scholarly journals EFEKTIVITAS MERKURI KLORIDA (HgCl2) PADA STERILISASI TUNAS SAMPING JATI (Tectona grandis) IN VITRO

2017 ◽  
Vol 4 (2) ◽  
pp. 25
Author(s):  
Yusuf Sigit Ahmad Fauzan ◽  
. Supriyanto ◽  
Teuku Tajuddin
Keyword(s):  
In Vitro Culture ◽  
Tectona Grandis ◽  
In Vitro Cultures ◽  
Mercury Chloride ◽  
Main Obstacle ◽  
Sterilization Process ◽  
High Level ◽  
Growth Of Fungi ◽  
Tectona Grandis L.F

Effectiveness of Mercury Chloride (HgCl2) in Sterilization of Teak (Tectona grandis L.f.) In VitroThe main obstacle in obtaining sterile materials in in vitro cultures derived from meristems is high level of surface contamination caused by fungi and bacteria, which often results in explant death. The objective of this study was to obtain an appropriate mercury chloride (HgCl2) concentration for the sterilization of Tectona grandis nodes in in vitro culture. One cm long-sized nodes with 0.2 mm diameter were immersed in HgCl2at concentrations of 0, 100, 200 and 300 mg/L for 3 minutes. The results showed that the higher concentration of HgCl2was able to suppress the growth of fungi and bacteria and increased the percentage of aseptic explants. The best HgCl2concentration was 300 mg/L since it suppressed the growth of fungi and bacteria up to 100% and 75%, respectively, and produced the highest aseptic explants of 85% at 9 days after treatment. The small sized explants supported the sterilization process and reduced browning levels.Keywords: Browning, HgCl2, in vitro, sterilization, T. grandisABSTRAKKendala utama dalam mendapatkan material steril pada kultur in vitro yang berasal dari meristem adalah tingginya tingkat kontaminasi permukaan yang disebabkan oleh jamur dan bakteri, dan sering menyebabkan kematian eksplan. Tujuan penelitian ini adalah untuk memperoleh konsentrasi merkuri klorida (HgCl2) yang tepat untuk sterilisasi eksplan tunas samping tanaman jati (Tectona grandis) pada kultur in vitro. Tunas samping berukuran 1 cm dan diameter 0,2 mm direndam dalam HgCl2 pada konsentrasi 0, 100, 200 dan 300 mg/L selama 3 menit. Hasil penelitian menunjukkan bahwa penambahan konsentrasi HgCl2 yang semakin tinggi mampu menekan pertumbuhan jamur dan bakteri pada eksplan serta meningkatkan persentase eksplan aseptik. HgCl2 dengan konsentrasi 300 mg/L merupakan konsentrasi terbaik karena dapat menekan pertumbuhan jamur hingga 100% dan bakteri mencapai 75%, serta menghasilkan tingkat eksplan aseptik dan hidup tertinggi yaitu sebesar 85% pada 9 hari setelah perlakuan. Ukuran eksplan yang kecil mendukung proses sterilisasi dan mengurangi tingkat browning. Kata kunci: HgCl2,in vitro, pencoklatan jaringan, sterilisasi, T. grandis, Received: 02 November 2017                 Accepted: 14 December 2017                Published: 29 December 2017

scholarly journals The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp.

2021 ◽  
Vol 28 (2) ◽  
pp. 110
Author(s):  
Anna Widyastuti ◽  
Afiatry Putrika ◽  
Astari Dwiranti ◽  
Andi Salamah ◽  
Niarsi Merry Hemelda ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Shoot Formation ◽  
Common Type ◽  
In Vitro Cultures ◽  
Sterilization Process ◽  
Bacteria And Fungi ◽  
High Level ◽  
Viable Culture

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.

scholarly journals The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture

2021 ◽  
Vol 28 (1) ◽  
pp. 39
Author(s):  
Mouleidi Dwi Putri ◽  
Windri Handayani ◽  
Astari Dwiranti ◽  
Andi Salamah ◽  
Niarsi Merry Hemelda ◽  
...  
Keyword(s):  
In Vitro Culture ◽  
Exposure Time ◽  
Common Type ◽  
Internal Contamination ◽  
Sterilization Method ◽  
Combination Treatments ◽  
Sterilization Process ◽  
Bacteria And Fungi ◽  
High Level

