Voltammetric Approach for In-vivo Detecting Dopamine Level of Rat’s Brain

2011 ◽  
pp. 367-370
Author(s):  
G. C. Chen ◽  
H. Z. Han ◽  
T. C. Tsai ◽  
C. C. Cheng ◽  
J. J. Jason Chen
Keyword(s):  
Dopamine Level

scholarly journals Neuroprotective and Neurorescue Mode of Action of Bacopa monnieri (L.) Wettst in 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine-Induced Parkinson’s Disease: An In Silico and In Vivo Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Babita Singh ◽  
Shivani Pandey ◽  
Mohammad Rumman ◽  
Shashank Kumar ◽  
Prem Prakash Kushwaha ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
In Silico ◽  
Dopaminergic Neurons ◽  
Lipid Peroxide ◽  
Bacopa Monnieri ◽  
Dopamine Level ◽  
Mice Model ◽  
Mptp Administration

Ethnopharmacological Relevance: Parkinson’s disease (PD) is characterized by progressive death of dopaminergic neurons. The presently used medicines only tackle the symptoms of PD, but none makes a dent on the processes that underpin the disease’s development. Herbal medicines have attracted considerable attention in recent years. Bacopa monnieri (L.) Wettst (Brahmi) has been used in Indian Ayurvedic medicine to enhance memory and intelligence. Herein, we assessed the neuroprotective role of Bacopa monnieri (L.) Wettst on Parkinson’s disease.Aim of the Study:Bacopa monnieri (L.) Wettst, a medicinal herb, is widely used as a brain tonic. We investigated the neuroprotective and neurorescue properties of Bacopa monnieri (L.) Wettst extract (BME) in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mice model of PD.Materials and Methods: The mice model of MPTP-induced PD is used in the study. In the neuroprotective (BME + MPTP) and neurorescue (MPTP + BME) experiments, the animals were administered 40 mg/kg body weight BME orally before and after MPTP administration, respectively. Effect of BME treatment was evaluated by accessing neurobehavioral parameters and levels of dopamine, glutathione, lipid peroxide, and nitrites. An in silico study was performed using AutoDock Tools 1.5.6 (ADT).Results: A significant recovery in behavioral parameters, dopamine level, glutathione level, lipid peroxides, and nitrite level was observed in BME-treated mice. Treatment with BME before or after MPTP administration has a protective effect on dopaminergic neurons, as evidenced by a significant decrease in GFAP immunostaining and expression of inducible nitric oxide synthase (iNOS) in the substantia nigra region; however, the degree of improvement was more prominent in mice receiving BME treatment before MPTP administration. Moreover, the in silico study revealed that the constituents of BM, including bacosides, bacopasides, and bacosaponins, can inactivate the enzyme monoamine oxidase B, thus preventing the breakdown of MPTP to MPP+.Conclusion: Our results showed that BME exerts both neuroprotective and neurorescue effects against MPTP-induced degeneration of the nigrostriatal dopaminergic neurons. Moreover, BME may slow down the disease progression and delay the onset of neurodegeneration in PD.

scholarly journals A Modeling Study Suggesting a Possible Pharmacological Target to Mitigate the Effects of Ethanol on Reward-Related Dopaminergic Signaling

2008 ◽  
Vol 99 (5) ◽  
pp. 2703-2707 ◽  
Author(s):  
Michele Migliore ◽  
Claudio Cannia ◽  
Carmen C. Canavier
Keyword(s):  
Dopaminergic Neurons ◽  
Temporal Structure ◽  
Ionic Current ◽  
Brain Regions ◽  
Point Of View ◽  
Dopamine Level ◽  
Addictive Behaviors ◽  
Brain Functions

Midbrain dopaminergic neurons are involved in several critical brain functions controlling goal-directed behaviors, reinforcing/reward processes, and motivation. Their dysfunctions alter dopamine release and contribute to a vast range of neural disorders, from Parkinson's disease to schizophrenia and addictive behaviors. These neurons have thus been a natural target of pharmacological treatments trying to ameliorate the consequences of several neuropathologies. From this point of view, a clear experimental link has been recently established between the increase in the pacemaker frequency of dopaminergic neurons in vitro after acute ethanol application and a particular ionic current ( Ih). The functional consequences in vivo, however, are not clear and they are very difficult to explore experimentally. Here we use a realistic computational model of dopaminergic neurons in vivo to suggest that ethanol, through its effects on Ih, modifies the temporal structure of the spiking activity. The model predicts that the dopamine level may increase much more during bursting than during pacemaking activity, especially in those brain regions with a slow dopamine clearance rate. The results suggest that a selective pharmacological remedy could thus be devised against the rewarding effects of ethanol that are postulated to mediate alcohol abuse and addiction, targeting the specific HCN genes expressed in dopaminergic neurons.

