Catharanthus roseus (Periwinkle): In Vitro Culture, and High-Level Production of Arbutin by Biotransformation

1998 ◽  
pp. 67-80
Author(s):  
M. Yokoyama ◽  
S. Inomata
Keyword(s):  
In Vitro Culture ◽  
Catharanthus Roseus ◽  
High Level ◽  
Level Production

scholarly journals EFEKTIVITAS MERKURI KLORIDA (HgCl2) PADA STERILISASI TUNAS SAMPING JATI (Tectona grandis) IN VITRO

2017 ◽  
Vol 4 (2) ◽  
pp. 25
Author(s):  
Yusuf Sigit Ahmad Fauzan ◽  
. Supriyanto ◽  
Teuku Tajuddin
Keyword(s):  
In Vitro Culture ◽  
Tectona Grandis ◽  
In Vitro Cultures ◽  
Mercury Chloride ◽  
Main Obstacle ◽  
Sterilization Process ◽  
High Level ◽  
Growth Of Fungi ◽  
Tectona Grandis L.F

Effectiveness of Mercury Chloride (HgCl2) in Sterilization of Teak (Tectona grandis L.f.) In VitroThe main obstacle in obtaining sterile materials in in vitro cultures derived from meristems is high level of surface contamination caused by fungi and bacteria, which often results in explant death. The objective of this study was to obtain an appropriate mercury chloride (HgCl2) concentration for the sterilization of Tectona grandis nodes in in vitro culture. One cm long-sized nodes with 0.2 mm diameter were immersed in HgCl2at concentrations of 0, 100, 200 and 300 mg/L for 3 minutes. The results showed that the higher concentration of HgCl2was able to suppress the growth of fungi and bacteria and increased the percentage of aseptic explants. The best HgCl2concentration was 300 mg/L since it suppressed the growth of fungi and bacteria up to 100% and 75%, respectively, and produced the highest aseptic explants of 85% at 9 days after treatment. The small sized explants supported the sterilization process and reduced browning levels.Keywords: Browning, HgCl2, in vitro, sterilization, T. grandisABSTRAKKendala utama dalam mendapatkan material steril pada kultur in vitro yang berasal dari meristem adalah tingginya tingkat kontaminasi permukaan yang disebabkan oleh jamur dan bakteri, dan sering menyebabkan kematian eksplan. Tujuan penelitian ini adalah untuk memperoleh konsentrasi merkuri klorida (HgCl2) yang tepat untuk sterilisasi eksplan tunas samping tanaman jati (Tectona grandis) pada kultur in vitro. Tunas samping berukuran 1 cm dan diameter 0,2 mm direndam dalam HgCl2 pada konsentrasi 0, 100, 200 dan 300 mg/L selama 3 menit. Hasil penelitian menunjukkan bahwa penambahan konsentrasi HgCl2 yang semakin tinggi mampu menekan pertumbuhan jamur dan bakteri pada eksplan serta meningkatkan persentase eksplan aseptik. HgCl2 dengan konsentrasi 300 mg/L merupakan konsentrasi terbaik karena dapat menekan pertumbuhan jamur hingga 100% dan bakteri mencapai 75%, serta menghasilkan tingkat eksplan aseptik dan hidup tertinggi yaitu sebesar 85% pada 9 hari setelah perlakuan. Ukuran eksplan yang kecil mendukung proses sterilisasi dan mengurangi tingkat browning. Kata kunci: HgCl2,in vitro, pencoklatan jaringan, sterilisasi, T. grandis, Received: 02 November 2017                 Accepted: 14 December 2017                Published: 29 December 2017

scholarly journals High-Level Production of High-Purity Human and Murine Recombinant Prion Proteins Functionally Compatible to In Vitro Seeding Assay

2018 ◽  
Vol 28 (10) ◽  
pp. 1749-1759
Author(s):  
Hae-Gwang Hwang ◽  
Dae-Hwan Kim ◽  
Jeongmin Lee ◽  
Youngwon Mo ◽  
Se-Hoon Lee ◽  
...  
Keyword(s):  
High Purity ◽  
Prion Proteins ◽  
High Level ◽  
Level Production

scholarly journals The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture

2021 ◽  
Vol 28 (1) ◽  
pp. 39
Author(s):  
Mouleidi Dwi Putri ◽  
Windri Handayani ◽  
Astari Dwiranti ◽  
Andi Salamah ◽  
Niarsi Merry Hemelda ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Common Type ◽  
Internal Contamination ◽  
Sterilization Method ◽  
Combination Treatments ◽  
Sterilization Process ◽  
Bacteria And Fungi ◽  
High Level

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.

