Molecular analyses of the disjunctly distributed Osmunda regalis and O. japonica (Osmundaceae), with particular reference to introgression and hybridization

2021 ◽  
Vol 307 (3) ◽  
Author(s):  
Chie Tsutsumi ◽  
Yoko Yatabe-Kakugawa ◽  
Yumiko Hirayama ◽  
Wen-Liang Chiou ◽  
Masahiro Kato
Keyword(s):  
Molecular Analyses

A polyphasic approach (metabolomics, morphological and molecular analyses) in the systematics of Cladobotryum species in Greece

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
N Milic ◽  
S Kostidis ◽  
A Stavrou ◽  
Z Gonou-Zagou ◽  
VN Kouvelis ◽  
...  
Keyword(s):  
Polyphasic Approach ◽  
Molecular Analyses

First record of the soil cyanobacterium Nodosilinea epilithica (Synechococcales, Cyanobacteria) in Russia

2016 ◽  
Vol 50 ◽  
pp. 125-141
Author(s):  
A. D. Temraleeva ◽  
S. A. Dronova
Keyword(s):  
Chestnut Soil ◽  
First Record ◽  
Molecular Analyses ◽  
Dry Steppes ◽  
Cyanobacterial Culture ◽  
Volgograd Region ◽  
First Time

Nodosilinea epilithica Perkerson et Casamatta is reported for the first time for Russia. The sample was isolated from a typical chestnut soil in the zone of dry steppes (Volgograd Region) and its identity was confirmed by morphological and molecular analyses. The specific feature of the studied strain is its ability to form nodules at normal (60–75 μmol photons ∙ m-2 ∙ sec-1) light. The number of nodules is supposed to be related to the age of a cyanobacterial culture.

Rubiacearum Americanarum Magna Hama Pars XLV: More New Species and Taxonomic Changes in Palicourea (Rubiaceae, Palicoureeae) and Psychotria subg. Heteropsychotria

2019 ◽  
Vol 27 (3) ◽  
pp. 165-195
Author(s):  
Charlotte M. Taylor
Keyword(s):  
New Species ◽  
Molecular Analyses ◽  
The Andes ◽  
Taxonomic Changes ◽  
Species Groups ◽  
Three New Species ◽  
Taxonomic Studies

Psychotria subg. Heteropsychotria Steyerm. has been shown by morphological and molecular analyses to be polyphyletic. Most of its species, including its type, belong to Palicourea Aubl. (“Pal.”). Thirty-two species of this group are reviewed here, and 17 species in Psychotria L. are transferred to Palicourea and one to Rudgea Salisb. Two replacement names, Pal. agudeloana C. M. Taylor and Pal. tabayensis C. M. Taylor, are published. Taxonomic studies here clarify circumscriptions of similar, often-confused species for several distinctive species groups found variously in Mesoamerica and the Andes: the Palicourea aschersonianoides group, the Palicourea galeottiana group, the Palicourea sulphurea group, and the Palicourea tristis group. Three new species of Palicourea are described: Pal. aschersonianula C. M. Taylor, Pal. gonzaleziana C. M. Taylor, and Pal. wachterae C. M. Taylor. Nineteen names are newly typified, and infrageneric classifications are noted for the species of Palicourea studied.

scholarly journals Escalation of Pyrethroid Resistance in the Malaria Vector Anopheles funestus Induces a Loss of Efficacy of Piperonyl Butoxide–Based Insecticide-Treated Nets in Mozambique

2019 ◽  
Vol 220 (3) ◽  
pp. 467-475 ◽  
Author(s):  
Jacob M Riveron ◽  
Silvie Huijben ◽  
Williams Tchapga ◽  
Magellan Tchouakui ◽  
Murielle J Wondji ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Pyrethroid Resistance ◽  
Anopheles Funestus ◽  
Resistance Mechanisms ◽  
Piperonyl Butoxide ◽  
Insecticide Treated Nets ◽  
Molecular Analyses ◽  
Cytochrome P450 Genes ◽  
High Degree ◽  
Long Lasting Insecticidal Nets

Abstract Background Insecticide resistance poses a serious threat to insecticide-based interventions in Africa. There is a fear that resistance escalation could jeopardize malaria control efforts. Monitoring of cases of aggravation of resistance intensity and its impact on the efficacy of control tools is crucial to predict consequences of resistance. Methods The resistance levels of an Anopheles funestus population from Palmeira, southern Mozambique, were characterized and their impact on the efficacy of various insecticide-treated nets established. Results A dramatic loss of efficacy of all long-lasting insecticidal nets (LLINs), including piperonyl butoxide (PBO)–based nets (Olyset Plus), was observed. This An. funestus population consistently (2016, 2017, and 2018) exhibited a high degree of pyrethroid resistance. Molecular analyses revealed that this resistance escalation was associated with a massive overexpression of the duplicated cytochrome P450 genes CYP6P9a and CYP6P9b, and also the fixation of the resistance CYP6P9a_R allele in this population in 2016 (100%) in contrast to 2002 (5%). However, the low recovery of susceptibility after PBO synergist assay suggests that other resistance mechanisms could be involved. Conclusions The loss of efficacy of pyrethroid-based LLINs with and without PBO is a concern for the effectiveness of insecticide-based interventions, and action should be taken to prevent the spread of such super-resistance.

scholarly journals Discovery of the freshwater sponge genus Corvospongilla Annandale (Porifera: Spongillida) in Australia with the description of a new species and phylogeographic implications

