Kinetics of CO2 Uptake by Red Cells

1980 ◽  
pp. 299-305 ◽  
Author(s):  
R. A. B. Holland
Keyword(s):  
Red Cells ◽  
Co2 Uptake ◽  
Kinetics Of

Comparative Clearance Kinetics of Beat Damaged (Hdrc) and Antibody Coated Red Cells (Igrc)

1982 ◽  
Vol 62 (2) ◽  
pp. 7P-7P ◽  
Author(s):  
A.M. Peters ◽  
M. J. Walport ◽  
K. B. Elkon ◽  
P. P. Ferjencik ◽  
G. R. V. Hughes ◽  
...  
Keyword(s):  
Red Cells ◽  
Clearance Kinetics ◽  
Kinetics Of

Acetylcholinesterase in Human Erythroid Cells

1973 ◽  
Vol 12 (3) ◽  
pp. 911-923
Author(s):  
R. J. SKAER
Keyword(s):  
Endoplasmic Reticulum ◽  
Nervous System ◽  
Golgi Apparatus ◽  
Red Cells ◽  
The Other ◽  
Red Cell ◽  
Erythroid Cells ◽  
Cell Replacement ◽  
Kinetics Of ◽  
Human Red Cells

Acetylcholinesterase is present in human red cells but cannot be demonstrated by the copper thiocholine test. The enzyme is revealed, however, in the perinuclear cisterna, endoplasmic reticulum and Golgi apparatus of red cell precursors. It is suggested that 2 forms of the enzyme are present, one of which can be demonstrated by the copper thiocholine test, the other cannot; one form may be the precursor of the other. These observations may cast light on the kinetics of red cell replacement and on the interpretation of the results from the copper thiocholine test on other tissues such as the nervous system.

Photographic, stereological and statistical methods in evaluation of aggregation of red cells in disease: Part I: Kinetics of aggregation

Biorheology ◽  
1982 ◽  
Vol 19 (4) ◽  
pp. 567-577 ◽  
Author(s):  
L. Dintenfass ◽  
H. Jedrzejczyk ◽  
A. Willard
Keyword(s):  
Statistical Methods ◽  
Red Cells ◽  
Kinetics Of

scholarly journals Kinetics of Cl-dependent K fluxes in hyposmotically swollen low K sheep erythrocytes.

1991 ◽  
Vol 97 (2) ◽  
pp. 173-193 ◽  
Author(s):  
E Delpire ◽  
P K Lauf
Keyword(s):  
Red Blood Cells ◽  
Kinetic Study ◽  
Kinetic Parameters ◽  
Blood Cells ◽  
Red Cells ◽  
Maximal Velocity ◽  
Temperature Dependent ◽  
Low K ◽  
Kinetics Of ◽  
External K

A detailed kinetic study of K:Cl cotransport in hyposmotically swollen low K sheep red blood cells was carried out to characterize the nature of the outwardly poised carrier. The kinetic parameters were determined from the rate of K efflux and influx under zero-K-trans conditions in red cells with cellular K altered by the nystatin method and with different extracellular K or Rb concentrations. Although apparent affinities for efflux and influx were quite similar, the maximal velocity for K efflux was approximately two times greater than for influx. Furthermore, at thermodynamic equilibrium (i.e., when the ion product of K and Cl within the cell was equal to that outside) a temperature-dependent net K efflux was observed, approaching zero only when the external product reached approximately two times the internal product. The binding order of the ions to the transporter was asymmetric, being ordered outside (Cl binding first, followed by K) and random inside. K efflux but not influx was trans-inhibited by KCl. Trans inhibition of K efflux was used to verify the order of binding outside: trans inhibition by external Cl occurred in the absence of external K, but not vice versa. Thus K:Cl cotransport is kinetically asymmetric in hyposmotically swollen low K sheep red cells.

