Comparison Between Separate Hydrolysis and Fermentation and Simultaneous Saccharification and Fermentation Using Dilute Acid Pretreated Lignocellulosic Biomass

2016 ◽  
pp. 3-14 ◽  
Author(s):  
Madhuri Narra ◽  
Jisha P. James ◽  
Velmurugan Balasubramanian
Keyword(s):  
Lignocellulosic Biomass ◽  
Simultaneous Saccharification And Fermentation ◽  
Dilute Acid ◽  
Separate Hydrolysis And Fermentation ◽  
Simultaneous Saccharification

Ethanol Production from Hydrothermal Pretreated Empty Fruit Bunches

2014 ◽  
Vol 917 ◽  
pp. 80-86
Author(s):  
Mohd Saman Siti Aisyah ◽  
Pacharakamol Petchpradab ◽  
Yoshimitsu Uemura ◽  
Suzana Yusup ◽  
Machi Kanna ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Lignocellulosic Biomass ◽  
Simultaneous Saccharification And Fermentation ◽  
Process Time ◽  
Empty Fruit Bunches ◽  
Separate Hydrolysis And Fermentation ◽  
Common Process ◽  
The Common ◽  
Ssf Process ◽  
Simultaneous Saccharification

Separate hydrolysis and fermentation (SHF) is the common process in producing ethanol from lignocellulosic biomass. Nowadays, simultaneous saccharification and fermentation (SSF) process has been seen as potential process for producing ethanol with shortens process time with higher yield of ethanol. Hence, in the current work, the utilization of empty fruit bunches (EFB) in SSF process was studied. In order to improve saccharification reactivity of EFB, hydrothermal pretreatment at 180 and 220 °C was used to pretreat EFB. The findings showed that SSF has the potential in producing ethanol from EFB.

PRODUCTION OF BIOFUEL FROM WASTE LIGNOCELLULOSIC BIOMASS MATERIALS BASED ON ENERGY SAVING VIEWPOINT

2012 ◽  
Vol 06 ◽  
pp. 715-720
Author(s):  
Maki Takano ◽  
Kazuhiro Hoshino
Keyword(s):  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
Steam Explosion ◽  
Simultaneous Saccharification And Fermentation ◽  
Biofuel Production ◽  
Fermentable Sugars ◽  
Renewable Material ◽  
Suitable Combination ◽  
Biomass Materials ◽  
Simultaneous Saccharification

To develop biofuel production from waste lignocellulosic biomass materials the rice straw was selected one of renewable material and the degradation condition about pretreatment and enzymatic hydrolysis to obtain effectively fermentable sugars was investigated. Rice straw was pretreated by five kinds of methods and then the components ratio of rice straw was examined. First, the steam explosion was selected based on the degradability and the requirement energy. In addition, the best suitable combination of two cellulases to effective and economical hydrolyze was determined from the degradability of these pretreated rice straws. In the simultaneous saccharification and fermentation of the steam explosion rice straw by combining cellulase cocktail and a novel fermenting fungus, 13.2 g/L ethanol was able to product for 96 h.

scholarly journals Enzymatic hydrolysis and fermentation strategies for the biorefining of pine sawdust

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7474-7491
Author(s):  
Carolina Mónica Mendieta ◽  
Fernando Esteban Felissia ◽  
Ana María Arismendy ◽  
Julia Kruyeniski ◽  
María Cristina Area
Keyword(s):  
Enzymatic Hydrolysis ◽  
Simultaneous Saccharification And Fermentation ◽  
Design Tool ◽  
Cellulolytic Enzymes ◽  
Chemical Compositions ◽  
Pine Sawdust ◽  
Second Generation Bioethanol ◽  
Separate Hydrolysis And Fermentation ◽  
Experimental Values ◽  
Simultaneous Saccharification

