Ultrastructural Localization of Epithelial Membrane Antigen and Carcinoembryonic Antigen in Malignant Mesothelial and Epithelial Cells

The ultrastructural localization of deoxyribonucleic acid (DNA) synthesis was studied by electron-microscope autoradiography in human transforming lymphocytes, embryonic lung fibroblasts, epithelial cells and normoblasts. Euchromatin was found to be active in DNA synthesis in all cell types studied, whereas heterochromatin was inactive. However, DNA synthesis was also prominent in the regions where heterochromatin was thought to be decondensing to form euchromatin. Analysis of sequential changes in nuclear morphology of the transforming lymphocyte suggested that there is decondensation of heterochromatin during the S-phase until none is left. In nuclei with no heterochromatin a prominent localization of DNA synthesis was at the nuclear membrane. This sequence of complete decondensation of heterochromatin also seemed likely for fibroblasts and epithelial cells. Normoblasts however showed no stage where the nucleus was wholly euchromatic and it is suggested that in this cell decondensation of heterochromatin for replication is localized and transient.

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The distinction of adenocarcinoma from malignant mesothelioma in cell blocks of effusions: The role of routine mucin histochemistry and immunohistochemical assessment of carcinoembryonic antigen, keratin proteins, epithelial membrane antigen, and milk fat globule-derived antigen

Lung Cancer ◽

10.1016/s0169-5002(87)80181-2 ◽

1987 ◽

Vol 3 (3-4) ◽

pp. 137

Keyword(s):

Carcinoembryonic Antigen ◽

Malignant Mesothelioma ◽

Milk Fat ◽

Epithelial Membrane Antigen ◽

Mucin Histochemistry ◽

Milk Fat Globule ◽

Immunohistochemical Assessment ◽

Fat Globule ◽

Keratin Proteins

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Paget's Disease in Gynecomastia: Immunohistochemical Study of a Case

Tumori Journal ◽

10.1177/030089168807400211 ◽

1988 ◽

Vol 74 (2) ◽

pp. 183-190 ◽

Cited By ~ 6

Author(s):

Pietro Muretto ◽

Vincenzo Polizzi ◽

M. Pia Staccioli

Keyword(s):

Breast Carcinoma ◽

Carcinoembryonic Antigen ◽

Epithelial Membrane Antigen ◽

Immunohistochemical Study ◽

Paget’S Disease ◽

Paget's Disease ◽

Male Breast ◽

Intraductal Carcinoma ◽

Review Of The Literature ◽

Good Tool

A case of Paget's disease and gynecomastia in a 70-year-old man is reported. Paget's disease was connected to an intraductal carcinoma, and the immunohistochemical study revealed similar positivity for cytokeratin A, carcinoembryonic antigen and epithelial membrane antigen in Paget cells and intraductal neoplastic cells whereas Paget cells resulted negative for cytokeratin B and C. The study using monoclonal anti-cytokeratin A (35 βH11), B (34 βE12) and C (34 βB4) could represent a good tool, supporting the theory of a ductal origin of Paget cells. A review of the literature has shown the rarity of Paget's disease in the male breast and revealed only two previous reports with an associated gynecomastia, in 2 patients with Klinefelter's syndrome and infiltrating breast carcinoma.

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