Rapid Methods and Automation in Microbiology and Immunology

1985 ◽  
Keyword(s):  
Rapid Methods

scholarly journals Utilizing the Food–Pathogen Metabolome to Putatively Identify Biomarkers for the Detection of Shiga Toxin-Producing E. coli (STEC) from Spinach

Metabolites ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 67
Author(s):  
Snehal R. Jadhav ◽  
Rohan M. Shah ◽  
Avinash V. Karpe ◽  
Robert S. Barlow ◽  
Kate E. McMillan ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Metabolic Profiling ◽  
Biomarker Discovery ◽  
Risk Groups ◽  
Proof Of Concept ◽  
Rapid Methods ◽  
E Coli ◽  
Leafy Greens ◽  
Food Pathogen ◽  
Genomic Similarity

Shiga toxigenic E. coli (STEC) are an important cause of foodborne disease globally with many outbreaks linked to the consumption of contaminated foods such as leafy greens. Existing methods for STEC detection and isolation are time-consuming. Rapid methods may assist in preventing contaminated products from reaching consumers. This proof-of-concept study aimed to determine if a metabolomics approach could be used to detect STEC contamination in spinach. Using untargeted metabolic profiling, the bacterial pellets and supernatants arising from bacterial and inoculated spinach enrichments were investigated for the presence of unique metabolites that enabled categorization of three E. coli risk groups. A total of 109 and 471 metabolite features were identified in bacterial and inoculated spinach enrichments, respectively. Supervised OPLS-DA analysis demonstrated clear discrimination between bacterial enrichments containing different risk groups. Further analysis of the spinach enrichments determined that pathogen risk groups 1 and 2 could be easily discriminated from the other groups, though some clustering of risk groups 1 and 2 was observed, likely representing their genomic similarity. Biomarker discovery identified metabolites that were significantly associated with risk groups and may be appropriate targets for potential biosensor development. This study has confirmed that metabolomics can be used to identify the presence of pathogenic E. coli likely to be implicated in human disease.

scholarly journals Rapid methods for antimicrobial resistance diagnosis in contaminated soils for effective remediation strategy

2021 ◽  
Vol 137 ◽  
pp. 116203
Author(s):  
Cailing Zhou ◽  
Yuwei Pan ◽  
Shifu Ge ◽  
Frederic Coulon ◽  
Zhugen Yang
Keyword(s):  
Antimicrobial Resistance ◽  
Contaminated Soils ◽  
Remediation Strategy ◽  
Rapid Methods

scholarly journals Genetic variants of group A apolipoproteins. Rapid methods for screening and characterization without ultracentrifugation.

1982 ◽  
Vol 257 (1) ◽  
pp. 501-507
Author(s):  
G. Utermann ◽  
G. Feussner ◽  
G. Franceschini ◽  
J. Haas ◽  
A. Steinmetz
Keyword(s):  
Genetic Variants ◽  
Rapid Methods ◽  
Group A

Comparative evaluation of five rapid methods for identifying subtype 1b and 2c hepatitis C virus isolates

1997 ◽  
Vol 66 (2) ◽  
pp. 187-194 ◽  
Author(s):  
Marialinda Vatteroni ◽  
Fabrizio Maggi ◽  
Antonietta Morrica ◽  
Claudia Fornai ◽  
Massimo Giorgi ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Comparative Evaluation ◽  
Rapid Methods ◽  
Subtype 1B ◽  
Virus Isolates
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