This work aims to evaluate second-generation bioethanol production from the soda-ethanol pulp of pine sawdust via two strategies: separate hydrolysis and fermentation and simultaneous saccharification and fermentation. A kinetics study of the enzymatic hydrolysis of separate hydrolysis and fermentation was included as a design tool. Three soda-ethanol pulps (with different chemical compositions), Cellic® Ctec2 cellulolytic enzymes, and Saccharomyces cerevisiae IMR 1181 (SC 1181) yeast were employed. The obtained kinetic parameters were as follows: an apparent constant (k) of 11.4 h-1, which represents the link frequency between cellulose and cellulase; a Michaelis-Menten apparent constant (KM) of 23.5 gL-1, that indicates the cellulose/cellulase affinity; and the apparent constant of inhibition between cellulose-glucose and cellulase (KI), which was 2.9 gL-1, 3.1 gL-1, and 6.6 gL-1 for pulps 1, 2, and 3, respectively. The kinetic model was applicable, since the calculated glucose values fit the experimental values. High bioethanol yields were obtained for pulp 3 in the separate hydrolysis and fermentation and simultaneous saccharification and fermentation processes (89.3% and 100% after 13 h and 72 h, respectively).

scholarly journals Evolutionary Engineering of Lactobacillus Bulgaricus Reduces Enzyme Usage and Enhances Conversion of Lignocellulosics to D-lactic Acid by Simultaneous Saccharification and Fermentation

2020 ◽  
Author(s):  
Vishnu Prasad J. ◽  
Tridweep K. Sahoo ◽  
Naveen S. ◽  
Guhan Jayaraman
Keyword(s):  
Lactic Acid ◽  
Lignocellulosic Biomass ◽  
Simultaneous Saccharification And Fermentation ◽  
Enzyme Hydrolysis ◽  
Lactobacillus Bulgaricus ◽  
Evolutionary Engineering ◽  
Enzyme Loading ◽  
Fermentation Processes ◽  
Platform Chemicals ◽  
Simultaneous Saccharification

Abstract BackgroundSimultaneous saccharification and fermentation (SSF) of pre-treated lignocellulosics to biofuels and other platform chemicals has long been a promising alternative to separate hydrolysis and fermentation processes. However, the disparity between the optimum conditions (temperature, pH) for fermentation and enzyme hydrolysis leads to execution of the SSF process at sub-optimal conditions, which can affect the rate of hydrolysis and cellulose conversion. The fermentation conditions could be synchronized with hydrolysis optima by carrying out the SSF at a higher temperature, but this would require a thermo-tolerant organism. Economically viable production of platform chemicals from lignocellulosic biomass has long been stymied because of the significantly higher cost of hydrolytic enzymes. The major objective of this work is to develop an SSF strategy for D- lactic acid production by a thermo-tolerant organism, in which the enzyme loading could significantly be reduced without compromising on the overall conversion. ResultsA thermo-tolerant strain of Lactobacillus bulgaricuswas developed by adaptive laboratory evolution (ALE) which enabled the SSF to be performed at 45 °C with reduced enzyme usage.Despite the reduction of enzyme loading from 15 FPU/gbiomass to 5 FPU/gbiomass, we could still achieve ~8% higher cellulose to D-LA conversion in batch SSF, in comparison to the conversion by separate enzymatic hydrolysis and fermentation processes at 45 °C and pH 5.5. Extending the batch SSF to an SSF with pulse-feeding of 5% pre-treated biomass and 5 FPU/g-biomass, at12-hour intervals (36th h – 96th h), resulted in a titer of 108 g/L D-LA and 60% conversion of cellulose to D-LA.This is one among the highest reported D-LA titers achieved from lignocellulosic biomass.ConclusionsWe have demonstrated that the SSF strategy, in conjunction with evolutionary engineering, could drastically reduce enzyme requirement and be the way forward for economical production of platform chemicals from lignocellulosics. We have shown that fed-batch SSF processes, designed with multiple pulse-feedings of the pre-treated biomass and enzyme, can be an effective way of enhancing the product concentrations.

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