Striatal dopamine level increases in the urinary storage phase in cats: An in vivo microdialysis study

Neuroscience ◽  
2005 ◽  
Vol 135 (1) ◽  
pp. 299-303 ◽  
Author(s):  
T. Yamamoto ◽  
R. Sakakibara ◽  
K. Hashimoto ◽  
K. Nakazawa ◽  
T. Uchiyama ◽  
...  
Keyword(s):  
In Vivo Microdialysis ◽  
Striatal Dopamine ◽  
Dopamine Level ◽  
Microdialysis Study

scholarly journals Effect of Voluntary Wheel Running on Striatal Dopamine Level and Neurocognitive Behaviors after Molar Loss in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-6 ◽  
Author(s):  
Linlin Zhang ◽  
Yi Feng ◽  
Wenliang Ji ◽  
Jianzhang Liu ◽  
Kun Liu
Keyword(s):  
Physical Exercise ◽  
Wheel Running ◽  
Striatal Dopamine ◽  
Control Group ◽  
Dopamine Level ◽  
Sprague Dawley ◽  
Voluntary Wheel Running ◽  
Cognition And Emotion ◽  
Voluntary Wheel

The aim of the present study is to evaluate the effect of voluntary wheel running on striatal dopamine level and behavior of cognition and emotion in molar loss rats. Twenty-four Sprague-Dawley rats were enrolled in this study and randomly divided into following 4 groups: control group (C group), molar loss group (ML group), 1-week physical exercise before molar loss group (1W-ML group), and 4-week physical exercise before molar loss group (4W-ML group). The rats both in 4W-ML and 1W-ML groups were placed in the voluntary running wheel in order to exercise for 4 weeks and 1 week, respectively. Then, the rats in 4W-ML, 1W-M, and ML groups received bilateral molar loss operation. After 10 days, striatal dopamine level was detected by in vivo microdialysis coupled with high-performance liquid chromatography (HPLC) and electrochemical detection. All the rats received behavior test after microdialysis detection. The behavior tests including passive avoidance test were used to assess cognition and elevated plus maze test for emotion. The results indicated that voluntary wheel running promoted striatal dopamine level in rats of molar loss. Behavioral data indicated that voluntary wheel running promoted cognition and emotion recovery after molar loss. Therefore, we concluded physical exercise significantly improved the neurocognitive behaviors and increased the striatal dopamine level after molar loss in rats.

scholarly journals Isolation of Pyran Composition and Anti-Parkinson’s Activity of euphorbia Cyathophora

2019 ◽  
Vol 7 (3) ◽  
pp. 67-74
Author(s):  
S Ruby ◽  
B Jaykar
Keyword(s):  
Phenolic Compounds ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Solvent System ◽  
Phytochemical Analysis ◽  
Muscle Rigidity ◽  
Dopamine Level ◽  
Phenolic Derivative ◽  
Isolated Compound

Aim of the study: The aim of this research was to investigate the phytochemical profile, isolate the phytoconsitutuents, Characterised and evaluation of Anti Parkinson activity of ethanolic extract of Indian Medicinal plant of E.cyathophora Materials and methods: Qualitative phytochemical analysis for their phyto constituents. Ethanol was used to extract the crude bio active compound from whole E. Cyathophora plant. Phenolic derivative were isolated from the ethanolic extract of E. cyathophora by using suitable solvent system and characterised by IR, NMR, MASS spectrophotometric method. The total Neuroprotective activities of E. Cyathophora extract were measured by haloperidol induced experimental animal models. Result: Qualitative phytochemical analysis showed phenolic compounds, Flavonoids, Alkaloids, glycosides, phenolic compounds and Tannins were in high amounts. The isolated compound Phenolic derivative showed various regions from IR, NMR and MASS the functional groups, mass of the molecule and proton were analysed and confirmed. The effects of E. Cyathophora (200, 400mg/kg, p.o) were studied using in vivo behavioural parameters like catalepsy, muscle rigidity and locomotor activity and its effects on neurochemical parameters in rats. The increased haloperidol-induced cataleptic scores were significantly(p<0.01) found to be reduced, with EEEC at dose of 200mg/kg and 400mg/kg(ip). EEEC administration showed significant increase in dopamine level and significant reduced in serotonin and L-glutamate level. Daily administration of EEEC (400mg/kg) significantly improved motor performance. Conclusion: Overall results the study proved that E. Cyathophora possessed potential components involving in anti -Parkinson treatment significantly attenuated the motor defects and also increased the neuro chemical dopamine level. Keywords: E. cyathophora, Anti-Parkinson‟s activity, Ethanol extract, L-Dopa, Carbidopa and Haloperidol.

scholarly journals 2-Pentadecyl-2-oxazoline ameliorates memory impairment and depression-like behaviour in neuropathic mice: possible role of adrenergic alpha2- and H3 histamine autoreceptors

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Serena Boccella ◽  
Francesca Guida ◽  
Monica Iannotta ◽  
Fabio Arturo Iannotti ◽  
Rosmara Infantino ◽  
...  
Keyword(s):  
Neuropathic Pain ◽  
Nerve Injury ◽  
Molecular Mechanisms ◽  
Day Treatment ◽  
Long Term Potentiation ◽  
Dopamine Level ◽  
Term Potentiation ◽  
Report Data ◽  
Target Molecules

