Cell Cycle Proteins and Retinal Degeneration: Evidences of New Potential Therapeutic Targets

The response to glomerular and tubulointerstitial cell injury in most forms of renal disease includes changes in cell number (proliferation and apoptosis) and cell size (hyerptrophy). These events typically precede and may be reponsible for the accumulation of extracellular matrix proteins that leads to a decrease in renal function. There is increasing evidence showing that positive (cyclins and cyclin-dependent kinases) and negative (cyclin-dependent kinase inhibitors) cell cycle regulatory proteins have a critical role in regulating these fundamental cellular responses to immune and nonimmune forms of injury. Data now show that altering specific cell cycle proteins affects renal cell proliferation and improves renal function. Equally exciting is the expanding body of literature showing novel biological roles for cell cycle proteins in the regulation of cell hypertrophy and apoptosis. With increasing understanding of the role for cell cyle regulatory proteins in renal disease comes the hope for potential therapeutic inverventions.

Download Full-text

Prognostic Value of Cell Cycle Proteins in Squamous Cell Carcinomas of the Oral Cavity

Journal of Molecular Biomarkers & Diagnosis ◽

10.4172/2155-9929.1000111 ◽

2011 ◽

Vol 02 (03) ◽

Author(s):

Marcilei Eliza Ca vicchioli Buim ◽

José Humberto Fregnani ◽

Silvia Vanessa Lourenço ◽

Cibele Pidorodeski Nagano

Keyword(s):

Cell Cycle ◽

Oral Cavity ◽

Squamous Cell ◽

Prognostic Value ◽

Squamous Cell Carcinomas ◽

Cell Cycle Proteins

Download Full-text

Presence of Human Papillomavirus and Epstein–Barr Virus, but Absence of Merkel Cell Polyomavirus, in Head and Neck Cancer of Non-Smokers and Non-Drinkers

Frontiers in Oncology ◽

10.3389/fonc.2020.560434 ◽

2021 ◽

Vol 10 ◽

Author(s):

Frans J. Mulder ◽

Faisal Klufah ◽

Famke M. E. Janssen ◽

Farzaneh Farshadpour ◽

Stefan M. Willems ◽

...

Keyword(s):

Cell Cycle ◽

Human Papillomavirus ◽

Head And Neck ◽

Epstein Barr Virus ◽

Merkel Cell Polyomavirus ◽

Merkel Cell ◽

Cell Cycle Proteins ◽

P53 Expression ◽

Barr Virus ◽

Epstein Barr

ObjectiveDetermine the presence and prognostic value of human papillomavirus (HPV), Epstein-Barr virus (EBV), Merkel cell polyomavirus (MCPyV), and cell cycle proteins in head and neck squamous cell carcinoma (HNSCC) of non-smokers and non-drinkers (NSND).MethodsClinical characteristics and tumors of 119 NSND with HNSCC were retrospectively collected and analyzed on tissue microarrays. RNAscope in situ hybridization (ISH) was used to screen for the presence of HPV and MCPyV mRNA. Immunohistochemistry was performed for expression of p16 as surrogate marker for HPV, Large T-antigen for MCPyV, and cell cycle proteins p53 and pRb. Positive virus results were confirmed with polymerase chain reaction. For EBV, EBV encoded RNA ISH was performed. Differences in 5-year survival between virus positive and negative tumors were determined by log rank analysis.ResultsAll oropharyngeal tumors (OPSCC) (n = 10) were HPV-positive, in addition to one oral (OSCC) and one nasopharyngeal tumor (NPSCC). The other three NPSCC were EBV-positive. MCPyV was not detected. Patients with HPV or EBV positive tumors did not have a significantly better 5-year disease free or overall survival. Over 70% of virus negative OSCC showed mutant-type p53 expression.ConclusionIn this cohort, all OPSCC and NPSCC showed HPV or EBV presence. Besides one OSCC, all other oral (n = 94), hypopharyngeal (n = 1), and laryngeal (n = 9) tumors were HPV, EBV, and MCPyV negative. This argues against a central role of these viruses in the ethiopathogenesis of tumors outside the oro- and nasopharynx in NSND. So, for the majority of NSND with virus negative OSCC, more research is needed to understand the carcinogenic mechanisms in order to consider targeted therapeutic options.

Download Full-text