Population Growths and Survival of Trichoderma Harzianum and Trichoderma Virens in Sphagnum Peat

Abstrak:Cabai merah (Capsicum annum L.) merupakan salah satu komoditas hortikultura unggulan terbesar dari golongan sayur-sayuran karena memiliki harga jual yang tinggi. Disamping itu selain harga yang tinggi, di lapangan cabai merah sering mengalami gangguan dari mulai benih disemai sampai tanaman cabai menghasilkan, hal ini disebabkan penyakit tular tanah atau pun terbawa benih. Salah satu penyakit yang memprihatinkan pada tanaman cabai merah adalah penyakit layu yang disebabkan oleh jamur Fusarium oxysporum f.sp capsici,penyakit ini sering merugikan para petani, jika tanaman sudah terserang oleh penyakitini, kerugian bisa mencapai 80 % pada tanaman cabai merah. Salah satu alternatif yang digunakan dalam Pengendalian Hama dan Penyakit terpadu (PHPT) dengan menggunakan agensia Trichoderma formulasi pelet. Penelitian ini bertujuan agar mengetahui keefektifan Trichoderma Formulasi pelet dalam menekan penyakit Layu Fusarium yang disebabkan oleh Jamur Fusarium oxysporum f.sp capsici. Penelitian ini telah dilaksanakan di laboratorium Penyakit Tumbuhan Program Studi Proteksi Tanaman dan Rumah Kasa Kebun Percobaan Fakultas Pertanian Unsyiah. Penelitian ini dimulai sejak bulan Agustus sampai Oktober 2017. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola non faktorial dengan 9 perlakuan dan 3 ulangan setiap perlakuan terdiri dari 5 unit polibag sehingga terdapat 135 unit polibag. Peubah yang diamati meliputi masa inkubasi, tinggi tanaman, jumlah daun dan persentase serangan.Hasil penelitian menunjukkan bahwa aplikasi pelet Trichoderma harzianum 2,5 g/polibagdan Trichoderma virens 2 g/polibag mampu memperlama masa inkubasi (Tidak terdapat gejala). Aplikasi pelet T. virens 1,5 g polibag dapat meningkatkan tinggi tanaman cabai merah sampai 16, 80 cm. Aplikasi pelet T. harzianum 1,5 g/polibag dapat mamacu pertumbuhan jumlah daun sebesar 9,07 helai. Aplikasi pelet T. harzianum 2,5 g/polibag dan T. virens formulasi pelet 2 g/polibag mampu menekan persentase serangan jamur Fusarium oxysporum f.sp capsici sebesar 13,68 % pada 35 hari setelah tanam.Combination of Several Doses and Species of Trichoderma Pellet Formulations In Suppressing Development Fungus of fusarium oxysporum f.sp capsici in Red Chili Breeding (Capsicum annum L.)Abstract : a red chili (Capsicum annum L.) is one of the largest superior horticultural commodity of vegetables, because it has a high selling price. Although it has high price, but in the field red chilli often disturbance from the seed sowing until the pepper plants produce, it is cause by soil contagion or even carried by seed. One of the most common diseases in red chili plants is the wilting disease cause of the fungus Fusarium oxysporum f.sp capsici. It often detrimental of the farmers if the plants have been attacked by Fusarium wilt disease, the loss could reach out about 80% in red chili plants. One of the alternative to Integrated Pest and Disease Control (PHPT) is using the Trichoderma pellet formulation agent. The reasearch purpose to determine of the effectiveness Trichoderma Formulation of pellets in suppressing Fusarium Lung disease caused by Fusarium oxysporum f.sp capsici. The research has been conducted in Plant Disease Laboratory of Plant Protection Study Program and home srceen Experimental Garden of Agriculture Faculty Unsyiah. The research was started since August to October 2017. The research uses Completely Randomized Design (RAL) non factorial pattern with 9 treatments and 3 replications each treatment consists of 5 units of polybags so that there are 135 units of polybags. The variable observed included incubation period, plant height, number of leaves and percentage of attacks. Results of the research showed that the application of Trichoderma harzianum pellet 2.5 g / polybag and Trichoderma virens 2 g / polybag was able to prolong the incubation period. Application of T. virens pellet 1.5 g polybags can increase the height of red pepper plants up to 16, 80 cm. Application of pellets T. harzianum 1.5 g / polybags can spure growth of leaves amounted to 9.07 strands. The application of T. harzianum pellet 2.5 g / polybag and T. virens pellet 2 g / polybag formulation was able to suppress the percentage of Fusarium oxysporum f.sp capsici attack by 13.68% at 35 days after planting.

