Fish Farms Effluents for Irrigation and Fertilizer: Field and Modeling Studies

Objective: To study the incidence and seasonal dynamics of different fungi affected freshwater fishes in Lake Manzala with molecular identification of the isolated fungi. Animals: 300 Nile tilapia (Oreochromis niloticus) and 300 catfish (Clarias gariepinus). Design: Descriptive study. Procedures: Random samples of Oreochromis niloticus (O. niloticus) and Clarias gariepinus (C. gariepinus) were collected from Manzala fish farms. Clinical and postmortem examination of fish was applied. Isolation and identification of different fungi were performed by conventional methods. Furthermore, the molecular characterization of isolated fungi was carried out. Results: C. gariepinus had a higher rate of infection with different fungal species than O. niloticus. Aspergillus spp. (Aspergillus niger and Aspergillus flavus) were the most fungal isolated from the examined fishes, followed by Penicillium spp. and Candida albicans. Aspergillus spp were detected in all seasons with a higher rate in summer and spring. A. flavus, A. niger, Penicillium spp. and C.albicans isolates were amplified from both C. gariepinus and O. niloticus at the specified molecular weight using PCR. Conclusion and clinical relevance: Fungal infection affected the fish showing different external and internal lesions, all species of Aspergillus were found in all seasons with a high rate in, hot seasons, summer and spring. The Prevalence of Penicillium and C. albicans were also reported. All fungal isolates were identified on the phenotypic and molecular bases.

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Modeling Studies of Ice-Ocean Dynamics and Thermodynamics

10.21236/ada264084 ◽

1993 ◽

Author(s):

George L. Mellor

Keyword(s):

Ocean Dynamics ◽

Modeling Studies

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Synopsis of Epidemic Modeling Studies

10.21236/ada460299 ◽

2001 ◽

Author(s):

Jr Bombardt ◽

John N.

Keyword(s):

Epidemic Modeling ◽

Modeling Studies

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Fish farming in Denmark: environmental impact of regulative legislation

Water Science & Technology ◽

10.2166/wst.1995.0365 ◽

1995 ◽

Vol 31 (10) ◽

pp. 73-84 ◽

Cited By ~ 3

Author(s):

T. M. Iversen

Keyword(s):

Organic Matter ◽

Environmental Impact ◽

Freshwater Fish ◽

Aquatic Environment ◽

Action Plan ◽

Fish Farming ◽

Fish Farms ◽

Nitrogen And Phosphorus ◽

Local Impact ◽

The Impact

The main environmental problems associated with fish farming in Denmark are attributable to the dam, the “dead reach” and nutrient and organic matter discharge. The environmental regulation of fish farming in Denmark started with the Environmental Protection Act of 1974, the Statutory Order of 1985 forbidding wet feed, and the Action Plan on the Aquatic Environment of 1987. In the case of freshwater fish farms, the latter was implemented through the measures stipulated in the 1989 Statutory Order on Fish Farms. The impact of Danish legislative measures to reduce and regulate the environmental effects of freshwater fish farms can be summarized as follows: - the number of fish farms has been reduced from about 800 in 1974 to about 500 at present; - production has tripled since 1974 and has been stable since 1989; - a change from wet to dry feed has reduced the environmental impact of the farms; - the national goals of the Action Plan on the Aquatic Environment of 1987 for reducing fish farm discharges of organic matter, nitrogen and phosphorus have been fulfilled. The main remaining problems are that: - the local impact of fish farms on downstream stream quality is still much too high in about 15% of cases; - the problem of the passage of migrating invertebrates and fish is still unsolved at some farms; - the problems posed by “dead reaches” are still unsolved. It is concluded that sustainable fish farming is possible in Denmark, but with the present technology production will have to be significantly reduced.

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Environmental impact of nutrients from nordic fish farming

Water Science & Technology ◽

10.2166/wst.1995.0364 ◽

1995 ◽

Vol 31 (10) ◽

pp. 61-71 ◽

Cited By ~ 14

Author(s):

M. Enell

Keyword(s):

