Mild Hypothermia Amelioration of Damage During Rat Spinal Cord Injury: Inhibition of Pathological Microglial Proliferation and Improvement of Hind-limb Motor Function

2000 ◽  
pp. 47-54
Author(s):  
Tadanori Ogata ◽  
Tadao Morino ◽  
Jun Takeba ◽  
Yoshiro Matsuda ◽  
Hideo Okumura ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Motor Function ◽  
Hind Limb ◽  
Mild Hypothermia ◽  
Rat Spinal Cord ◽  
Cord Injury ◽  
Microglial Proliferation

scholarly journals Stable Gastric Pentadecapeptide BPC 157 Recovers Motor Function After Rat Spinal Cord Injury

2019 ◽  
Vol 33 (S1) ◽  
Author(s):  
Darko Perovic ◽  
Ivan Krezic ◽  
Stjepan Dokuzovic ◽  
Vide Bilic ◽  
Slaven Gojkovic ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Motor Function ◽  
Rat Spinal Cord ◽  
Bpc 157 ◽  
Cord Injury ◽  
Pentadecapeptide Bpc 157

scholarly journals Zhenbao pill protects against acute spinal cord injury via miR-146a-5p regulating the expression of GPR17

2018 ◽  
Vol 38 (1) ◽  
Author(s):  
Yongxiong He ◽  
Bokang Lv ◽  
Yanqiang Huan ◽  
Bin Liu ◽  
Yutang Li ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Motor Function ◽  
Hind Limb ◽  
Neuronal Apoptosis ◽  
Acute Spinal Cord Injury ◽  
Luciferase Reporter Assay ◽  
Luciferase Reporter ◽  
Reporter Assay ◽  
Cord Injury

The aim of the present study was to observe the effect of zhenbao pill on the motor function of acute spinal cord injury (ASCI) rats and the molecular mechanisms involving miR-146a-5p and G-protein-coupled receptor 17 (GPR17). ASCI rat model was established by modified Allen method, and then the rats were divided into three groups. SH-SY5Y cells were cultured overnight in hypoxia condition and transfected with miR-146a-5p mimic or miR-146a-5p inhibitor. The hind limb motor function of the rats was evaluated by Basso, Beattie, Bresnahan (BBB) scoring system. Quantitative real-time PCR (qRT-PCR) and Western blot were used to detect the expression of miR-146a-5p, GPR17, inducible nitric oxide synthase (iNOS), interleukin 1β (IL-1β), and tumor necrosis factor α (TNF-α). Neuronal apoptosis was measured using flow cytometry assay. Luciferase reporter assay was performed to determine the regulation of miR-146a-5p on GPR17. Zhenbao pill could enhance hind limb motor function and attenuate the inflammatory response caused by ASCI. Moreover, zhenbao pill increased the level of miR-146a-5p and decreased GPR17 expression in vivo and in vitro. Bioinformatics software predicted that GPR17 3′-UTR had a binding site with miR-146a-5p. Luciferase reporter assay showed that miR-146a-5p had a negative regulatory effect on GPR17 expression. Knockdown of miR-146a-5p could reverse the effect of zhenbao pill on the up-regulation of GPR17 induced by hypoxia, reversed the inhibitory effect of zhenbao pill on the cell apoptosis induced by hypoxia and the recovery of zhenbao pill on hind limb motor function in ASCI rats. Zhenbao pill could inhibit neuronal apoptosis by regulating miR-146a-5p/GPR17 expression, and then promoting the recovery of spinal cord function.

