Genetic and Molecular Analyses of UniformMu Transposon Insertion Lines

Nodosilinea epilithica Perkerson et Casamatta is reported for the first time for Russia. The sample was isolated from a typical chestnut soil in the zone of dry steppes (Volgograd Region) and its identity was confirmed by morphological and molecular analyses. The specific feature of the studied strain is its ability to form nodules at normal (60–75 μmol photons ∙ m-2 ∙ sec-1) light. The number of nodules is supposed to be related to the age of a cyanobacterial culture.

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Faculty Opinions recommendation of Identification of a functional transposon insertion in the maize domestication gene tb1.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13357167.14765060 ◽

2011 ◽

Author(s):

Miltos Tsiantis

Keyword(s):

Transposon Insertion ◽

Maize Domestication

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Faculty Opinions recommendation of Transposon-insertion sequencing screens unveil requirements for EHEC growth and intestinal colonization.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.736413515.793564769 ◽

2019 ◽

Author(s):

Martin Marinus

Keyword(s):

Intestinal Colonization ◽

Transposon Insertion ◽

Transposon Insertion Sequencing

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Rubiacearum Americanarum Magna Hama Pars XLV: More New Species and Taxonomic Changes in Palicourea (Rubiaceae, Palicoureeae) and Psychotria subg. Heteropsychotria

Novon A Journal for Botanical Nomenclature ◽

10.3417/2019387 ◽

2019 ◽

Vol 27 (3) ◽

pp. 165-195

Author(s):

Charlotte M. Taylor

Keyword(s):

New Species ◽

Molecular Analyses ◽

The Andes ◽

Taxonomic Changes ◽

Species Groups ◽

Three New Species ◽

Taxonomic Studies

Psychotria subg. Heteropsychotria Steyerm. has been shown by morphological and molecular analyses to be polyphyletic. Most of its species, including its type, belong to Palicourea Aubl. (“Pal.”). Thirty-two species of this group are reviewed here, and 17 species in Psychotria L. are transferred to Palicourea and one to Rudgea Salisb. Two replacement names, Pal. agudeloana C. M. Taylor and Pal. tabayensis C. M. Taylor, are published. Taxonomic studies here clarify circumscriptions of similar, often-confused species for several distinctive species groups found variously in Mesoamerica and the Andes: the Palicourea aschersonianoides group, the Palicourea galeottiana group, the Palicourea sulphurea group, and the Palicourea tristis group. Three new species of Palicourea are described: Pal. aschersonianula C. M. Taylor, Pal. gonzaleziana C. M. Taylor, and Pal. wachterae C. M. Taylor. Nineteen names are newly typified, and infrageneric classifications are noted for the species of Palicourea studied.

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Escalation of Pyrethroid Resistance in the Malaria Vector Anopheles funestus Induces a Loss of Efficacy of Piperonyl Butoxide–Based Insecticide-Treated Nets in Mozambique

The Journal of Infectious Diseases ◽

10.1093/infdis/jiz139 ◽

2019 ◽

Vol 220 (3) ◽

pp. 467-475 ◽

Cited By ~ 16

Author(s):

Jacob M Riveron ◽

Silvie Huijben ◽

Williams Tchapga ◽

Magellan Tchouakui ◽

Murielle J Wondji ◽

...

Keyword(s):

Cytochrome P450 ◽

Pyrethroid Resistance ◽

Anopheles Funestus ◽

Resistance Mechanisms ◽

Piperonyl Butoxide ◽

Insecticide Treated Nets ◽

Molecular Analyses ◽

Cytochrome P450 Genes ◽

High Degree ◽

Long Lasting Insecticidal Nets

Abstract Background Insecticide resistance poses a serious threat to insecticide-based interventions in Africa. There is a fear that resistance escalation could jeopardize malaria control efforts. Monitoring of cases of aggravation of resistance intensity and its impact on the efficacy of control tools is crucial to predict consequences of resistance. Methods The resistance levels of an Anopheles funestus population from Palmeira, southern Mozambique, were characterized and their impact on the efficacy of various insecticide-treated nets established. Results A dramatic loss of efficacy of all long-lasting insecticidal nets (LLINs), including piperonyl butoxide (PBO)–based nets (Olyset Plus), was observed. This An. funestus population consistently (2016, 2017, and 2018) exhibited a high degree of pyrethroid resistance. Molecular analyses revealed that this resistance escalation was associated with a massive overexpression of the duplicated cytochrome P450 genes CYP6P9a and CYP6P9b, and also the fixation of the resistance CYP6P9a_R allele in this population in 2016 (100%) in contrast to 2002 (5%). However, the low recovery of susceptibility after PBO synergist assay suggests that other resistance mechanisms could be involved. Conclusions The loss of efficacy of pyrethroid-based LLINs with and without PBO is a concern for the effectiveness of insecticide-based interventions, and action should be taken to prevent the spread of such super-resistance.

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Identification of a Mutant Locus by Noncomplementation of a Transposon Insertion Library in Saccharomyces cerevisiae

Genetics ◽

10.1093/genetics/158.4.1825 ◽

2001 ◽

Vol 158 (4) ◽

pp. 1825-1827 ◽

Cited By ~ 1

Author(s):

Heather A Wiatrowski ◽

Marian Carlson

Keyword(s):

Saccharomyces Cerevisiae ◽

New Approach ◽

Transposon Insertion ◽

Plasmid Library ◽

Mutant Locus

Abstract We describe a new approach for identifying the gene corresponding to a mutation in Saccharomyces cerevisiae. A library of mTn-lacZ/LEU2 insertions is tested for failure to complement the mutation, and the noncomplementing insertion is used to obtain sequence. This approach offers an alternative to cloning by complementation with a plasmid library.

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Discovery of the freshwater sponge genus Corvospongilla Annandale (Porifera: Spongillida) in Australia with the description of a new species and phylogeographic implications

Limnology ◽

10.1007/s10201-021-00670-2 ◽

2021 ◽

Author(s):

Renata Manconi ◽

Dirk Erpenbeck ◽

Jane Fromont ◽

Gert Wörheide ◽

Roberto Pronzato

Keyword(s):

New Species ◽

Morphological Traits ◽

Freshwater Sponge ◽

Freshwater Sponges ◽

Molecular Analyses ◽

Biogeographic Patterns ◽

Hydrographic Basin ◽

Integrative Systematics ◽

North Western ◽

A New Species

AbstractA recent discovery of freshwater sponges in an unexplored hydrographic basin in north-western Australia provided the opportunity to investigate the genus Corvospongilla Annandale (Spongillida: Spongillidae) using integrative systematics. Emendation of the genus diagnosis is provided. A comparative analysis of a Corvospongilla global dataset of morphological traits together with biogeographic patterns disclosed a new Australasian Corvospongilla species and along with molecular analyses provided the basis for a phylogenetic and phylogeographic tree for some Asian, Afrotropical and Australasian lineages.

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