Real time PCR for detection of Chlamydophila pneumoniae in peripheral blood mononuclear cells of patients with multiple sclerosis

2007 ◽  
Vol 254 (9) ◽  
pp. 1293-1295 ◽  
Author(s):  
Rosa Sessa ◽  
Giovanna Schiavoni ◽  
Giovanna Borriello ◽  
Carlo Zagaglia ◽  
Fabiana Marinelli ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Real Time ◽  
Peripheral Blood Mononuclear Cells ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Chlamydophila Pneumoniae ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

scholarly journals Upregulated MicroRNA-155 Expression in Peripheral Blood Mononuclear Cells and Fibroblast-Like Synoviocytes in Rheumatoid Arthritis

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Li Long ◽  
Ping Yu ◽  
Yanying Liu ◽  
Shiyao Wang ◽  
Ru Li ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Real Time ◽  
Peripheral Blood Mononuclear Cells ◽  
Real Time Pcr ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Expression Profiles ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Fibroblast Like Synoviocytes

Objective. This study was to screen for the miRNAs differently expressed in peripheral blood mononuclear cells (PBMC) of RA, to further identify the expression of miR-155 in RA PBMC and fibroblast-like synoviocytes (FLS), and to evaluate the function of miR-155 in RA-FLS.Methods. Microarray was used to screen for differentially expressed miRNAs in RA PBMC. miR-155 expression in PBMC and FLS of RA were identified by real-time PCR. Enforced overexpression and downexpression of miR-155 were used to investigate the function of miR-155 in RA-FLS. Expression of IKBKE which was previously identified as the actual target of miR-155 was examined by Western blot and real-time PCR in RA-FLS.Results. miR-155 levels were increased in both PBMC and FLS of RA and could be induced by TNF-α. Upregulation of miR-155 decreased MMP-3 levels and suppressed proliferation and invasion of RA-FLS. Inverse relationship between the expressions of miR-155 and the MMPs production-related protein IKBKE was found.Conclusion. An inflammatory milieu may alter miRNA expression profiles in rheumatoid arthritis. miR-155 is upregulated in RA-FLS, and it may be a protective factor against the inflammatory effect in part by attenuating expression of IKBKE.

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