In recent years, the laboratory safety situation in Colleges and universities has improved, but the safety situation is still severe, so it is urgent to carry out relevant research. Based on the research basis of previous literature, this paper extracts the causes of laboratory accidents in Colleges and universities in the past six years, uses the grey system theory to carry out correlation analysis, sorts the correlation degree of influencing factors of laboratory safety behavior, and through empirical analysis, the organization system, safety education, professional safety, laboratory environment management, foundation safety show a decreasing relationship in the influencing factors of laboratory safety. On the basis of this conclusion, the safety management path of related laboratories is proposed: Improve the laboratory organization and management system; carry out safety education regularly; strict professional operation process; orderly laboratory environment; effective basic safety management. These path studies have positive practical significance for laboratory safety management.