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.

scholarly journals MTMS-Based Aerogel Constructs for Immobilization of Plant Hairy Roots: Effects on Proliferation of Rindera graeca Biomass and Extracellular Secretion of Naphthoquinones

2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Bartosz Nowak ◽  
Mateusz Kawka ◽  
Kamil Wierzchowski ◽  
Katarzyna Sykłowska-Baranek ◽  
Maciej Pilarek
Keyword(s):  
Polyurethane Foam ◽  
Bioactive Compounds ◽  
Hairy Roots ◽  
Reference System ◽  
Culture System ◽  
In Vitro Cultures ◽  
Fresh Mass ◽  
Extracellular Secretion ◽  
High Level

Unique biosynthetic abilities revealed by plants determine in vitro cultures of hairy roots as a suitable source of pharmaceutically relevant bioactive compounds. The basic aim of the study was to examine the applicability of aerogel composed of methyltrimethoxysilane (MTMS) for immobilization of Rindera graeca hairy roots by identifying quantitative effects of biomass proliferation and naphthoquinones extracellular secretion in the aerogel-supported culture system. R. graeca hairy roots were simultaneously cultured for 28-days, as (i) nonimmobilized biomass (reference system), (ii) biomass immobilized on macroporous polyurethane foam (PUF), (iii) biomass with disintegrated MTMS aerogel, (iv) biomass immobilized on polypropylene (PP) fibers (as control), and (v) biomass immobilized on monolithic PP-reinforced MTMS aerogel. MTMS aerogel exhibited high level of biocompatibility toward R. graeca hairy roots which grew into the structure of monolithic aerogel-based constructs. Monolithic MTMS-based constructs significantly promoted the proliferation of hairy roots, resulting in 55% higher fresh mass than the reference system. The highest level of naphthoquinones productivity, i.e., 653 µg gDW−1, was noted for PUF-supported culture system.

scholarly journals Optimasi sterilisasi permukaan eksplan stek mikro tanaman karet Optimization of surface sterilization on rubber microcutting explan

2016 ◽  
Vol 81 (1) ◽  
Author(s):  
Irfan MARTIANSYAH ◽  
Deden Dewantara ERIS ◽  
. NURHAIMI-HARIS ◽  
Darmono TANIWIRYONO
Keyword(s):  
Primary Culture ◽  
Second Step ◽  
Contamination Level ◽  
Surface Sterilization ◽  
Major Constraint ◽  
Culture Stage ◽  
Sterilization Process ◽  
High Level

AbstractAn increasing number of explants is necessary toobtain plantlets in large quantities, for mass propagationof rubber plants. However, high level of contamination atthe primary culture stage is still a major constraint in invitro microcutting of rubber. The aim of this study was tooptimize surface sterilization procedures to reduce micro-bial contamination at the primary culture. Sterilizationexperiment was conducted in two step., The first step wasto determine the effect of washing the explants withrunning water prior to sterilization and then using Deso-germe, ethanol or H 2 O 2 , while the second step was toidentify the suitable sterilization process on reducing thelevel of contamination. The results showed that the surfacesterilization with only one type of sterilization agent couldnot reduce contamination level caused either by bacteriaor fungi, while sterilization with three types of sterilizingagents increased the number of dead explants. The besttreatment for surface sterilization was the directsterilization of explants using 70% ethanol for one minuteand 17.6% H 2 O 2 for 20 minutes without washing with tapwater (A-CD treatment). The percentage of viable andaseptic explantsof this treatment was 76.7%, which wassignificantly higher than those of other treatments. Thistreatment reduced contamination level to 21.7%.AbstrakPeningkatan jumlah eksplan sangat diperlukan untukmemperoleh planlet dalam jumlah besar pada perbanyakanmassal tanaman karet secara in vitro. Namun, tingginyatingkat kontaminasi pada tahap kultur primer masih me-rupakan kendala utama dalam kultur stek mikro tanamankaret. Tujuan penelitian adalah mengoptimasi prosedursterilisasi permukaan eksplan untuk mengurangi jumlaheksplan yang terkontaminasi mikroba pada tahap kulturprimer. Percobaan sterilisasi dilaksanakan dalam duatahap, tahap pertama untuk mengetahui pengaruh pen-cucian eksplan dengan air mengalir pada awal sterilisasiserta penggunaan Desogerme, etanol dan H 2 O 2 , sedang-kan tahap kedua untuk mendapatkan proses sterilisasi yangpaling sesuai dalam menurunkan tingkat kontaminasi.Hasil penelitian menunjukkan bahwa perlakuan sterilisasipermukaan yang menggunakan satu jenis bahan sterilantidak dapat mengurangi kontaminasi, baik oleh bakterimaupun cendawan. Perlakuan sterilisasi eksplan dengantiga jenis bahan sterilan meningkatkan kematian eksplan.Perlakuan sterilisasi permukaan terbaik adalah sterilisasilangsung eksplan menggunakan etanol 70% selama satu  menit dan H 2 O 2 17,6% selama 20 menit, tanpa pencuciandengan air mengalir (perlakuan A-CD). Persentase eksplansteril yang hidup sebesar 76,7%, berbeda nyata dibanding-kan dengan perlakuan lainnya. Perlakuan tersebut dapatmengurangi kontaminasi menjadi sebesar 21,7%.