Limnology ◽  
2021 ◽  
Author(s):  
Renata Manconi ◽  
Dirk Erpenbeck ◽  
Jane Fromont ◽  
Gert Wörheide ◽  
Roberto Pronzato
Keyword(s):  
New Species ◽  
Morphological Traits ◽  
Freshwater Sponge ◽  
Freshwater Sponges ◽  
Molecular Analyses ◽  
Biogeographic Patterns ◽  
Hydrographic Basin ◽  
Integrative Systematics ◽  
North Western ◽  
A New Species

AbstractA recent discovery of freshwater sponges in an unexplored hydrographic basin in north-western Australia provided the opportunity to investigate the genus Corvospongilla Annandale (Spongillida: Spongillidae) using integrative systematics. Emendation of the genus diagnosis is provided. A comparative analysis of a Corvospongilla global dataset of morphological traits together with biogeographic patterns disclosed a new Australasian Corvospongilla species and along with molecular analyses provided the basis for a phylogenetic and phylogeographic tree for some Asian, Afrotropical and Australasian lineages.

Molecular analysis confirms Laurenciella marilzae (Rhodophyta, Rhodomelaceae) in the Mediterranean Sea, a species often misidentified as Laurencia dendroidea

2020 ◽  
Vol 63 (6) ◽  
pp. 527-535
Author(s):  
Donatella Serio ◽  
Giovanni Furnari ◽  
Yola Metti
Keyword(s):  
Mediterranean Sea ◽  
Molecular Analysis ◽  
Molecular Data ◽  
Integrative Approach ◽  
Molecular Analyses ◽  
The Mediterranean ◽  
Laurencia Majuscula

AbstractIt was noted that Mediterranean specimens collected at different stations from around Sicily, Italy and referred to as Laurencia dendroidea (as Laurencia majuscula) were similar to the recently described species Laurenciella marilzae. Presented in this study are the results of an integrative approach using both morphology and molecular data (COI-5P + rbcL) to establish which taxon these specimens should be referred to. Molecular analyses show these specimens belong to Laurenciella, and strongly suggest they are within the species L. marilzae. Morphological examinations of these Mediterranean specimens were also detailed and found to support the conclusion that they belong to L. marilzae.

scholarly journals Mutant p53L194F Harboring Luminal-A Breast Cancer Cells Are Refractory to Apoptosis and Cell Cycle Arrest in Response to MortaparibPlus, a Multimodal Small Molecule Inhibitor

Cancers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 3043
Author(s):  
Ahmed Elwakeel ◽  
Anissa Nofita Sari ◽  
Jaspreet Kaur Dhanjal ◽  
Hazna Noor Meidinna ◽  
Durai Sundar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Cancer Cells ◽  
Small Molecule ◽  
Breast Cancer Cells ◽  
Luminal A ◽  
Molecular Analyses ◽  
Cycle Arrest ◽  
Mcf 7

We previously performed a drug screening to identify a potential inhibitor of mortalin–p53 interaction. In four rounds of screenings based on the shift in mortalin immunostaining pattern from perinuclear to pan-cytoplasmic and nuclear enrichment of p53, we had identified MortaparibPlus (4-[(1E)-2-(2-phenylindol-3-yl)-1-azavinyl]-1,2,4-triazole) as a novel synthetic small molecule. In order to validate its activity and mechanism of action, we recruited Luminal-A breast cancer cells, MCF-7 (p53wild type) and T47D (p53L194F) and performed extensive biochemical and immunocytochemical analyses. Molecular analyses revealed that MortaparibPlus is capable of abrogating mortalin–p53 interaction in both MCF-7 and T47D cells. Intriguingly, upregulation of transcriptional activation function of p53 (as marked by upregulation of the p53 effector gene—p21WAF1—responsible for cell cycle arrest and apoptosis) was recorded only in MortaparibPlus-treated MCF-7 cells. On the other hand, MortaparibPlus-treated T47D cells exhibited hyperactivation of PARP1 (accumulation of PAR polymer and decrease in ATP levels) as a possible non-p53 tumor suppression program. However, these cells did not show full signs of either apoptosis or PAR-Thanatos. Molecular analyses attributed such a response to the inability of MortaparibPlus to disrupt the AIF–mortalin complexes; hence, AIF did not translocate to the nucleus to induce chromatinolysis and DNA degradation. These data suggested that the cancer cells possessing enriched levels of such complexes may not respond to MortaparibPlus. Taken together, we report the multimodal anticancer potential of MortaparibPlus that warrants further attention in laboratory and clinical studies.

scholarly journals The Radioiodination of Ribopolymers for Use in Hybridizational and Molecular Analyses

1974 ◽  
Vol 249 (7) ◽  
pp. 2143-2150
Author(s):  
Neal H. Scherberg ◽  
Samuel Refetoff
Keyword(s):  
Molecular Analyses

Update of seagrass cover and species diversity in Southern Viet Nam by remote sensing data and molecular analyses

2021 ◽  
pp. 101803
Author(s):  
Xuan-Vy Nguyen ◽  
Va-Khin Lau ◽  
Nhu-Thuy Nguyen-Nhat ◽  
Trung-Hieu Nguyen ◽  
Kim-Hoang Phan ◽  
...  
Keyword(s):  
Remote Sensing ◽  
Species Diversity ◽  
Remote Sensing Data ◽  
Viet Nam ◽  
Molecular Analyses ◽  
Sensing Data ◽  
Seagrass Cover
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