In Vitro Adherence of Candida albicans to Acrylic Resin with Different Surface Status

SummaryBackground/Aim: Although there are studies about the antimicrobial activity of silver, there is no study evaluating it as a denture disinfectant. The purpose of this study was to explore the effectiveness of 6 disinfectant solutions (50% vinegar, 100% vinegar, 1% silver nitrate, 2% silver nitrate, %1 sodium hypochlorite, 0,12% chlorhexidine digluconate) in the disinfection of acrylic resin specimens contaminated in vitro by Candida albicans, as measured by residual colony-forming unit (CFU).Material and Methods: 66 pieces of 10mmx2mm acrylic resin disc samples were prepared and incubated in 1x106 cell/ml suspension of C. albicans ATCC 18804 for 24 h (one of them as a control, n=11/group). The specimens were then transferred into tubes containing 10 ml of the tested disinfectants and kept for 10 minutes in the disinfectant. After washing with saline, the specimens were vortexed to remove the microorganisms adhered to the surfaces. Colony counting of the collected microorganisms was performed on Sabouroud dextrose medium using 10−1, 10−2 and 10−3 dilutions. The results were analysed using Kruskal-Wallis and Mann-Whitney U tests (p<0,05).Results: The results showed that 1% sodium hypochlorite, %1 silver nitrate and 2% silver nitrate were the most effective against Candida Albicans (p<0,05), followed by 100% vinegar, 0,12% chlorhexidine digluconate and 50% vinegar (p<0,05).Conclusions: Within the boundaries of this study, we conclude that 1% silver nitrate is a promising alternative disinfectant to 1% sodium hypochlorite and performs better compared to 0,12 % chlorhexidine gluconate, 50% and 100% vinegar.

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Effectiveness of mechanical brushing with different denture cleansing agents in reducing in vitro Candida albicans biofilm viability

Brazilian Dental Journal ◽

10.1590/s0103-64402012000500013 ◽

2012 ◽

Vol 23 (5) ◽

pp. 547-554 ◽

Cited By ~ 16

Author(s):

Delise Pellizzaro ◽

Gregory Polyzois ◽

Ana Lucia Machado ◽

Eunice Teresinha Giampaolo ◽

Paula Volpato Sanitá ◽

...

Keyword(s):

Candida Albicans ◽

Biofilm Formation ◽

Reduction Method ◽

Acrylic Resin ◽

Chlorhexidine Gluconate ◽

Common Disease ◽

Combined Method ◽

Denture Stomatitis ◽

Positive Controls

The adhesion of Candida albicans to surfaces is the prerequisite for occurrence of denture stomatitis, a common disease diagnosed among denture wearers. A routine of denture cleansing is essential to prevent biofilm formation and the onset of this infection. The aim of this study was to investigate the effectiveness of combining brushing and cleansing agents in killing C. albicans biofilm. Disks of acrylic resin were made, sterilized, and inoculated with C. albicans (10(7) cfu/mL). After incubation (37°C/48 h), specimens were randomly assigned to 10 experimental groups (n=9): 5 subjected to brushing with distilled water or cleansing agents - dentifrice slurry, 2% chlorhexidine gluconate (CHX), 1% sodium hypochlorite (NaOCl), and Polident fresh cleanse® (combined method) - and 4 exposed to the cleansing agents without brushing (immersion). Non-cleansed specimens were used as positive controls. The viability of cells was evaluated by XTT reduction method. Results were analyzed by Mann-Whitney and Kruskal-Wallis tests (α=0.05). The combined method was significantly more effective (p<0.0001) in reducing biofilm viability than the immersion. Brushing with CHX and NaOCl resulted in 100% removal of the biofilm. Immersion in the agents reduced significantly (p<0.0001) the biofilm viability, with CHX being the most effective (p<0.0001). The use of the combined method of brushing with cleansing agents is an effective method to reduce C. albicans biofilm, being CHX and NaOCl the most effective solutions.

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Effect of Denture Base Fabrication Technique on Candida albicans Adhesion In Vitro

Materials ◽

10.3390/ma14010221 ◽

2021 ◽

Vol 14 (1) ◽

pp. 221

Author(s):

Avi Meirowitz ◽

Arkadi Rahmanov ◽

Eti Shlomo ◽

Helena Zelikman ◽

Eran Dolev ◽

...

