Forward Genetics Screening of Medicago truncatula Tnt1 Insertion Lines

2013 ◽  
pp. 93-100 ◽  
Author(s):  
Juan Carlos Serrani Yarce ◽  
Hee-Kyung Lee ◽  
Million Tadege ◽  
Pascal Ratet ◽  
Kirankumar S. Mysore
Keyword(s):  
Medicago Truncatula ◽  
Forward Genetics ◽  
Genetics Screening

scholarly journals FORWARD AND UNBIASED RNA-INTERFERENCE SCREEN

2021 ◽  
Author(s):  
Wadim J Kapulkin
Keyword(s):  
Rna Interference ◽  
Caenorhabditis Elegans ◽  
Reverse Genetics ◽  
Screening Strategy ◽  
Rnai Screen ◽  
Forward Genetics ◽  
Genome Wide ◽  
Complementary Resource ◽  
Genetics Screening ◽  
Caenorhabditis Elegans Genome

RNA-interference (Fire et al. 1998) is a popular ‘reverse-genetics’ screening strategy applied in Caenorhabditis elegans. Genome-wide RNAi screens are presently carried using RNAi feeding libraries. Here, we report on a complementary resource facilitating an approach to RNAi screen relying on an unbiased ‘forward-genetics’ strategy. We conclude the forward RNA interference screening is useful and feasible, with the strong expectation the presented screening mode will complement and extend on the existing, currently available, genome-wide RNAi resources.

scholarly journals Large-scale forward genetics screening identifies Trpa1 as a chemosensor for predator odor-evoked innate fear behaviors

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Yibing Wang ◽  
Liqin Cao ◽  
Chia-Ying Lee ◽  
Tomohiko Matsuo ◽  
Kejia Wu ◽  
...  
Keyword(s):  
Large Scale ◽  
Forward Genetics ◽  
Predator Odor ◽  
Genetics Screening ◽  
Innate Fear

scholarly journals A novel gene-diet interaction promotes organismal lifespan and host protection during infection via the mitochondrial UPR

PLoS Genetics ◽  
2020 ◽  
Vol 16 (12) ◽  
pp. e1009234
Author(s):  
Mustafi Raisa Amin ◽  
Siraje Arif Mahmud ◽  
Jonathan L. Dowgielewicz ◽  
Madhab Sapkota ◽  
Mark W. Pellegrino
Keyword(s):  
Vitamin B12 ◽  
Ribosomal Gene ◽  
Forward Genetics ◽  
Dependent Manner ◽  
Unfolded Protein ◽  
Host Protection ◽  
Novel Gene ◽  
Extended Lifespan ◽  
Protein Response ◽  
Genetics Screening

Cells use a variety of mechanisms to maintain optimal mitochondrial function including the mitochondrial unfolded protein response (UPRmt). The UPRmt mitigates mitochondrial dysfunction by differentially regulating mitoprotective gene expression through the transcription factor ATFS-1. Since UPRmt activation is commensurate with organismal benefits such as extended lifespan and host protection during infection, we sought to identify pathways that promote its stimulation. Using unbiased forward genetics screening, we isolated novel mutant alleles that could activate the UPRmt. Interestingly, we identified one reduction of function mutant allele (osa3) in the mitochondrial ribosomal gene mrpl-2 that activated the UPRmt in a diet-dependent manner. We find that mrpl-2(osa3) mutants lived longer and survived better during pathogen infection depending on the diet they were fed. A diet containing low levels of vitamin B12 could activate the UPRmt in mrpl-2(osa3) animals. Also, we find that the vitamin B12-dependent enzyme methionine synthase intersects with mrpl-2(osa3) to activate the UPRmt and confer animal lifespan extension at the level of ATFS-1. Thus, we present a novel gene-diet pairing that promotes animal longevity that is mediated by the UPRmt.

scholarly journals A PCR-based forward genetics screening, using expression domain-specific markers, identifies mutants in endosperm transfer cell development

2014 ◽  
Vol 5 ◽  
Author(s):  
Luis M. Muñiz ◽  
Elisa Gómez ◽  
Virginie Guyon ◽  
Maribel López ◽  
Bouchaib Khbaya ◽  
...  
Keyword(s):  
Cell Development ◽  
Forward Genetics ◽  
Transfer Cell ◽  
Expression Domain ◽  
Domain Specific ◽  
Endosperm Transfer Cell ◽  
Genetics Screening

scholarly journals Medicago truncatula esn1 Defines a Genetic Locus Involved in Nodule Senescence and Symbiotic Nitrogen Fixation

2013 ◽  
Vol 26 (8) ◽  
pp. 893-902 ◽  
Author(s):  
Jiejun Xi ◽  
Yuhui Chen ◽  
Jin Nakashima ◽  
Suo-min Wang ◽  
Rujin Chen
Keyword(s):  
Nitrogen Fixation ◽  
Medicago Truncatula ◽  
Symbiotic Nitrogen Fixation ◽  
Genetic Locus ◽  
Forward Genetics ◽  
Host Cells ◽  
Nodule Development ◽  
Marker Genes ◽  
Symbiosome Membrane ◽  
Nodule Senescence

Symbiotic interaction between Medicago truncatula and Sinorhizobium meliloti results in the formation on the host roots of new organs, nodules, in which biological nitrogen fixation takes place. In infected cells, rhizobia enclosed in a plant-derived membrane, the symbiosome membrane, differentiate to nitrogen-fixing bacteroids. The symbiosome membrane serves as an interface for metabolite and signal exchanges between the host cells and endosymbionts. At some point during symbiosis, symbiosomes and symbiotic cells are disintegrated, resulting in nodule senescence. The regulatory mechanisms that underlie nodule senescence are not fully understood. Using a forward genetics approach, we have uncovered the early senescent nodule 1 (esn1) mutant from an M. truncatula fast neutron-induced mutant collection. Nodules on esn1 roots are spherically shaped, ineffective in nitrogen fixation, and senesce early. Atypical among fixation defective mutants isolated thus far, bacteroid differentiation and expression of nifH, Leghemoglobin, and DNF1 genes are not affected in esn1 nodules, supporting the idea that a process downstream of bacteroid differentiation and nitrogenase gene expression is affected in the esn1 mutant. Expression analysis shows that marker genes involved in senescence, macronutrient degradation, and remobilization are greatly upregulated during nodule development in the esn1 mutant, consistent with a role of ESN1 in nodule senescence and symbiotic nitrogen fixation.

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