scholarly journals Results with the establishment of in vitro culture of Leucojum aestivum

2007 ◽  
Vol 13 (2) ◽  
Author(s):  
E. Kohut ◽  
M. Ördögh ◽  
E. Jámbor-Benczúr ◽  
Á. Máthé
Keyword(s):  
Acetic Acid ◽  
Medicinal Plant ◽  
In Vitro Culture ◽  
In Vitro Cultures ◽  
Naphthalene Acetic Acid ◽  
Benzyl Adenine ◽  
Leucojum Aestivum ◽  
Leaves And Roots ◽  
Bulbous Plant

Leucojum aestivum is a native, protected ornamental and medicinal plant in Hungary and in Ukraine too. The aim of our work was to establish in vitro cultures of this bulbous plant. Prior to surface sterilisation the old leaves and roots were dissected from the bulbs and they were stored in a refrigerator (2-3°C) for different periods (1 week for the first starting experiment and 5 weeks for the second one). After sterilisation, bulbs, bulb scales and leaves of the bulbs were placed on Murashige and Skoog's (1962) medium with 1 mg/1 benzyl-adenine (BA) and 0,1 mg/1 naphthalene acetic acid (NAA). At the first starting experiment 81,3%, and at the second one 92,3% of the explants turned to be sterile. Bulblets and roots were developed on the explants in the case of using bulb plates together with bulb scales and leaves as inoculua. The best result was achieved after 5 weeks chilling and it was possible to gain little bulbs from the bulb leaves too.

scholarly journals EFEITO DE DIFERENTES CONCENTRAÇÕES DE NITROGÊNIO E DE SACAROSE NA PROPAGAÇÃO in vitro DE TECA (Tectona grandis L.f.)

2013 ◽  
Vol 86 (3) ◽  
pp. 222
Author(s):  
Dayane Ávila Fernandes ◽  
Luana Pâmella de Almeida Azevedo ◽  
Reginaldo Brito da Costa
Keyword(s):  
Tectona Grandis ◽  
Tectona Grandis L.F

A teca (Tectona grandis L. f.) é uma espécie arbórea considerada uma das madeiras mais valiosas do mundo. A micropropagação desse germoplasma surgiu como alternativa para a obtenção de mudas de alta qualidade, com características desejáveis e em escala comercial. Adicionalmente, pesquisas têm sido realizadas visando reduzir os custos da técnica. O presente estudo objetivou avaliar o desenvolvimento in vitro de Tectona grandis L.f., sob a influência de diferentes concentrações de nitrato de potássio (KNO3) e de sacarose no meio de cultura. O delineamento experimental foi o inteiramente casualizado em esquema fatorial 5x4, sendo cinco concentrações de KNO3 (0, 50, 75, 100 e 125%) e quatro concentrações de sacarose (12, 18, 24 e 30 g.L-1), totalizando vinte tratamentos com dez repetições cada. Os explantes foram cultivados em meio MS, com pH ajustado para 5,8. O meio de cultura semi-sólido foi acrescido de 7 g.L-1 de ágar, e posteriormente esterilizado a 120 ºC e 1,5 atm por 25 minutos. Após a inoculação, os explantes foram mantidos por 45 dias em sala de crescimento com temperatura de 30 +2 ºC e fotoperíodo de 16 h. Avaliaram-se as seguintes variáveis: matéria seca, comprimento da plântula e número de entrenós dos brotos. Para as três variáveis testadas, apenas a ausência de KNO3 se mostrou estatisticamente diferente das outras concentrações, mostrando que podem ser utilizadas menores doses desse macronutriente. Os resultados demonstraram a possibilidade de reduzir a concentração de sacarose, sem comprometer o desenvolvimento da teca. Os melhores resultados foram obtidos nas concentrações de 12, 18 e 30 g.L-1.