6-Shogaol, a natural product, reduces cell death and restores motor function in rat spinal cord injury

2006 ◽  
Vol 24 (4) ◽  
pp. 1042-1052 ◽  
Author(s):  
Kang Soo Kyung ◽  
Jeon Hyo Gon ◽  
Kim Yong Geun ◽  
Jung Jin Sup ◽  
Woo Jae Suk ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Death ◽  
Natural Product ◽  
Motor Function ◽  
Rat Spinal Cord ◽  
Cord Injury

scholarly journals Effect of fetal cerebellar tissue transplantation on the restoration of hind limb locomotor function in rats with spinal cord injury

2016 ◽  
Vol 4 (2) ◽  
pp. 175-180
Author(s):  
V. Medvediev ◽  
Yu. Senchyk ◽  
N. Draguntsova ◽  
S. Dychko ◽  
V. Tsymbaliuk
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Motor Function ◽  
Hind Limb ◽  
Tissue Transplantation ◽  
Control Group ◽  
Function Recovery ◽  
Cord Injury ◽  
Cerebellar Tissue ◽  
Positive Effect

Fetal cerebellar tissue contains the largest number of neurogenic progenitors committed on the differentiation into glutamatergic neurons that can be used in the development of promising new treatment for spinal cord injuries.To evaluate the effect of fetal cerebellar tissue transplantation (FСTT) on the restoration of motor function after spinal cord injury in experiment.Materials and methods. Animals: inbred albino Wistar rats (5.5 months males, weighting 300 grams); main experimental groups: 1 – spinal cord injury + transplantation of a fragment of fetal (E18) rat cerebellum (n = 15), 2 – spinal cord injury only (n = 40). Model of an injury – left-side spinal cord hemisection at Т11; monitoring of the ipsilateral hind limb function (IHLF) – the Вasso-Вeattie-Вresnahan (BBB) scale.Results. FСTT normalizes the distribution of IHLF values, distorts the dynamics of the motor function recovery, transforming it from a progressive (in a control group) to the constant with variation within 3-3.6 points BBB during the experiment. FСTT causes early temporary positive effect on the functional state of the motor system, probably provided by mediator-dependent, neuroprotective, proangiogenic effect and remyelination. In our view, the gradual depletion of the FСTT positive effect due to resorption of the graft within the first 2 months is compensated by autoregenerative neoplastic process that is typical for the control group and by autoimmune utilization of myelin-associated inhibitors of axonal growth in the zone of injury that causes stability of the IHLF value during the observation period.Conclusion. Transplantation of fetal cerebellar tissue causes a short-term positive effect on the motor function recovery limited by the 1st month of the traumatic process. Evaluation of such type of neurotransplantation effectiveness requires taking into account the dynamics of the spasticity and chronic pain.

scholarly journals Study on Paeoniflorin Promoting the Recovery of Motor Function of Rat Spinal Cord Injury by Mediating MAPK/ERK and Akt/mTOR Signal Pathway

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Pei Zhang ◽  
Nan Wu ◽  
Zhijun Song ◽  
Zhengfu Tai
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Protein Expression ◽  
Motor Function ◽  
Neuroprotective Effect ◽  
Expression Level ◽  
Signal Pathways ◽  
Rat Spinal Cord ◽  
Traditional Chinese Herbal Medicine ◽  
Cord Injury

Spinal cord injury (SCI) is a devastating disease that can cause severe motor, sensory, and autonomic dysfunction. There is currently no effective treatment. Paeonia lactiflora is a traditional Chinese herbal medicine, which has antispasmodic, analgesic, and blood circulation effects. Paeoniflorin (PEF) is a medicinal plant isolated from Paeoniae Radio, and it is widely used in East Asia. A large number of studies have shown that PEF has a powerful neuroprotective effect. However, the potential mechanism of PEF on SCI needs further study. This project uses Basso Beattie Bresnahan (BBB) motor function score and open field test to evaluate neurological function, and uses immunofluorescence method to detect brain-derived neurotrophic factor (BNDF) and neurotrophin-3 (NT). -3) Protein expression, Western blot is used to detect protein expression level, and RT-PCR is used to detect mRNA expression level. Thus, a rat spinal cord injury model was established to observe the effects of AT/mTOR and MAPK/ERK signal pathways activated by PEF on nerve regeneration and functional recovery in rats with spinal cord injury.

scholarly journals Neuroprotective Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells From Different Donors on Spinal Cord Injury in Mice

