Bioremediation Effects of Nitrogen Fixing Trees on Nutrients and Heavy Metals in Spent Engine Oil Polluted Soil

The research aimed to investigate the bioremediation of spent engine oil on selected contaminated soils within Ilorin metropolis. To achieve this, soil samples were collected from three (3) mechanic workshops along Taiwo axis within the metropolis. The soil samples were then subjected to bioremediation using the land-farming approach. The physicochemical parameters of the soil samples before and after bioremediation were analyzed using standard methods. Bacteria were isolated using standard procedures and identified using biochemical tests and molecular methods. Results for the physicochemical parameters of the soil samples before bioremediation include particle size (all sandy in nature); pH (6.00 ± 0.14 - 6.20 ± 0.14); Organic carbon (14.65 ± 3.20 - 17.54 ± 1.87), Organic matter (33.50 ± 0.85 - 43.45 ± 9.12) and heavy metals (ND - 11.74 ± 0.07). Values after bioremediation for pH, organic carbon, organic matter and heavy metals were 8.25 ± 0.07 - 8.90 ± 0.14, 13.07 ± 0.05 - 13.25 ± 0.84, 37.25 ± 1.06 - 44.80 ± 1.13, ND - 9.40 ± 0.04 respectively. Values for bacterial count before and after bioremediation of the soil samples were 8.00 1.41 - 67.50 ± 2.12 x 105 CFU/mL and 6.50 ± 2.12 - 164.00 ± 11.31 x 105 CFU/mL respectively. Bacterial isolates were identified as Pseudomonas sp., Enterobacter sp., Acinetobacter sp., and Bacillus sp. while the hydrocarbon-utilizing bacteria were identified as Thalassospira mesophila strain JCM 18969; Pseudomonas fluorescens F113; Siccibacter turicensis LMG 23730; Pseudomonas Zeshuii strain KACC 15471; Pseudomonas stutzeri strain CGMCC 1.1803 and Marinobacter hydrocarbonoclasticus strain ATCC 49840. In conclusion, the bacteria isolates effectively bioremediated the spent engine oil contaminated soils with a reduction of hydrocarbon pollutants.

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EVALUATION OF PHYTO-EXTRACTION POTENTIALS AND PERFORMANCE OF FALSE SESAME (CERATOTHECA SESAMOIDES) UNDER INDUCED SOIL POLLUTION BY AUTOMOBILE LUBRICANT IN SAVANNA ECOREGION

International Journal of Research -GRANTHAALAYAH ◽

10.29121/granthaalayah.v5.i11.2017.2368 ◽

2017 ◽

Vol 5 (11) ◽

pp. 355-365 ◽

Cited By ~ 1

Author(s):

Babajide ◽

Popoola ◽

Gbadamosi ◽

Oyedele ◽

Liasu

Keyword(s):

Heavy Metals ◽

Heavy Metal ◽

Crop Production ◽

Metal Accumulation ◽

Heavy Metal Accumulation ◽

Engine Oil ◽

Nutrient Concentrations ◽

Polluted Soils ◽

Spent Engine Oil

While strategizing towards achieving improved soil fertility for sustainable tropical crop production, timely application of fertilizers on regular basis alone is not the University of Technology, Ogbomoso, Oyo State, Nigeria best approach, particularly on polluted soils. However, seeking for reliable natural, biological and environment friendly means of ensuring effective riddance of toxic elements or heavy metals from tropical agricultural soils is equally a worthwhile technology. An open-field potted experiment was conducted during early raining season of 2016 (March-July), at the Teaching and Research Farms of Oyo State College of Agriculture and Technology, Igboora, to investigate the effect of spent engine oil polluted soil conditions on performance and heavy metal accumulation potentials of Ceratothecasesamoides. Spent engine oil was applied at different concentrations (0.0ml, 50.0ml, 100.0ml, 150.0ml, and 200.0ml to each pot containing 7kg soil. Four pots per treatments were used. The trial was arranged in Completely Randomised Design (CRD), replicated three (3) times. Data were collected on growth parameters (number of leaves, plant height, stem girth, leaf length and leaf breadth). The data were subjected to analysis of variance (ANOVA) and Duncan Multiple Range Test (DMRT) was used to the means. Pre and post-cropping soil analyses were carried out, for determination of nutrient concentrations. Also, after the termination of the experiment, plant samples were collected from each of the pots for oven-drying followed by determination of nutrient concentrations (including the heavy metals). Oil pollution significantly affected growth and heavy metal accumulation of the test-crop. Growth decreases with increasing rate of lubricant application, while the heavy metal concentrations in the test-crop increased with increasing concentrations of the applied spent engine oil. Thus, Ceratothecasesamoides is a wild plant which could be easily exploited for its heavy metals hyper-accumulative or phyto-extractive potentials, so as to reclaim heavy metals polluted soils from toxicity, for sustainable crop production.

