Modeling and Visualizing Heterogeneity of Spatial Patterns of Protein-DNA Interaction from High-Density Chromatin Precipitation Mapping Data

Author(s):  
Juntao Li ◽  
Fajrian Yunus ◽  
Zhu Lei ◽  
Majid Eshaghi ◽  
Jianhua Liu ◽  
...  
2022 ◽  
Vol 14 (2) ◽  
pp. 260
Author(s):  
Eun-Sub Kim ◽  
Seok-Hwan Yun ◽  
Chae-Yeon Park ◽  
Han-Kyul Heo ◽  
Dong-Kun Lee

Extreme heat exposure has severe negative impacts on humans, and the issue is exacerbated by climate change. Estimating spatial heat stress such as mean radiant temperature (MRT) is currently difficult to apply at city scale. This study constructed a method for estimating the MRT of street canyons using Google Street View (GSV) images and investigated its large-scale spatial patterns at street level. We used image segmentation using deep learning to calculate the view factor (VF) and project panorama into fisheye images. We calculated sun paths to estimate MRT using panorama images from Google Street View. This paper shows that regression analysis can be used to validate between estimated short-wave, long-wave radiation and the measurement data at seven field measurements in the clear-sky (0.97 and 0.77, respectively). Additionally, we compared the calculated MRT and land surface temperature (LST) from Landsat 8 on a city scale. As a result of investigating spatial patterns of MRT in Seoul, South Korea, we found that a high MRT of street canyons (>59.4 °C) is mainly distributed in open space areas and compact low-rise density buildings where the sky view factor is 0.6–1.0 and the building view factor (BVF) is 0.35–0.5, or west-east oriented street canyons with an SVF of 0.3–0.55. However, high-density buildings (BVF: 0.4–0.6) or high-density tree areas (Tree View Factor, TVF: 0.6–0.99) showed low MRT (<47.6). The mapped MRT results had a similar spatial distribution to the LST; however, the MRT was lower than the LST in low tree density or low-rise high-density building areas. The method proposed in this study is suitable for a complex urban environment consisting of buildings, trees, and streets. This will help decision makers understand spatial patterns of heat stress at the street level.


2019 ◽  
Vol 29 (01) ◽  
pp. 1850025 ◽  
Author(s):  
Chenyun Dai ◽  
Xiaogang Hu

The human hand is capable of producing versatile yet precise movements largely owing to the complex neuromuscular systems that control our finger movement. This study seeks to quantify the spatial activation patterns of the forearm flexor muscles during individualized finger flexions. High-density (HD) surface electromyogram (sEMG) signals of forearm flexor muscles were obtained, and individual motor units were decomposed from the sEMG. Both macro-level spatial patterns of EMG activity and micro-level motor unit distributions were used to systematically characterize the forearm flexor activation patterns. Different features capturing the spatial patterns were extracted, and the unique patterns of forearm flexor activation were then quantified using pattern recognition approaches. We found that the forearm flexor spatial activation during the ring finger flexion was mostly distinct from other fingers, whereas the activation patterns of the middle finger were least distinguishable. However, all the different activation patterns can still be classified in high accuracy (94–100%) using pattern recognition. Our findings indicate that the partial overlapping of neural activation can limit accurate identification of specific finger movement based on limited recordings and sEMG features, and that HD sEMG recordings capturing detailed spatial activation patterns at both macro- and micro-levels are needed.


2020 ◽  
Vol 176 ◽  
pp. 104939 ◽  
Author(s):  
Lívian Otávio Lecca ◽  
Marcelo Teixeira Paiva ◽  
Camila Stefanie Fonseca de Oliveira ◽  
Maria Helena Franco Morais ◽  
Maria Isabel de Azevedo ◽  
...  

Author(s):  
Tianxiao Jiang ◽  
Tao Jiang ◽  
Taylor Wang ◽  
Shanshan Mei ◽  
Qingzhu Liu ◽  
...  