2022 ◽  
Vol 15 ◽  
Author(s):  
Xu Zhu ◽  
Zhen Wang ◽  
Yi Eve Sun ◽  
Yuchen Liu ◽  
Zhourui Wu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Cell Proliferation ◽  
Inflammatory Response ◽  
Nerve Injury ◽  
Motor Function ◽  
Hind Limb ◽  
Human Umbilical Cord ◽  
Qrt Pcr ◽  
Cord Injury

Spinal cord injury (SCI) is caused by an external force, leading to severe dysfunction of the limbs below the injured segment. The inflammatory response plays a vital role in the prognosis of SCI. Human umbilical cord mesenchymal stem cell (hUCMSC) transplantation can promote repair of SCI by reducing the inflammatory response. We previously showed that hUCMSCs from 32 donors had different inhibitory abilities on BV2 cell proliferation. In this study, three experimental groups were established, and the mice were injected with different lines of hUCMSCs. Hind limb motor function, hematoxylin-eosin (H&E) staining, immunohistochemistry, Western blot (WB), qualitative real-time polymerase chain reaction (qRT-PCR), and RNA sequencing and correlation analysis were used to investigate the effects of hUCMSC transplantation on SCI mice and the underlying mechanisms. The results showed that the therapeutic effects of the three hUCMSC lines were positively correlated with their inhibitory abilities of BV2 cell proliferation rates in vitro. The MSC_A line had a better therapeutic effect on improving the hind limb motor function and greater effect on reducing the expression of glial fibrillary acidic protein (Gfap) and ionized calcium binding adaptor molecule 1 (Iba1) and increasing the expression of neuronal nuclei (NeuN). Differentially expressed genes including Zbtb16, Per3, and Hif3a were probably the key genes involved in the protective mechanism by MSC_A after nerve injury. qRT-PCR results further verified that Zbtb16, Per3, and Hif3a expressions reduced by SCI could be reversed by MSC_A application. These results suggest that the effect of hUCMSCs transplantation on acute SCI depends on their inhibitory abilities to inflammation reaction after nerve injury, which may help to shape future use of hUCMSCs combined with improving the effectiveness of clinical transformation.

scholarly journals Pannexin-1 Characterization after Spinal Cord Injury in Rats

2020 ◽  
Author(s):  
Yu Huang ◽  
Jin Lin ◽  
Xuanwei Chen ◽  
Jianhua Lin
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Western Blot ◽  
Real Time ◽  
Motor Function ◽  
Real Time Pcr ◽  
Hind Limb ◽  
Pannexin 1 ◽  
Cord Injury

Abstract Background Pannexin-1 (Panx-1) is a homomeric membrane semi-channel mostly expressed in the central nervous system of mammals, including neurons and glial cells. Panx-1 channels are highly permeable to calcium and Adenosine Triphosphatase (ATP), it plays an important role in Hypoxic injury of cerebral ischemia through a variety of signal pathways, nerve cell apoptosis and inflammatory response. However, its specific role in spinal cord injury (SCI) is not clear. In the current study, we aimed to investigate the characterization of Panx-1 after SCI in rats, and further analysis were made on its effect and possible mechanism in SCI in order to provide the experimental evidence for potential interfering target spot on SCI therapy. Methods A rat SCI model (Allen’s model) was established by NYU Impactor-III and the hind limb motor function of rats was observed by BBB score. The expression of Panx-1 was detected by Real-time PCR and Western Blot. The correlation between Panx-1 expression and the BBB score in rats after SCI was analyzed to reveal the role of Panx-1 in SCI. Results We found that the BBB score gradually recovered after SCI, but declined significantly at day 2 after SCI. Real-time PCR and Western Blot detection showed that compared with the normal control group and the sham operation group, the expression of Panx-1 increased significantly with time after SCI, and reached a peak at day 2 after SCI. Moreover, there was a significant negative correlation between the expression of Panx-1 protein and the BBB score of rat hind limb motor function at day 1, day 2, day 3 and day 5 after SCI. Conclusions The characterization of of Panx-1 expression after SCI in rats suggests that Panx-1 had a significant effect on the motor function recovery after SCI, and it was one of the important mechanisms that aggravate the secondary injury after SCI in rats. This provided experimental basis for further exploring the potential intervention target of SCI.

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