Keyword(s):

Surface Roughness ◽

Candida Albicans ◽

3D Printing ◽

Oral Candidiasis ◽

Acrylic Resin ◽

Denture Base ◽

Denture Stomatitis ◽

Heat Curing ◽

Vitro Effect

Denture stomatitis is a common manifestation of oral candidiasis affecting some 65% of denture wearers. This condition is initiated by the adherence of Candida albicans to denture base acrylic resin. The present study aimed to test the in vitro effect of traditional and novel fabrication methods on Candida albicans adhesion to denture base samples. Denture based acrylic discs were fabricated using: (i) computerized milling, (ii) 3D printing, (iii) heat curing, and (iv) cold curing. Discs were tested for surface roughness (Ra), hydrophobicity (contact angle), mucin adsorption (Bradford assay), and Candida albicans adhesion. 3D printing significantly increased microbial cell adhesion as compared with heat curing, and computerized milling significantly decreased it. These results were associated with mucin adsorption levels rather than surface roughness. Results suggest that 3D printing may increase the risk for developing denture stomatitis, whereas computerized milling may decrease it as compared with traditional heat curing denture base fabrication.

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Effect of Bamboo Salt on Inhibition of Adhesion of Candida albicans to Denture Acrylic Resin: An in vitro Study

JOURNAL FOR CLINICAL AND DIAGNOSTIC RESEARCH ◽

10.7860/jcdr/2018/30315.11102 ◽

2018 ◽

Author(s):

Keerthika Natarajan ◽

Murugesan Krishnan ◽

Suganya Srinivasan ◽

Gowtham Venkat ◽

Muthukumar Balasubramaniam

Keyword(s):

Candida Albicans ◽

In Vitro Study ◽

Acrylic Resin ◽

Vitro Study ◽

Bamboo Salt

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An in vitro study on effect of Delmopinol application on Candida albicans adherence on heat cured denture base acrylic resin: A thorough study

Indian Journal of Dental Research ◽

10.4103/0970-9290.123423 ◽

2013 ◽

Vol 24 (5) ◽

pp. 645 ◽

Cited By ~ 4

Author(s):

Deshraj Jain ◽

Prabha Shakya

Keyword(s):

Candida Albicans ◽

In Vitro Study ◽

Acrylic Resin ◽

Vitro Study ◽

Denture Base

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An in vitro and in vivo study of the effect of incorporation of chlorhexidine into autopolymerizing acrylic resin plates upon the growth of Candida albicans

Biomaterials ◽

10.1016/0142-9612(83)90012-1 ◽

1983 ◽

Vol 4 (3) ◽

pp. 205-209 ◽

Cited By ~ 27

Author(s):

D.J. Lamb ◽

M.V. Martin

Keyword(s):

Candida Albicans ◽

Acrylic Resin ◽

In Vivo Study

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In Vitro Antifungal Evaluation of Seven Different Disinfectants on Acrylic Resins

BioMed Research International ◽

10.1155/2014/519098 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 7

Author(s):

A. Z. Yildirim-Bicer ◽

I. Peker ◽

G. Akca ◽

I. Celik

Keyword(s):

Surface Roughness ◽

Candida Albicans ◽

Uv Light ◽

Acrylic Resin ◽

Cost Effective ◽

Alternative Methods ◽

Acrylic Resins ◽

Significant Difference ◽

White Vinegar

Objective. The aim of this study was to evaluate alternative methods for the disinfection of denture-based materials.Material and Methods. Two different denture-based materials were included in the study. Before microbial test, the surface roughness of the acrylic resins was evaluated. Then, the specimens were divided into 8 experimental groups(n=10), according to microorganism considered and disinfection methods used. The specimens were contaminated in vitro by standardized suspensions ofCandida albicansATCC#90028 andCandida albicansoral isolate. The following test agents were tested: sodium hypochlorite (NaOCl 1%), microwave (MW) energy, ultraviolet (UV) light, mouthwash containing propolis (MCP), Corega Tabs, 50% and 100% white vinegar. After the disinfection procedure, the number of remaining microbial cells was evaluated in CFU/mL. Kruskal-Wallis, ANOVA, and Dunn’s test were used for multiple comparisons. Mann WhitneyUtest was used to compare the surface roughness.Results. Statistically significant difference(P<0.05)was found between autopolymerised and heat-cured acrylic resins. The autopolymerised acrylic resin surfaces were rougher than surfaces of heat-cured acrylic resin. The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins.Conclusion. This study showed that white vinegar 100% was the most effective method for tested microorganisms. This agent is cost-effective and easy to access and thus may be appropriate for household use.

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