Using MARCM to Study Drosophila Brain Development

2013 ◽  
pp. 79-96 ◽  
Author(s):  
Gudrun Viktorin
Keyword(s):  
Brain Development ◽  
Drosophila Brain

scholarly journals Drosophila Brain Development: Closing the Gap between a Macroarchitectural and Microarchitectural Approach

2009 ◽  
Vol 74 (0) ◽  
pp. 235-248 ◽  
Author(s):  
A. Cardona ◽  
S. Saalfeld ◽  
P. Tomancak ◽  
V. Hartenstein
Keyword(s):  
Brain Development ◽  
Drosophila Brain ◽  
Closing The Gap

scholarly journals Multipotent neural stem cells generate glial cells of the central complex through transit amplifying intermediate progenitors in Drosophila brain development

2011 ◽  
Vol 356 (2) ◽  
pp. 553-565 ◽  
Author(s):  
Gudrun Viktorin ◽  
Nadia Riebli ◽  
Anna Popkova ◽  
Angela Giangrande ◽  
Heinrich Reichert
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Brain Development ◽  
Glial Cells ◽  
Central Complex ◽  
Intermediate Progenitors ◽  
Drosophila Brain

scholarly journals Environmental effects on Drosophila brain development and learning

2017 ◽  
Vol 221 (1) ◽  
pp. jeb169375 ◽  
Author(s):  
Xia Wang ◽  
Amei Amei ◽  
J. Steven de Belle ◽  
Stephen P. Roberts
Keyword(s):  
Brain Development ◽  
Environmental Effects ◽  
Drosophila Brain

scholarly journals Novel roles for APC family members and Wingless/Wnt signaling during Drosophila brain development

2007 ◽  
Vol 305 (1) ◽  
pp. 358-376 ◽  
Author(s):  
Melissa A. Hayden ◽  
Kathryn Akong ◽  
Mark Peifer
Keyword(s):  
Brain Development ◽  
Wnt Signaling ◽  
Family Members ◽  
Drosophila Brain

scholarly journals Larval optic nerve and adult extra-retinal photoreceptors sequentially associate with clock neurons during Drosophila brain development

Development ◽  
2002 ◽  
Vol 129 (6) ◽  
pp. 1443-1453 ◽  
Author(s):  
Sébastien Malpel ◽  
André Klarsfeld ◽  
François Rouyer
Keyword(s):  
Optic Nerve ◽  
Visual System ◽  
Circadian Clock ◽  
Brain Development ◽  
Dendritic Morphology ◽  
Retinal Photoreceptors ◽  
Severe Reduction ◽  
Drosophila Brain ◽  
Light Input ◽  
Lateral Neurons

The visual system is one of the input pathways for light into the circadian clock of the Drosophila brain. In particular, extra-retinal visual structures have been proposed to play a role in both larval and adult circadian photoreception. We have analyzed the interactions between extra-retinal structures of the visual system and the clock neurons during brain development. We first show that the larval optic nerve, or Bolwig nerve, already contacts clock cells (the lateral neurons) in the embryonic brain. Analysis of visual system-defective genotypes showed that the absence of the afferent Bolwig nerve resulted in a severe reduction of the lateral neurons dendritic arborization, and that the inhibition of nerve activity induced alterations of the dendritic morphology. During wild-type development, the loss of a functional Bolwig nerve in the early pupa was also accompanied by remodeling of the arborization of the lateral neurons. Approximately 1.5 days later, visual fibers that came from the Hofbauer-Buchner eyelet, a putative photoreceptive organ for the adult circadian clock, were seen contacting the lateral neurons. Both types of extra-retinal photoreceptors expressed rhodopsins RH5 and RH6, as well as the norpA-encoded phospholipase C. These data strongly suggest a role for RH5 and RH6, as well as NORPA, signaling in both larval and adult extra-retinal circadian photoreception. The Hofbauer-Buchner eyelet therefore does not appear to account for the previously described norpA-independent light input to the adult clock. This supports the existence of yet uncharacterized photoreceptive structures in Drosophila.

scholarly journals Autophagy-dependent filopodial kinetics restrict synaptic partner choice during Drosophila brain wiring

2019 ◽  
Author(s):  
Ferdi Ridvan Kiral ◽  
Gerit Arne Linneweber ◽  
Svilen Veselinov Georgiev ◽  
Bassem A. Hassan ◽  
Max von Kleist ◽  
...  
Keyword(s):  
Brain Development ◽  
Building Material ◽  
Synapse Formation ◽  
Degradation Mechanism ◽  
Partner Choice ◽  
4D Imaging ◽  
Interaction Kinetics ◽  
Drosophila Brain ◽  
Autophagic Degradation ◽  
Kinetics Of

SummaryBrain wiring is remarkably precise, yet most neurons readily form synapses with incorrect partners when given the opportunity. Dynamic axon-dendritic positioning can restrict synaptogenic encounters, but the spatiotemporal interaction kinetics and their regulation remain essentially unknown inside developing brains. Here we show that the kinetics of axonal filopodia restrict synapse formation and partner choice for neurons that are not otherwise prevented from making incorrect synapses. Using 4D imaging in developing Drosophila brains, we show that filopodial kinetics are regulated by autophagy, a prevalent degradation mechanism whose role in brain development remains poorly understood. With surprising specificity, autophagosomes form in synaptogenic filopodia, followed by filopodial collapse. Altered autophagic degradation of synaptic building material quantitatively regulates synapse formation as shown by computational modeling and genetic experiments. Increased filopodial stability enables incorrect synaptic partnerships. Hence, filopodial autophagy restricts inappropriate partner choice through a process of kinetic exclusion that critically contributes to wiring specificity.

Generation of Standard Wild-Type MARCM Clones for Analysis of Drosophila Brain Development

2012 ◽  
Vol 2012 (12) ◽  
pp. pdb.prot071662-pdb.prot071662 ◽  
Author(s):  
C.-F. Kao ◽  
T. Lee
Keyword(s):  
Brain Development ◽  
Wild Type ◽  
Drosophila Brain
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