AbstractNeuropathic pain (NP) remains an untreatable disease due to the complex pathophysiology that involves the whole pain neuraxis including the forebrain. Sensory dysfunctions such as allodynia and hyperalgesia are only part of the symptoms associated with neuropathic pain that extend to memory and affectivity deficits. The development of multi-target molecules might be a promising therapeutic strategy against the symptoms associated with NP. 2-pentadecyl-2-oxazoline (PEA-OXA) is a plant-derived agent, which has shown effectiveness against chronic pain and associated neuropsychiatric disorders. The molecular mechanisms by which PEA-OXA exerts its effects are, however, only partially known. In the current study, we show that PEA-OXA, besides being an alpha2 adrenergic receptor antagonist, also acts as a modulator at histamine H3 receptors, and report data on its effects on sensory, affective and cognitive symptoms associated with the spared nerve injury (SNI) model of neuropathic pain in mice. Treatment for 14 days with PEA-OXA after the onset of the symptoms associated with neuropathic pain resulted in the following effects: (i) allodynia was decreased; (ii) affective/cognitive impairment associated with SNI (depression, spatial, and working memories) was counteracted; (iii) long-term potentiation in vivo in the lateral entorhinal cortex-dentate gyrus (perforant pathway, LPP) was ameliorated, (iv) hippocampal glutamate, GABA, histamine, norepinephrine and dopamine level alterations after peripheral nerve injury were reversed, (v) expression level of the TH positive neurons in the Locus Coeruleus were normalized. Thus, a 16-day treatment with PEA-OXA alleviates the sensory, emotional, cognitive, electrophysiological and neurochemical alterations associated with SNI-induced neuropathic pain.

Monitoring In Vivo Changes in Tonic Extracellular Dopamine Level by Charge-Balancing Multiple Waveform Fast-Scan Cyclic Voltammetry

2016 ◽  
Vol 88 (22) ◽  
pp. 10962-10970 ◽  
Author(s):  
Yoonbae Oh ◽  
Cheonho Park ◽  
Do Hyoung Kim ◽  
Hojin Shin ◽  
Yu Min Kang ◽  
...  
Keyword(s):  
Cyclic Voltammetry ◽  
Dopamine Level ◽  
Fast Scan Cyclic Voltammetry ◽  
Extracellular Dopamine ◽  
Charge Balancing

scholarly journals CALPAIN ACTIVITY UNDER EXPERIMENTAL INCREASING OF DOPAMINE LEVEL

2019 ◽  
Vol 19 (1S) ◽  
pp. 221-222
Author(s):  
N S Pestereva ◽  
A Z Marshak ◽  
M N Karpenko
Keyword(s):  
Control Group ◽  
Dopamine Level ◽  
Calpain Activity ◽  
In Situ Study ◽  
Calpain 2 ◽  
Casein Zymography ◽  
Experimental Group

The aim of our study was to identify the activity of calpains under conditions of an experimental increase in the level of dopamine. The work was performed at three levels: in vivo, in situ, in vitro. An in situ study was carried on a model of isolated nerve endings - synaptosomes. Using casein zymography in solution with FITC-casein, it was shown that incubation of synaptosomes dopamine leads to calpains secretion into the synaptosomal medium. The dopamine ability to directly activate calpain was demonstrated by casein zymography in a gel. Incubation in an activation buffer containing dopamine instead of the classical activator, calcium chloride, led to the activation of calpain-2. An in vivo experiment was performed on Wistar rats. The experimental group was orally administered the drug L-dopa (100 mg/kg), the control group - saline was injected in the same way.

Fine Structural Changes in the Microvasculature of the Mouse Pinna: Aging and Radiation Injury

1974 ◽  
Vol 32 ◽  
pp. 54-55
Author(s):  
S. Phyllis Steamer ◽  
Rosemarie L. Devine
Keyword(s):  
Structural Changes ◽  
Radiation Treatment ◽  
Arterial Stenosis ◽  
Ultrastructural Changes ◽  
Vascular Effects ◽  
Microvascular Changes ◽  
Surrounding Connective Tissue ◽  
Fission Neutrons ◽  
Total Body

The importance of radiation damage to the skin and its vasculature was recognized by the early radiologists. In more recent studies, vascular effects were shown to involve the endothelium as well as the surrounding connective tissue. Microvascular changes in the mouse pinna were studied in vivo and recorded photographically over a period of 12-18 months. Radiation treatment at 110 days of age was total body exposure to either 240 rad fission neutrons or 855 rad 60Co gamma rays. After in vivo observations in control and irradiated mice, animals were sacrificed for examination of changes in vascular fine structure. Vessels were selected from regions of specific interest that had been identified on photomicrographs. Prominent ultrastructural changes can be attributed to aging as well as to radiation treatment. Of principal concern were determinations of ultrastructural changes associated with venous dilatations, segmental arterial stenosis and tortuosities of both veins and arteries, effects that had been identified on the basis of light microscopic observations. Tortuosities and irregularly dilated vein segments were related to both aging and radiation changes but arterial stenosis was observed only in irradiated animals.

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