Genetic variants of group A apolipoproteins. Rapid methods for screening and characterization without ultracentrifugation.

El rendimiento físico se ha asociado con diferentes variantes genéticas. El objetivo de este estudio fue determinar la asociación entre características dermatoglíficas y los genotipos ACTN3 y ECA. La muestra la constituyen 82 seleccionados nacionales de diferentes modalidades deportivas de Brasil, Japón y Chile. Los marcadores ACTN3 y ECA se obtuvieron a través de una muestra de saliva y se analizaron mediante el empleo de cadena de la polimerasa en tiempo real a partir del iQ5ThermalCycler, BioRad, mientras que para la configuración dermatoglifica se utilizó un lector de huella digital Verifier® 320 LC 2.0. Estos deportistas fueron clasificados en grupos de acuerdo a la configuración de sus patrones dermatoglíficos (A, L, W, D10 y SQTL) en los siguientes grupos: aeróbicos (n= 27); anaeróbicos (n= 55). En cuanto a la frecuencia de aparición de polimorfismos, para ambos grupos predomina el genotipo RX 48,0% y 49,1% de ACTN3, y DI 68,0% y 41,3% para ECA, en los grupos aeróbico y anaeróbico respectivamente. En el grupo aeróbico se observa una asociación muy alta entre ACTN3 con presilla, verticilo y D10 (r=0,90), en el grupo anaeróbico solo observa asociación alta en presilla (r=0,77), para el gen ECA se observan asociaciones moderadas entre presilla, verticilo y D10 (r=0,45), en el grupo aeróbico. Conclusión: Las características dermatoglíficas pueden estar asociados con la variante alélica del gen ACTN3 (RR) y ECA (DI), para perfiles deportivos de carácter anaeróbico. Abstract. Physical performance has been associated with different genetic variants. The objective of this study was to determine the association between dermatoglyphic characteristics and the ACTN3 and ECA genotypes. The sample is established by 82 national teams from different sports modalities in Brazil, Japan and Chile. The ACTN3 and ECA markers were obtained through a saliva sample and was analyzed using the polymerase chain in real time from the iQ5ThermalCycler, BioRad, while a Verifier® 320 LC fingerprint reader was used for the dermatoglyphic configuration. These athletes were classified into groups according to the configuration of their dermatoglyphic patterns (A, L, W, D10 and SQTL) in the following groups: aerobics (n = 27); anaerobic (n = 55). Regarding the frequency in the appearance of polymorphisms, the RX genotype 48.0% and 49.1% of ACTN3 predominated for both groups and DI 68.0% and 41.3% for ACE, in the aerobic and anaerobic groups respectively. In the aerobic group a very high association is observed between ACTN3 with a clip, verticil and D10 (r = 0.90), in the anaerobic group only a high association was observed in the clip (r = 0.77), for the ACE gene they are observed moderate associations between clip, verticil and D10 (r = 0.45), in the aerobic group. Conclusion: Dermatoglyphic characteristics may be associated with the allelic variant of the ACTN3 (RR) and RCT (DI) gene, for sports profiles of an anaerobic nature.

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Electron microscopic radioautographic studies on the incorporation of 3H proline in the glomerular basement membrane (GBM) in antistreptococcal-cell-membrane (SCM) injected mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100111057 ◽

1984 ◽

Vol 42 ◽

pp. 188-189

Author(s):

R.P. Nayyar ◽

C.F. Lange ◽

J. L. Borke

Keyword(s):

Body Weight ◽

Cell Membrane ◽

Basement Membrane ◽

Glomerular Basement Membrane ◽

Mesangial Matrix ◽

Electron Microscopic ◽

Group A ◽

Injection Protocol

Streptococcal cell membrane (SCM) antiserum injected mice show a significant thickening of glomerular basement membrane (GBM) and an increase in mesangial matrix within 4 to 24 hours of antiserum administration (1,2,3). This study was undertaken to evaluate the incorporation of 3H proline into glomerular cells and GBM under normal and anti-SCM induced conditions. Mice were administered, intraperitoneally, 0.1 ml of normal or anti-SCM serum followed by a 10 µC/g body weight injection of 3H proline. Details of the preparation of anti-SCM (Group A type 12 streptococcal pyogenes) and other sera and injection protocol have been described elsewhere (2). After 15 minutes of isotope injection a chase of cold proline was given and animal sacrificed at 20 minutes, 1,2,4,8,24 and 48 hours. One of the removed kidneys was processed for immunofluorescence, light and electron microscopic radioautographic studies; second kidney was used for GBM isolation and aminoacid analysis.