Major Part ◽

Fish Farming ◽

Fish Farms ◽

Feed Composition ◽

The North ◽

Comprehensive Development ◽

The Baltic ◽

Inland Water Bodies ◽

The Impact ◽

Feed Coefficient

During the last 20 years there has been an interesting development of the Nordic fish farming, with regard to the feeding and farming technology and to the increase in production quantities. During the period 1974-1994 the production increased from 15,800 to about 250,000 tonnes/year. In 1974 the major part of the production was in Denmark, and in 1994 the major part was in Norway. The nutrient impact of fish farming on surrounding sea areas is mainly a function of the feed coefficient, the feed composition and metabolic processes in the fish. The comprehensive development of the feed composition and the feeding technology has resulted in reduced load of unmetabolized nutrients from fish farms, calculated per tonne fish produced. In 1974 the mean Nordic feed coefficient was 2.08 and in 1994 the coefficient was 1.25. Feed coefficients of 1.0-1.1 are now reported for Danish and Norwegian freshwater and marine fish farms. The nitrogen (N) and phosphorus (P) content of the feed has decreased, in addition the quality of the nutrient substances in the feed has changed, especially for N. The N content has decreased from 7.8 to 6.8% during the period 1974-1994 and the content of P has decreased from 1.7 to 0.7% during the same period. This development of the feed coefficient and the feed composition has resulted in a present load from a typical Nordic fish farm of 55 kg N and 4.8 kg P/t fish produced. The figures for 1974 were 132 kg N and 31 kg P/t fish produced. The Nordic fish farming production in 1994 resulted in a load of about 13,750 t N and about 1,200 t P on the actual recipients. The load from the Swedish, Finnish and Danish fish farming operations, with the Baltic Sea and the Skagerrak as the recipients, is negligible in comparison with other pollution sources. The quantities of N and P from the fish farming are equal to 0.5% of the atmospheric deposition on the sea surface and 3% of the atmospheric P load. Norwegian, Icelandic and the Faroe Islands fish farming operations are using the North Sea and the Norwegian Sea as the recipients. However, the nutrient load from single fish farms in certain coastal and inland water bodies can be significant and must be considered in the impact assessment together with other sources.

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p-Trifluoroacetophenone Oxime Ester Derivatives: Synthesis, Antimicrobial and Cytotoxic Evaluation and Molecular Modeling Studies

Letters in Drug Design & Discovery ◽

10.2174/1570180816666181128112249 ◽

2020 ◽

Vol 17 (2) ◽

pp. 169-183 ◽

Cited By ~ 1

Author(s):

İrem Bozbey ◽

Suat Sari ◽

Emine Şalva ◽

Didem Kart ◽

Arzu Karakurt

Keyword(s):

Molecular Modeling ◽

Neuroblastoma Cell ◽

Neuroblastoma Cells ◽

Cytotoxic Agents ◽

Human Neuroblastoma Cell ◽

Human Neuroblastoma ◽

Modeling Studies ◽

Broth Microdilution Method ◽

Molecular Modeling Studies

Background: Azole antifungals are among the first-line drugs clinically used for the treatment of systemic candidiasis, a deadly type of fungal infection that threatens mostly immunecompromised and hospitalized patients. Some azole derivatives were also reported to have antiproliferative effects on cancer cells. Objective: In this study, 1-(4-trifluoromethylphenyl)-2-(1H-imidazol-1-yl)ethanone (3), its oxime (4), and a series of its novel oxime ester derivatives (5a-v) were synthesized and tested for their in vitro antimicrobial activities against certain ATCC standard strains of Candida sp. fungi and bacteria. The compounds were also tested for their cytotoxic effects against mouse fibroblast and human neuroblastoma cell lines. Molecular modeling studies were performed to provide insights into their possible mechanisms for antifungal and antibacterial actions. Methods: The compounds were synthesized by the reaction of various oximes with acyl chlorides. Antimicrobial activity of the compounds was determined according to the broth microdilution method. For the determination of cytotoxic effect, we used MTS assay. Molecular docking and QM/MM studies were performed to predict the binding mechanisms of the active compounds in the catalytic site of C. albicans CYP51 (CACYP51) and S. aureus flavohemoglobin (SAFH), the latter of which was created via homology modeling. Results: 5d, 5l, and 5t showed moderate antifungal activity against C. albicans, while 3, 5c, and 5r showed significant antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Most of the compounds showed approximately 40-50% inhibition against the human neuroblastoma cells at 100 µM. In this line, 3 was the most potent with an IC50 value of 82.18 μM followed by 5a, 5o, and 5t. 3 and 5a were highly selective to the neuroblastoma cells. Molecular modelling results supported the hypothesis that our compounds were inhibitors of CAYP51 and SAFH. Conclusion: This study supports that oxime ester derivatives may be used for the development of new antimicrobial and cytotoxic agents.

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