scholarly journals TIPOS DE VEDAÇÃO E CONCENTRAÇÕES DE SACAROSE NO CULTIVO in vitro DE Tectona grandis L.f.

2013 ◽  
Vol 88 (3) ◽  
pp. 218
Author(s):  
Dayane Ávila Fernandes ◽  
Patrícia Helena de Azevedo ◽  
Reginaldo Brito da Costa ◽  
Gilvano Ebling Brondani
Keyword(s):  
Tectona Grandis ◽  
Tectona Grandis L.F

O presente estudo objetivou testar concentrações de sacarose e tipos de vedação dos frascos no cultivo in vitro de teca (Tectona grandis L.f.). O delineamento experimental foi inteiramente casualizado em esquema fatorial (2x6), sendo dois tipos de vedação (tampa com filtro e tampa sem filtro) e seis concentrações de sacarose (0, 6, 12, 18, 24 e 30 mg.L-1), totalizando doze tratamentos com dez repetições. Os explantes foram cultivados em meio de cultura MS e mantidos por 35 dias em sala de crescimento com temperatura de 30 ± 2 ºC e fotoperíodo de 16 h. As seguintes variáveis foram determinadas: comprimento de broto (cm), número de entrenós e matéria seca dos brotos (mg). Os melhores resultados foram obtidos com o uso da tampa com filtro. Para cada variável, a melhor concentração de sacarose foi diferente. Na concentração de 17 mg.L-1 de sacarose, os brotos apresentaram 4,1 cm de comprimento. Na concentração de 18 mg.L-1, o número de entrenós, foi de 4,6 e para a matéria seca, a melhor concentração foi 30 mg.L-1, com média de 0,0162 mg. Os melhores resultados foram obtidos com 18 mg.L-1 de sacarose e a tampa plástica com filtro.

scholarly journals Effect of various plant growth regulators on in vitro seed germination and shoot organogenesis in Tectona grandis L.f.

2018 ◽  
Vol 5 (2) ◽  
pp. 152-159
Author(s):  
Jay Prakash Mishra ◽  
◽  
Deepti Bhadrawale ◽  
Upasana Yadav ◽  
Naseer Mohammad ◽  
...  
Keyword(s):  
Seed Germination ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Organogenesis ◽  
Tectona Grandis ◽  
Tectona Grandis L.F

scholarly journals SACAROSE E ÁCIDO INDOLBUTÍRICO NO ENRAIZAMENTO DE Tectona grandis L.f.

2015 ◽  
Vol 90 (1) ◽  
pp. 87
Author(s):  
Dayane Ávila Fernandes ◽  
Diego Tyszka Martinez ◽  
Reginaldo Brito da Costa
Keyword(s):  
Tectona Grandis ◽  
Tectona Grandis L.F

O presente estudo objetivou testar a sacarose e o ácido indolbutírico (AIB) no enraizamento de microestacas de teca (Tectona grandis L.f.). As bases das microestacas foram imersas nas soluções e, em seguida, plantadas nos tubetes com substrato à base de casca de pinus bioestabilizada. O experimento foi dividido em três fases: casa de vegetação, casa de sombra e pleno sol. Foram avaliados: comprimento da parte aérea (cm), diâmetro do colo (cm) e peso da matéria fresca (mg). Os resultados demonstraram haver interação entre cultivo e sacarose apenas na casa de vegetação (primeira avaliação) para as três variáveis analisadas. A concentração de AIB não apresentou diferença entre as avaliações e o uso de sacarose mostrou-se necessário principalmente quando houve sua redução no cultivo in vitro.

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