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Anatomical and Physiological Effects of Spent-Engine Oil on Two Varieties of Abelmoschus esculentus (L.) Moench. from Malvaceae

Notulae Scientia Biologicae ◽

10.15835/nsb10410265 ◽

2018 ◽

Vol 10 (4) ◽

pp. 584-596 ◽

Cited By ~ 1

Author(s):

Oluwaloni P. OLUWANISOLA ◽

Abdullahi A. ABDULRAHAMAN

Keyword(s):

Heavy Metals ◽

Seedling Survival ◽

Control Plant ◽

Dry Weight ◽

Nutrient Content ◽

Germination Percentage ◽

Abelmoschus Esculentus ◽

Engine Oil ◽

Human Beings ◽

Spent Engine Oil

The effect of different levels of spent engine oil application on germination, survival, growth, leaf anatomy, yield, nutrient content and heavy metals uptake of okra (Abelmoschus esculentus – ‘Clemson’ spineless variety and ‘OLA 3 Local’ variety) was assessed in the present study. Completely randomized design was used with five treatment levels of spent engine oil at 0 ml (control), 50 ml, 100 ml, 150 ml and 200 ml applied to 5 kg of soil. Data on germination, seedling survival, growth, chlorophyll nutrient and heavy metal content, as well as leaf epidermal features collected from the study were subjected to Statistical Package for Social Sciences (SPSS) analyses. Spent engine oil at 200 ml per pot significantly (P < 0.05) delayed seed germination for 4 days and reduced germination percentage by approximately 24% compared to the control. Plant height, number of leaves, leaf area, chlorophyll A, B and stomata area were reduced by 21.33-72.89%; number and dry weight of fruits were reduced by 67.4-13.58%. Number of stomata was increased on the adaxial surface by 57.73% and on the abaxial surface by 34.99%. Na, K, Cr, Cd and Fe contents increased by 0.0178-6.2698 mg/kg-1. The present study has shown that plant constituents and anatomy can be influenced by spent oil contamination. Contamination of soil with spent engine oil therefore should be avoided in order to ensure sustainable crop plant productivity and to reduce the risk of heavy metals toxicity for human beings.

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Soil Amendment Potential of Liquid and Organo-Mineral Fertilizer on Spent Engine Oil-Polluted Soil

Research Journal of Soil Biology ◽

10.3923/rjsb.2020.1.8 ◽

2019 ◽

Vol 12 (1) ◽

pp. 1-8

Author(s):

Ekeoma M. Onuoha ◽

F.A. Anukwa ◽

A. Nkang ◽

J. Nkeruwem

Keyword(s):

Soil Amendment ◽

Mineral Fertilizer ◽

Polluted Soil ◽

Engine Oil ◽

Spent Engine Oil

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Effect of Macrophomina phaseolina, organic manure and spent engine oil on Luffa aegyptica (Mill)

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v11i1.24 ◽

2018 ◽

Vol 11 (1) ◽

pp. 138-142

Author(s):

E Ani ◽

A.A. Adekunle ◽

A.B. Kadiri ◽

K.L. Njoku

Keyword(s):

Leaf Area ◽

Environmental Concern ◽

Macrophomina Phaseolina ◽

Polluted Soil ◽

Engine Oil ◽

Negative Consequences ◽

Spent Engine Oil ◽

Number Of Leaves ◽

Germination And Growth ◽

Luffa Aegyptiaca

There is an increasing environmental concern over improper disposal of spent engine oil (SEO) from serviced engines. These concerns are the results of increasing pollution of the environment by SEO with its negative consequences on plants and other forms of life. The present study assessed the effect of Macrophomina phaseolina and nutritional amendment on Luffa aegyptiaca grown on spent engine oil polluted soil in a screen house. The effect of SEO and nutritional amendment on L. aegyptiaca was assessed on germination, plant height, leaf area, stem girt and number of leaves. Two concentrations of SEO (100 and 200ml) in four kilogram of soil were used in four replications each. Results show that SEO adversely affected the germination and growth of L aegyptiaca as delayed germination, reduction in heights, leaf area and number of leaves were observed in the crop planted in SEO polluted soil. Germination (emergence) of L. aegyptiaca seeds planted in SEO polluted soil began after four days compared to two days for plants in untreated soil. Germination efficiency for seeds planted in unpolluted soil was up to 100% but reduced to 20% when the soil was polluted with SEO. However, nutritional amendment and M. phaseolina increased seed germination in SEO polluted soil from 20% to 60%. Nutritional amendment and M. phaseolina were thus shown to enhance the germination and growth of L. aegyptiaca in SEO polluted soil.Keywords: Macrophomina phaseolina, Luffa aegyptiaca, Germination, Effect, Spent engine oil

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Phytoremediation potential of Nauclea diderrichii (De Wild and Th. Dur.) seedlings grown in spent engine oil contaminated soil

Bangladesh Journal of Scientific and Industrial Research ◽

10.3329/bjsir.v55i4.50965 ◽

2020 ◽

Vol 55 (4) ◽

pp. 261-272

Author(s):

OI Bolanle Ojo ◽

MKC Sridhar

Keyword(s):

Heavy Metals ◽

Contaminated Soil ◽

Root Length ◽

Engine Oil ◽

Lead And Cadmium ◽

Significant Difference ◽

Spent Engine Oil ◽

Number Of Leaves ◽

Collar Diameter ◽

Nauclea Diderrichii

Remediation of environmental pollution has been of great concern in recent years. This study was carried out to investigate the potentials of Nauclea diderrichii seedlings to remediate heavy metals from spent engine oil (SEO) contaminated soil. SEO from diesel and petrol engines in different quantities were applied to each plant using 2 kg soil. Plant height, collar diameter, number of leaves, root length were assessed. Amount of lead and cadmium present in the leaves, stem and root of the seedlings were assessed at the end of the experiment. Result showed that 100 % of the seedlings subjected to various treatments survived for the duration of 5 months. There was no significant difference (α>0.05) in the height of N. diderrichii seedlings. Significant (α=0.05) negative reductions were observed in the seedlings collar diameter, no. of leaves and root length. There was significant difference (α>0.05) in the amount of lead and cadmium extracted by N. diderrichii seedlings. This study has been able to show that N. diderrichii has the ability/potential to phyto-extract heavy metals from soil. Bangladesh J. Sci. Ind. Res.55(4), 261-272, 2020

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