability of the ventricles, while no constant change is seen in the size of the electrical complexes representing ventricular systole.

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