Kinetics of O2 uptake and release by red cells in stopped-flow apparatus: Effects of unstirred layer

1985 ◽  
Vol 59 (1) ◽  
pp. 71-91 ◽  
Author(s):  
R.A.B. Holland ◽  
H. Shibata ◽  
P. Scheid ◽  
J. Piiper
Keyword(s):  
Red Cells ◽  
Unstirred Layer ◽  
Stopped Flow ◽  
O2 Uptake ◽  
Flow Apparatus ◽  
Kinetics Of ◽  
Uptake And Release

scholarly journals K-Na EXCHANGE ACCOMPANYING THE PROLYTIC LOSS OF K FROM HUMAN RED CELLS

1947 ◽  
Vol 30 (5) ◽  
pp. 379-387 ◽  
Author(s):  
Eric Ponder
Keyword(s):  
Steady State ◽  
Cell Metabolism ◽  
Ionic Composition ◽  
Surface Membrane ◽  
Sodium Taurocholate ◽  
Red Cells ◽  
Red Cell ◽  
Membrane Changes ◽  
Kinetics Of ◽  
Human Red Cells

In systems containing human red cells and sodium taurocholate as a lysin, or distearyl lecithin as a sphering agent, the prolytic loss of K at 25°C. is accompanied by a gain of Na by the cell, the gain being somewhat greater than the K loss. A small volume increase accompanies the exchange. The kinetics of the K loss and the Na gain are similar to those already described; i.e., the changes are rapid at first, and slow down so that after 12 to 20 hours it appears that a new steady state is being approached. Similar, but smaller, losses of K and gains of Na occur when the cells stand in isotonic NaCl at 25°C. without the addition of a lysin or sphering agent. On these and other experimental grounds, it is impossible to retain the idea that the mammalian red cell in general is impermeable to cations. The cells nevertheless seem to be in a steady state with respect to their environment, their ionic composition changing as the composition of the environment is changed. The possible processes by means of which one steady state can be exchanged for another—changes in the permeability of a surface membrane, changes in the velocity of an active ion transfer process dependent on red cell metabolism, and changes in the activity of the ions in the red cell interior as a result of changes in an orderly internal structure—are discussed.

scholarly journals Affinities or Apparent Affinities of the Transport Adenosine Triphosphatase System

1969 ◽  
Vol 54 (1) ◽  
pp. 289-305 ◽  
Author(s):  
I. M. Glynn ◽  
V. L. Lew
Keyword(s):  
External Medium ◽  
Red Cells ◽  
Potassium Ions ◽  
Potassium Efflux ◽  
High Sodium ◽  
Atpase System ◽  
Review Model ◽  
Free Media ◽  
Recent Experimental Work ◽  
Kinetics Of

The interactions of potassium ions and ATP on transport ATPase activity are discussed, and the interpretation of these interactions is shown to be often ambiguous. Caldwell's (1968) Physiological Review model is discussed with particular reference to the observed kinetics of sodium: sodium exchange in red cells. Recent experimental work on the properties of the ouabain-sensitive component of potassium efflux from red cells is described. This component of efflux occurs only if either sodium or potassium are present in the external medium, but the effects of external sodium and potassium are not additive. The relation between ouabain-sensitive potassium efflux and the external concentration of sodium (in a potassium-free medium) or of potassium (in low- and high-sodium media) are described. When starved sodium-poor red cells are poisoned with iodoacetamide, loaded with phosphate, and incubated in high-sodium potassium-free media, the ouabain-sensitive efflux of potassium appears to be accompanied by the reversal of the entire ATPase system. About two to three potassium ions leave by the ouabain-sensitive route for each molecule of ATP synthesized. If potassium is present in the external medium, no ouabain-sensitive synthesis of ATP occurs and the ouabain-sensitive efflux of potassium presumably involves the reversal of only the last part of the ATPase system.

The response of bovine red cells to freezing and thawing as a function of the permeation of glycerol. I. Kinetics of glycerol permeation

Cryobiology ◽  
1971 ◽  
Vol 8 (4) ◽  
pp. 376 ◽  
Author(s):  
P. Mazur ◽  
R.H. Miller ◽  
S.P. Leibo
Keyword(s):  
Red Cells ◽  
Freezing And Thawing ◽  
Kinetics Of

Kinetics of inhibition of the plasma membrane calcium pump by vanadate in intact human red cells

Cell Calcium ◽  
2001 ◽  
Vol 30 (5) ◽  
pp. 337-342 ◽  
Author(s):  
T. Tiffert ◽  
V.L. Lew
Keyword(s):  
Plasma Membrane ◽  
Red Cells ◽  
Calcium Pump ◽  
Plasma Membrane Calcium Pump ◽  
Kinetics Of ◽  
Kinetics Of Inhibition ◽  
Human Red Cells
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