Cell Surface Labeling for the Scanning Electron Microscope

1978 ◽  
pp. 65-88 ◽  
Author(s):  
S. S. Brown ◽  
J.-P. Revel
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Scanning Electron

Cell Border Demarcation in the Scanning Electron Microscope by Silver Stains

1974 ◽  
Vol 16 (1) ◽  
pp. 221-239
Author(s):  
W. C. DE BRUIJN ◽  
W. VAN MOURIK ◽  
I. J. BOSVELD
Keyword(s):  
Endothelial Cells ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Silver Nitrate ◽  
Aortic Tissue ◽  
Cell Border ◽  
Thorium Dioxide ◽  
Dextran Solution ◽  
Scanning Electron

Silver nitrate and silver proteinate were both successfully used as electron stains for the demarcation of the aortic endothelial cell borders in the scanning electron microscope. By energy-dispersive X-ray analysis it was demonstrated that silver was present in the demarcation lines around the endothelial cells. The presence of silver closely coincided with the places where secondary electrons were produced. Judged by the quality of the aortic tissue in the transmission electron microscope, the method using silver proteinate was preferable to those using silver nitrate. The limited resolution of the method is demonstrated. The role of the dextran solution in such procedures was investigated, and it was shown that the presence of such a dextran solution, or its particle size or charge, does not contribute to the formation of these silver lines around the endothelial cells. In ultrathin sections of such material the cell surface-contrasting capacity of colloidal thorium dioxide solutions was compared to the cell border-contrasting capacity of silver proteinate. It was found that prior aldehyde fixation abolished the cell border demarcation by silver proteinate, but only reduced the cell surface demarcation by thorium dioxide particles.

scholarly journals Simultaneous visualization of myeloperoxidase reactivity and immunogold labeling by backscattered electron imaging with the scanning electron microscope.

1987 ◽  
Vol 35 (2) ◽  
pp. 267-269 ◽  
Author(s):  
D Soligo ◽  
E P de harven
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Cell Types ◽  
Myeloperoxidase Activity ◽  
Backscattered Electron Imaging ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Simultaneous Visualization ◽  
Scanning Electron

We describe a method to label circulating human granulocytes with an immunogold marker and then incubate them for demonstration of myeloperoxidase activity. The cells were observed in the backscattered electron imaging (BEI) mode of the scanning electron microscope. This permits simultaneous visualization of cell surface morphology, the immunological surface marker, and the cytochemical reactivity of each individual cell. Enhanced identification of the various cell types and more precise characterization of cell surface features in the different steps of leukocyte differentiation are expected to result from application of this technique.

scholarly journals Fruit micromorphology of Siberian Apiaceae and its value for taxonomy of the family

Turczaninowia ◽  
2021 ◽  
Vol 24 (2) ◽  
pp. 120-143
Author(s):  
Tatyana A. Ostroumova
Keyword(s):  
Cell Wall ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Species Identification ◽  
Relative Position ◽  
Wide Distribution ◽  
Cultivated Plants ◽  
The Family ◽  
Scanning Electron

Using a scanning electron microscope, the fruit micromorphology of the family Apiaceae of Siberia was studied. In total, the study covered 97 species of wild, adventive, and the most important cultivated plants. Within one fruit, micromorphology is heterogeneous; the most informative is the surface of the grooves and the rib bases. Exocarp cells are described (relative position, shape, size, nature of the boundaries between cells, outer walls, and fine relief of the cell wall). Characters most consistent within the species, for example, outgrowths on cell surface and a thin relief of cell wall, are noted. The characteristics of the ridges of the ribs, stomata (their presence, position on the surface of the fruit, and cuticle on the cells adjacent to the stomata), and epicuticular wax are also given. 25 species have hairs, scales, spines or warts on the surface; the shape of these structures and their fine relief are described. The importance of micromorphology for species identification and taxonomy is shown. The wide distribution of parallel variability in the family is noted.

Accumulation of the material containing the toxin cerato-ulmin on the hyphal surface of Ceratocystis ulmi

1980 ◽  
Vol 58 (6) ◽  
pp. 663-668 ◽  
Author(s):  
Shozo Takai ◽  
Yasuyuka Hiratsuka
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Water Extract ◽  
Water Extractable ◽  
Scanning Electron

Recovery of cerato-ulmin (CU) in the water extract from intact hyphae of Ceratocystis ulmi proved that it is present on the hyphal surface. Degrees of the accumulation of unidentified material on the cell surface of hyphae, together with CU recovery, varied from high, among isolates classified as distinct in CU production, to unmeasurable, among isolates classified as negligible in CU production. There was a correlation between the accumulation of unidentified material on the hyphal surface as observed under the scanning electron microscope (SEM) and recovery of CU from hyphal colonies. Most of the accumulated material was water-extractable, as is CU, and it is therefore concluded that the material accumulated on the hyphae of isolates classified as distinct in CU production does, indeed, contain CU.

Mechanisms of nutritive endocytosis. I. Phagocytic versatility and cellular recognition in Chlorohydra digestive cells, a scanning electron microscope study

1981 ◽  
Vol 49 (1) ◽  
pp. 311-339
Author(s):  
P.L. McNeil
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Surface ◽  
Surface Coating ◽  
Surface Membrane ◽  
Digestive Cell ◽  
Scanning Electron Microscope Study ◽  
Digestive Cells ◽  
Symbiotic Algae ◽  
Scanning Electron

The quantity of surface membrane internalized during phagocytosis by Chlorohydra digestive cells was estimated for a range of particle types. Challenge with 2 of these particles, freshly isolated symbiotic algae (FIS) and latex spheres (LS), resulted in a greater (2.5 X) quantity of surface membrane interiorized than with heat-treated symbiotic algae (HTS) and free living algae (FA), Chlorella vulgaris. This discriminatory process was investigated further by a scanning electron microscope (SEM) and transmission electron microscope (TEM) comparison of the surface events associated with phagocytosis of each of these 4 particles. Those particles that were avidly phagocytized, FIS and LS, were both enveloped by a tightly fitting extension of digestive-cell surface, and obtained a prominent surface coating after their injection into the gut of Chlorohydra. Phagocytic challenge with FIS resulted, furthermore, in the rapid formation of a dense microvillar cover on digestive-cell surfaces. HTS and FA, on the other hand, were enveloped by a less closely fitting extension of digestive-cell surface, did not obtain a prominent surface coating, and did not induce the formation of microvilli. In addition, SEM revealed that at least 3 morphologically distinct phagocytic modes were utilized by the versatile nutritive phagocyte of Chlorohydra: (I) envelopment by the progressive movement of numerous, overlapping tubular protrusions (microvilli) over the particle (FIS) surface, forming first a network of tubular interlocking members, and finally a continuous but rough enclosing surface; (2) envelopment by a single, smooth-surfaced, funnel-like extension of digestive-cell surface (FIS, LS, HTS, FA); and (3) envelopment by multiple, broad folds, often of unequal size, and with overlapping margins (Artemia particles).

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