2019 ◽  
Vol 8 (1) ◽  
pp. 35 ◽  
Author(s):  
YuanJian Tian ◽  
Qi Zhou ◽  
Xiaolin Fu

OpenStreetMap (OSM) is a free map that can be created, edited, and updated by volunteers globally. The quality of OSM datasets is therefore of great concern. Extensive studies have focused on assessing the completeness (a quality measure) of OSM datasets in various countries, but very few have been paid attention to investigating the OSM building dataset in China. This study aims to present an analysis of the evolution, completeness and spatial patterns of OSM building data in China across the years 2012 to 2017. This is done using two quality indicators, OSM building count and OSM building density, although a corresponding reference dataset for the whole country is not freely available. Development of OSM building counts from 2012 to 2017 is analyzed in terms of provincial- and prefecture-level divisions. Factors that may affect the development of OSM building data in China are also analyzed. A 1 × 1 km2 regular grid is overlapped onto urban areas of each prefecture-level division, and the OSM building density of each grid cell is calculated. Spatial distributions of high-density grid cells for prefecture-level divisions are analyzed. Results show that: (1) the OSM building count increases by almost 20 times from 2012 to 2017, and in most cases, economic (gross domestic product) and OSM road length are two factors that may influence the development of OSM building data in China; (2) most grid cells in urban areas do not have any building data, but two typical patterns (dispersion and aggregation) of high-density grid cells are found among prefecture-level divisions.


Author(s):  
D.P. Bazett-Jones ◽  
F.P. Ottensmeyer

Dark field electron microscopy has been used for the study of the structure of individual macromolecules with a resolution to at least the 5Å level. The use of this technique has been extended to the investigation of structure of interacting molecules, particularly the interaction between DNA and fish protamine, a class of basic nuclear proteins of molecular weight 4,000 daltons.Protamine, which is synthesized during spermatogenesis, binds to chromatin, displaces the somatic histones and wraps up the DNA to fit into the small volume of the sperm head. It has been proposed that protamine, existing as an extended polypeptide, winds around the minor groove of the DNA double helix, with protamine's positively-charged arginines lining up with the negatively-charged phosphates of DNA. However, viewing protamine as an extended protein is inconsistent with the results obtained in our laboratory.


Author(s):  
S. McKernan ◽  
C. B. Carter ◽  
D. Bour ◽  
J. R. Shealy

The growth of ternary III-V semiconductors by organo-metallic vapor phase epitaxy (OMVPE) is widely practiced. It has been generally assumed that the resulting structure is the same as that of the corresponding binary semiconductors, but with the two different cation or anion species randomly distributed on their appropriate sublattice sites. Recently several different ternary semiconductors including AlxGa1-xAs, Gaxln-1-xAs and Gaxln1-xP1-6 have been observed in ordered states. A common feature of these ordered compounds is that they contain a relatively high density of defects. This is evident in electron diffraction patterns from these materials where streaks, which are typically parallel to the growth direction, are associated with the extra reflections arising from the ordering. However, where the (Ga,ln)P epilayer is reasonably well ordered the streaking is extremely faint, and the intensity of the ordered spot at 1/2(111) is much greater than that at 1/2(111). In these cases it is possible to image relatively clearly many of the defects found in the ordered structure.


Author(s):  
L. Mulestagno ◽  
J.C. Holzer ◽  
P. Fraundorf

Due to the wealth of information, both analytical and structural that can be obtained from it TEM always has been a favorite tool for the analysis of process-induced defects in semiconductor wafers. The only major disadvantage has always been, that the volume under study in the TEM is relatively small, making it difficult to locate low density defects, and sample preparation is a somewhat lengthy procedure. This problem has been somewhat alleviated by the availability of efficient low angle milling.Using a PIPS® variable angle ion -mill, manufactured by Gatan, we have been consistently obtaining planar specimens with a high quality thin area in excess of 5 × 104 μm2 in about half an hour (milling time), which has made it possible to locate defects at lower densities, or, for defects of relatively high density, obtain information which is statistically more significant (table 1).


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