Effect of Cardiac Glycosides on Active Na-K-Transport

Abstract Monovalent ions are involved in growth, proliferation, differentiation of cells as well as in their death. This work concerns the ion homeostasis during senescence induction in human mesenchymal endometrium stem cells (hMESC): hMESCs subjected to oxidative stress (pulse H2O2 treatment) enter the premature senescence accompanied by persistent DNA damage, irreversible cell cycle arrest, cell hypertrophy, lipofuscin accumulation, enhanced β-galactosidase activity. Using flame photometry to estimate K+, Na+ content and Rb+ (K+) fluxes we found that during the senescence development in stress-induced hMESCs, Na+/K+pump-mediated K+ fluxes are enhanced due to the increased Na+ content in senescent cells, while ouabain-resistant K+ fluxes remain unchanged. Senescence progression is accompanied by a peculiar decrease in the K+ content in cells from 800-900 µmol/g to 500-600 µmol/g. Since cardiac glycosides are offered as selective agents for eliminating senescent cells, we investigated the effect of ouabain on ion homeostasis and viability of hMESCs and found that in both proliferating and senescent hMESCs, ouabain (1 nM-1 µM, 24-48 h) inhibited pump-mediated K+ transport (ID50 5x10-8 M), decreased cell K+/Na+ ratio to 0,1-0,2, however did not induce apoptosis. Comparison of the effect of ouabain on hMESCs with the literature data on the selective cytotoxic effect of cardiac glycosides on senescent or cancer cells suggests the ion pump blockade and intracellular K+ depletion should be synergized with target apoptotic signal to induce the cell death.

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A New Concept of Organization of the Central Emetic Mechanism: Recent Studies on the Sites of Action of Apomorphine, Copper Sulfate And Cardiac Glycosides

Gastroenterology ◽

10.1016/s0016-5085(19)36357-7 ◽

1952 ◽

Vol 22 (1) ◽

pp. 1-12 ◽

Cited By ~ 167

Author(s):

S.C. Wang ◽

Herbert L. Borison

Keyword(s):

Copper Sulfate ◽

Cardiac Glycosides ◽

Sites Of Action

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Characterization of non polar-polar solvent extracts from some tea plantation shade tree leaves with special reference to antioxidant and antibacterial activities

Research in Plant Biology ◽

10.25081/ripb.2020.v10.6235 ◽

2020 ◽

pp. 15-21

Author(s):

Arindam Ghosh ◽

Soumya Majumder ◽

Sumedha Saha ◽

Malay Bhattacharya

Keyword(s):

Cardiac Glycosides ◽

Polar Solvent ◽

Reducing Power ◽

Scavenging Activity ◽

Shade Trees ◽

Crude Fibre ◽

Shade Tree ◽

Dpph Scavenging Activity ◽

Dpph Scavenging

Beneficial properties of shade trees of tea plantations other than their medicinal properties have been extensively studied. This research was initiated to explore the properties of some shade trees with special emphasis on their antioxidant and antibacterial properties. Leaves from shade tree like Dalbergia sissoo (DS), Cassia siamea (CS), Derris robusta (DR), Leucaena leucocephala (LL), Acacia lenticularis (AL) and Melia azedarach (MA) were used for the study. Characterization of shade tree leaves by determination of moisture, crude fibre and ash content and tests of non polar – polar solvent extracts for steroid, tannins, cardiac glycosides and coumarin, free radical scavenging, ferric reducing power, NO scavenging activities, quantification of Flavonoids and antibacterial activity were conducted. The average moisture, crude fibre and ash percentage of shade tree plants were found to be 62.95, 11.28 and 1.86 respectively. Methanol, ethanol, acetone and ethyl acetate respectively proved to be the most potent solvent for various phytochemical extractions as it gave positive results for tests like tannin, steroid, cardiac glycosides and coumarin. AL (91.46%), DR (92.69%), LL (94.32%) and MA (93.34%) leaf extracts showed a high level of DPPH scavenging activity in their water extracts. In DS (88.11%) and CS (83.23%) maximum DPPH scavenging activity was observed in Diethyl ether and Methanol extracts respectively. Acetone extracts were more active than the water extracts in exhibiting ferric reducing power and NO scavenging activity. Summation of the quantity revealed that DS showed maximum presence of flavonoids and acetone as most potential for isolation of flavonoids. The decreasing order of summative antibacterial activity was recorded in DS, followed by CS, DR, AL, MA and LL. Chloroform showed the highest summative inhibition zone followed by ethanol, ethyl acetate, diethyl ether, acetone, water, hexane, benzene and methanol. The antioxidant and antibacterial potential of shade trees were established.

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Investigation of antiulcer activity of Pergularia extensa Linn

International Journal of Research in Phytochemistry and Pharmacology ◽

10.26452/ijrpp.v9i2.1198 ◽

2020 ◽

Vol 9 (2) ◽

pp. 23-26

Author(s):

Pavani C H

Keyword(s):

Cardiac Glycosides ◽

Methanol Extract ◽

Methanolic Extract ◽

Antiulcer Activity ◽

Acute Oral Toxicity ◽

Gastric Lesions ◽

Oral Toxicity ◽

Control Groups ◽

Dose Dependent

This study was based on determination of the antiulcer activity from methanol extract was prepared by using barks of pergularia extensa linn.. Priliminary investigations showed presence of saponins, terpenes, cardiac glycosides, alkaloids and sterols. Based on OECD-423 Guidelines, the pharmacology and acute oral toxicity studies were conducted by using methanolic extract. Ulcer development was prevented by Tannins because of their vasoconstriction effects and due to protein precipitation. Similarly, the Methanolic extract of Pergularia extensa Linn shows triterpenoids and saponins. The phytoconstituents are present in the extract and these could be possible agents which are involved in order to prevent gastric lesions induced by aspirin. When compared to ulcerative control groups, this Pergularia extensa Linn., shows a dose dependent curative ratio. The extracts exhibited an inhibition percentage of 27.18, 45.47 and 61.28 at doses of 100, 200 and 400mg/kg doses respectively.

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