scholarly journals The induction and development of somatic embryos from the in vitro culture of Catharanthus roseus (L.) G. Don

2021 ◽  
Vol 35 (1) ◽  
Author(s):  
H. Farhadi ◽  
M.B. Hassanpouraghdam ◽  
Mohammad Ali Aazami
Keyword(s):  
Somatic Embryogenesis ◽  
In Vitro Culture ◽  
Catharanthus Roseus ◽  
Somatic Embryos ◽  
Leaf Explants ◽  
Fresh Weight ◽  
Efficient Treatment ◽  
Hypocotyl Explants ◽  
Important Medicinal Plant

Catharanthus roseus is containing anticancer alkaloids vincristine and vinblastine as is an important medicinal plant. Several studies have conducted on in-vitro culture of this plant. To optimize the somatic embryogenesis, a factorial based on CRD experiment with 10 replications was conducted. Root, hypocotyl and leaf explants grown in-vitro were transferred and cultured on MS media containing different combinations of 2,4-D, NAA and 2,4-D×BAP. The results revealed that in callogenesis, the interaction effects of root and hypocotyl explants×2,4-D and NAA as well as hypocotyl×(1 mg l-1 NAA+1 mg l-1 BAP) was superior than other treatments (p≤0.01). For calli fresh weight, hypocotyl×NAA and hypocotyl×(1 mg l-1 NAA+1 mg l-1 BAP) was the treatment of choice (p≤0.01). The calli produced were sub-cultured to attain the pre-embryos and somatic embryos. For the number of pre-embryos and somatic embryos; the interaction of hypocotyl×2,4-D was the best and most efficient treatment. Seemingly, the production of somatic embryos is accessible in this plant by the logical management of growth regulator combinations. Furthermore, the production and genetic engineering of the somatic embryos could be a promising trend in the subsequent production of high-valued metabolites from this plant.

scholarly journals Increased responsiveness of B. cells in the murine MRL/lpr model of lupus nephritis to interleukin-1 beta.

1995 ◽  
Vol 5 (7) ◽  
pp. 1530-1534
Author(s):  
T V Lebedeva ◽  
A K Singh
Keyword(s):  
B Cells ◽  
Lupus Nephritis ◽  
B Cell ◽  
Interleukin 1 ◽  
Autoantibody Production ◽  
Dna Antibodies ◽  
High Concentrations ◽  
High Level ◽  
Level Production

The high-level production of immunoglobulin G (IgG), anti-DNA antibodies, and id-H130-expressing antibodies has been correlated with both the presence and the severity of nephritis in the murine MRL/lpr lupus model. Although evidence suggests that interleukin-1 (IL-1) could be an important factor in the immunopathogenesis of murine lupus nephritis, its influence on B cell hyperactivity is poorly understood. The in vitro responsiveness of B cells derived from lupus-prone old and young MRL/lpr and healthy C3H/HeJ mice to exogenous IL-1 beta was examined. B cells derived from MRL/lpr mice, and particularly old MRL/lpr mice, were hyperresponsive to exogenous IL-1 beta, demonstrating a marked increase in IgG production with 50 pg/mL concentrations of IL-1 beta as compared with control medium. Whereas MRL/lpr B cells demonstrated remarkable unresponsiveness to high concentrations of IL-1 beta. By contrast, B cells derived from C3H/HeJ mice and cultured with IL-1 beta showed virtually no alteration in IgG production. In addition, B cells derived from old MRL/lpr mice and cultured with IL-1 beta showed a significant increase in the production of anti-DNA and id-H130-expressing antibodies. Collectively, these observations demonstrate increased B cell responsiveness to exogenous IL-1 beta and suggest that heightened IL-1 bioactivity in the murine MRL/lpr lupus model may influence high-level IgG and autoantibody production.

scholarly journals The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

2021 ◽  
Vol 28 (2) ◽  
pp. 110
Author(s):  
Anna Widyastuti ◽  
Afiatry Putrika ◽  
Astari Dwiranti ◽  
Andi Salamah ◽  
Niarsi Merry Hemelda ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Shoot Formation ◽  
Common Type ◽  
In Vitro Cultures ◽  
Sterilization Process ◽  
Bacteria And Fungi ◽  
High Level ◽  
Viable Culture

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.

Isohexenylnaphthazarins from Lithospermum canescens (Michx.) Lehm. and Arnebia euchroma (Royle) Jonst. in vitro culture

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Graikou ◽  
H Damianakos ◽  
K Syklowska-Baranek ◽  
A Pietrosiuk ◽  
M Jeziorek ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Arnebia Euchroma ◽  
Lithospermum Canescens
Sign in / Sign up

Export Citation Format

Share Document