Biphasic Osteochondral Restoration Techniques Using Synovial Stem Cells and Artificial Bone

Large osseous defects are difficult to treat because of deficient blood supply on the defected area. To get sufficient blood supply, we designed to establish the adenovirus simultaneously encoding both VEGF and Ang-1 (pAd-VIA) to accelerate the formation of new vessels in the process of bone defect repair. The construction of the adenovirus was performed according to the method reported by Tong-Chuan HE with a tiny modification. Three kinds of adenoviruses were acquired. They are adenovirus pAd-VIA simultaneously encoding VEGF and Ang-1, adenovirus pAd-VEGF encoding VEGF, and adenovirus pAd-Ang-1 encoding Ang-1. The adenovirus prepared in this study could successfully transfer VEGF and Ang-1 into mesenchymal stem cells(MSCs) with high efficiency. Two-gene modified artificial bone was established by use of these adenovirus. In the end, the two-gene modified artificial bone was proved to have good biocompatibility and biological function. Study reports presented here will pave the way for further exploration of vascularization in the process of large osseous defects repair.

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A Resorbable Biomaterial Shaped as a Tubular Chamber and Containing Stem Cells: A Pilot Study on Artificial Bone Regeneration

The International Journal of Artificial Organs ◽

10.1177/039139880002300508 ◽

2000 ◽

Vol 23 (5) ◽

pp. 331-337 ◽

Cited By ~ 12

Author(s):

R. Giardino ◽

N. Nicoli Aldini ◽

P. Torricelli ◽

M. Fini ◽

G. Giavaresi ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Pilot Study ◽

Iliac Crest ◽

Artificial Bone ◽

Percutaneous Aspiration ◽

Non Union ◽

Centrifugation Technique ◽

The Right ◽

New Zealand Rabbits

In a previous study, we showed how healing of non-union defects in rabbit radii can be achieved by means of a tubular resorbable chamber, in comparison with untreated defects. In the present study, we placed bone marrow stem cells inside the chamber. Bone marrow was obtained by percutaneous aspiration from the iliac crest in 9 adult New Zealand rabbits. Stem cells were separated by the centrifugation technique. In the same animals, a defect of 10 mm was created in both radii. On the left side, the defect was treated with the poly-DL-Lactide chamber, in which a suspension of autologous cells was injected; on the right side, only autologous cells were used. Radiological and histomorphometric data were compared within this study as well as with the results of our previous study. At 3, 6 and 9 months, there was no healing on the right side. On the left side, progressive bone formation with reunion of the stumps was observed in the chamber. We conclude that stem cells can accelerate bone healing when contained in the tubular chamber.

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Tailorable hierarchical structures of biomimetic hydroxyapatite micro/nano particles promoting endocytosis and osteogenic differentiation of stem cells

Biomaterials Science ◽

10.1039/d0bm00443j ◽

2020 ◽

Vol 8 (12) ◽

pp. 3286-3300 ◽

Cited By ~ 5

Author(s):

Dong Xu ◽

Yuxin Wan ◽

Zhihao Li ◽

Chunbao Wang ◽

Qingxia Zou ◽

...

Keyword(s):

Stem Cells ◽

Osteogenic Differentiation ◽

Drug Carrier ◽

Hierarchical Structures ◽

Nano Particles ◽

Great Promise ◽

Artificial Bone ◽

Biomimetic Hydroxyapatite ◽

Carrier Systems ◽

Drug Carrier Systems

Hydroxyapatite (HA) micro/nano particles show great promise as artificial bone and dental substitutes, or drug carrier systems.

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Comparison of 2 Different Formulations of Artificial Bone for a Hybrid Implant With a Tissue-Engineered Construct Derived From Synovial Mesenchymal Stem Cells: A Study Using a Rabbit Osteochondral Defect Model

The American Journal of Sports Medicine ◽

10.1177/0363546516668835 ◽

2016 ◽

Vol 45 (3) ◽

pp. 666-675 ◽

Cited By ~ 11

Author(s):

Kazunori Shimomura ◽

Yu Moriguchi ◽

Ryosuke Nansai ◽

Hiromichi Fujie ◽

Wataru Ando ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteochondral Defect ◽

Artificial Bone ◽

Defect Model

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Imaging Observation of Nano-Artificial Bone in the Repair of the Defect in Osteonecrosis of the Femoral Head

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2020.18708 ◽

2020 ◽

Vol 20 (12) ◽

pp. 7775-7780

Author(s):

Ziyan Li ◽

Xiurong Yang ◽

Shuang Liang ◽

Hongyi Li ◽

Linlin Hu ◽

...