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An ultrastructural study of sertoli cells in two geographically isolated populations of Aphanius dispar

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100123143 ◽

1992 ◽

Vol 50 (1) ◽

pp. 548-549

Author(s):

Taber A. Ba-Omar ◽

Philip F. Prentis

Keyword(s):

Ultrastructural Level ◽

Uranyl Acetate ◽

Freshwater Teleost ◽

Isolated Populations ◽

En Bloc ◽

The North ◽

Group A ◽

Ultrathin Sections ◽

Group B ◽

Geographically Isolated

We have recently carried out a study of spermiogenic differentiation in two geographically isolated populations of Aphanius dispar (freshwater teleost), with a view to ascertaining variation at the ultrastructural level. The sampling areas were the Jebel Al Akhdar in the north (Group A) and the Dhofar region (Group B) in the south. Specimens from each group were collected, the testes removed, fixed in Karnovsky solution, post fixed in OsO, en bloc stained with uranyl acetate and then routinely processed to Agar 100 resin, semi and ultrathin sections were prepared for study.

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Development of research manpower for dentistry: consensus report, Group A

Journal of Dental Education ◽

10.1002/j.0022-0337.1969.33.1.tb00144.x ◽

1969 ◽

Vol 33 (1) ◽

pp. 17-20

Author(s):

JL Henry ◽

RE McDonald

Keyword(s):

Group A ◽

Consensus Report

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Cultural and Linguistic Adaptation of the Consensus Auditory-Perceptual Evaluation of Voice (CAPE-V) Into Hindi

Journal of Speech Language and Hearing Research ◽

10.1044/2020_jslhr-20-00348 ◽

2020 ◽

Vol 63 (12) ◽

pp. 3974-3981

Author(s):

Ashwini Joshi ◽

Isha Baheti ◽

Vrushali Angadi

Keyword(s):

Strong Correlation ◽

Concurrent Validity ◽

Interrater Reliability ◽

Voice Quality ◽

Weak Correlation ◽

Voice Assessment ◽

Perceptual Evaluation ◽

Severity Grade ◽

Normal Voice ◽

Group A

Aim The purpose of this study was to develop and assess the reliability of a Hindi version of the Consensus Auditory-Perceptual Evaluation of Voice (CAPE-V). Reliability was assessed by comparing Hindi CAPE-V ratings with English CAPE-V ratings and by the Grade, Roughness, Breathiness, Asthenia and Strain (GRBAS) scale. Method Hindi sentences were created to match the phonemic load of the corresponding English CAPE-V sentences. The Hindi sentences were adapted for linguistic content. The original English and adapted Hindi CAPE-V and GRBAS were completed for 33 bilingual individuals with normal voice quality. Additionally, the Hindi CAPE-V and GRBAS were completed for 13 Hindi speakers with disordered voice quality. The agreement of CAPE-V ratings was assessed between language versions, GRBAS ratings, and two rater pairs (three raters in total). Pearson product–moment correlation was completed for all comparisons. Results A strong correlation ( r > .8, p < .01) was found between the Hindi CAPE-V scores and the English CAPE-V scores for most variables in normal voice participants. A weak correlation was found for the variable of strain ( r < .2, p = .400) in the normative group. A strong correlation ( r > .6, p < .01) was found between the overall severity/grade, roughness, and breathiness scores in the GRBAS scale and the CAPE-V scale in normal and disordered voice samples. Significant interrater reliability ( r > .75) was present in overall severity and breathiness. Conclusions The Hindi version of the CAPE-V demonstrates good interrater reliability and concurrent validity with the English CAPE-V and the GRBAS. The Hindi CAPE-V can be used for the auditory-perceptual voice assessment of Hindi speakers.

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A Test of Satiation as a Function of Adaptation in Stuttering

Journal of Speech and Hearing Research ◽

10.1044/jshr.1201.110 ◽

1969 ◽

Vol 12 (1) ◽

pp. 110-117 ◽

Cited By ~ 2

Author(s):

Harold A. Peterson ◽

Mary Beth Rieck ◽

Rita K. Hoff

Keyword(s):

Semantic Differential ◽

Repeated Readings ◽

Group A ◽

Relationship Of ◽

The Relationship

To test the relationship of adaptation and satiation as hypothesized by Jakobovits, satiation of meaning as a function of repeated readings for adaptation was measured in the performance of 14 male stutterers. The subjects as a group exhibited both satiation and adaptation, but the two phenomena did not occur simultaneously in a significant number of the members of the group. A reduction in meaningfulness, as measured by the semantic differential, was not shown to be a significant factor in the reduction of stuttering frequency for the individuals in the group. Satiation and adaptation were not established as the same phenomenon, although the two may still be related through another factor.

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