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Mesenchymal Stem Cells ◽

Femoral Head ◽

Bone Defects ◽

Control Group ◽

Graft Material ◽

Artificial Bone ◽

Significant Difference ◽

Marrow Mesenchymal Stem Cells

To observe the effect of nano-artificial bone and bone marrow mesenchymal stem cells (BMSCs) in the treatment of femoral head osteonecrosis. The bilateral femoral head internal bone defect model was established and divided into three groups. Group A was used to make the defect without filling any material as the control, group B was only filled with nano-artificial bone, and group C was filled with composite materials of nano-artificial bone and bone marrow mesenchymal stem cells. The femoral head was examined using radiography and high-resolution focused 48-slice computed tomography (CT) at 12 weeks after implantation. A significant difference was found between groups B and C in the aspect of repairing the defect in osteogenesis of the femoral head as compared with the control group. Nano-collagen-based bone has strong osteogenic and osteogenic effects and is a good graft material for repairing bone defects of the femoral head. The use of bone marrow mesenchymal stem cells can promote the repair of bone defects, which is of great value in the treatment of osteonecrosis of the femoral head.

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Development of Cell-hybrid Artificial Bone: Effect of Osteogenic Differentiation of Bone Marrow Stromal Stem Cells on Bone Formation with Newly Developed Interconnected Porous Calcium Hydroxyapatite

Dental Materials Journal ◽

10.4012/dmj.26.162 ◽

2007 ◽

Vol 26 (2) ◽

pp. 162-169 ◽

Cited By ~ 12

Author(s):

Kazuya DOI ◽

Takayasu KUBO ◽

Kazuhiko HAYASHI ◽

Koichi IMURA ◽

Yasumasa AKAGAWA

Keyword(s):

Stem Cells ◽

Bone Marrow ◽

Bone Formation ◽

Osteogenic Differentiation ◽

Cell Hybrid ◽

Calcium Hydroxyapatite ◽

Artificial Bone ◽

Stromal Stem Cells ◽

Bone Effect

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Basic study on artificial bone-cartilage tissue construction using two layered scaffold with mesenchymal stem cells

The Proceedings of the Bioengineering Conference Annual Meeting of BED/JSME ◽

10.1299/jsmebio.2017.29.2f17 ◽

2017 ◽

Vol 2017.29 (0) ◽

pp. 2F17

Author(s):

Takaaki ARAHIRA ◽

Mitsugu TODO

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cartilage Tissue ◽

Artificial Bone ◽

Basic Study

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3D Printed Hydroxyapatite/Silk Fibroin/Polycaprolactone Artificial Bone Scaffold and Bone Tissue Engineering Materials Constructed with Double-Transfected Bone Morphogenetic Protein-2 and Vascular Endothelial Growth Factor Mesenchymal Stem Cells to Repair Rabbit Radial Bone Defects

Nanoscience and Nanotechnology Letters ◽

10.1166/nnl.2020.3112 ◽

2020 ◽

Vol 12 (3) ◽

pp. 368-375

Author(s):

Xu Li ◽

Fang Ye ◽

Guoliang Li ◽

Jun Cui ◽

Yixiu Liu ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Tissue ◽

Bone Defect ◽

Bone Defects ◽

Bone Scaffold ◽

Artificial Bone ◽

Significant Difference ◽

Post Operation ◽

Radial Bone

To examine the effect of bone tissue engineering material constructed via 3D printed nanohydroxyapatite/silk fibroin/polycaprolactone (nHA/SF/PCL) artificial bone scaffolds and doubletransfected BMP-2/VEGF mesenchymal stem cells in repairing rabbit radial bone defects, 60 New Zealand rabbits were randomly selected, and with the establishment of a 15 mm bone defect model upon the middle of the anterior right radius bone. The rabbits were randomly divided into 4 groups, with each group composed of 15 rabbits: Group A (nHA/SF/PCL BMP-2/VEGF), Group B (simple nHA/SF/PCL), Group C (simple BMP-2/VEGF), Group D (blank control). Our team recorded the eating, movement, redness, exudation, and so forth across each sample group following the operation. The bone protein expression levels were measured via western blotting following 12 weeks post-operation. Histological observation was carried performed via tissue sections. Following the operation procedure, the wound healed healthily across the 4 experimental groups, no serious infections occurred, only a few rabbits exhibited redness, exudation, pus, and other phenomena, and there was no significant difference present in each group (P > 0.05). The A group took the shortest time to recover its autonomous activity ability, followed by the B group, and the D group took the longest time to recover its autonomous activity ability. From western blotting tests, the A group possessed the highest BMP-2 and OCN expression levels, and have significant difference existed with the B, C, and D group (P < 0.05). The order of expression level of BMP-2 and OCN across the other three groups were group C > group B > group D, respectively. The results of a histological section performed at 12 weeks post-operation revealed that there was no immune response present and no adverse inflammatory reaction occurring within each experimental group. Among the rabbits, the A group exhibited an apparent plate layer new bone formation, bone trabeculae were arranged regularly, and there was no obvious space within the new bone tissue; furthermore, the repair of the bone defect was completed. The B group still exhibited some bone scaffold remaining within the implantation area, and there was a small gap between the new bone tissue, and several bone defects had not been repaired. The C group of bone defects presented only a small number of new bone formation, and bone defect repair effect was not obvious. The D group still possessed large bone defects and exhibited poor repair of bone defects. The nHA/SF/PCL composite of artificial bone scaffold and double-transfected BMP-2/VEGF mesenchymal stem cells was effective in repairing radial bone defects within rabbits, and was superior to using nHA/SF/PCL bone scaffolds alone.

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