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Bio-efficacy of Trichoderma species against Pigeonpea wilt pathogen

Journal of Applied and Natural Science ◽

10.31018/jans.v9i4.1531 ◽

2017 ◽

Vol 9 (4) ◽

pp. 2327-2331 ◽

Cited By ~ 1

Author(s):

Balkishan Chaudhary ◽

Sanjeev Kumar ◽

Shiva Kant Kushwaha

Keyword(s):

Volatile Compounds ◽

Trichoderma Harzianum ◽

Mycelial Growth ◽

Biocontrol Agent ◽

Trichoderma Viride ◽

Control Agent ◽

Trichoderma Virens ◽

Trichoderma Species ◽

Fusarium Udum

Three biocontrol agent viz., Trichoderma viride, Trichoderma virens and Trichoderma harzianum were evaluated to test the antagonism against Fusarium udum under in vitro conditions. All the three biocontrol agents have the potential of parasitizing the growth of Fusarium udum in vitro. The rate of parasitism was found fastest in T. viride (61.12% over growth in 96 hrs) than T. virens and T. harzianum. The volatile compounds from Trichoderma viride suppressed the mycelial growth of Fusarium udum by 43.13% and found effective when compared to Tricho-derma virens and Trichoderma harzianum. Non-volatile compounds or culture filtrate from Trichoderma virens at 15% concentration shows complete mycelial inhibition of the test fungi. The antagonist T. virens was chosen to be the most promising bio-control agent for F. udum.

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Effects of Trichoderma Species on the Growth of Fusarium Verticillioides

Bangladesh Journal of Botany ◽

10.3329/bjb.v50i2.54101 ◽

2021 ◽

Vol 50 (2) ◽

pp. 423-425

Author(s):

Ramesh Kumar ◽

Sanjeev Kumar ◽

Balkishan Chaudhary

Keyword(s):

Trichoderma Harzianum ◽

Mycelial Growth ◽

Fusarium Verticillioides ◽

Trichoderma Viride ◽

Maximum Growth ◽

Culture Technique ◽

Dual Culture ◽

Trichoderma Virens ◽

Trichoderma Species

Six biocontrol treatments viz., Trichoderma viride, Trichoderma virens, Trichoderma harzianum, T. harzianum + T. viride, T. harzianum + T. virens and T. viride + T. virens were evaluated to test the antagonism against Fusarium verticillioides under in vitro conditions. The maximum growth inhibition (90.6%) was recorded in consortium of T. harzianum + T. viride in dual culture technique . The volatile and non volatile compounds from the consortium of T. harzianum + T. viride also found best and suppressed the mycelial growth of F. verticillioides to the tune of 83.90 and 84.61 %, respectively. Bangladesh J. Bot. 50(2): 423-425, 2021 (June)

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Genomic Analysis of the Mycoparasite Pestalotiopsis sp. PG52

Polish Journal of Microbiology ◽

10.33073/pjm-2021-016 ◽

2021 ◽

Vol 70 (2) ◽

pp. 189-199

Author(s):

DENGYUN ZHANG ◽

JINDE YU ◽

CHANGLE MA ◽

LEI KONG ◽

CHENGZHONG HE ◽

...

Keyword(s):

Secondary Metabolites ◽

Plant Pathogen ◽

Trichoderma Harzianum ◽

Genomic Analysis ◽

Trichoderma Atroviride ◽

Trichoderma Virens ◽

Strong Ability

Pestalotiopsis sp. is a mycoparasite of the plant pathogen Aecidium wenshanense. To further understand the mycoparasitism mechanism of Pestalotiopsis sp., we assembled and analyzed its genome. The genome of Pestalotiopsis sp. strain PG52 was assembled into 335 scaffolds and had a size of 58.01 Mb. A total of 20,023 predicted genes and proteins were annotated. This study compared PG52 with the mycoparasites Trichoderma harzianum, Trichoderma atroviride, and Trichoderma virens. This study reveals the entirely different mycoparasitism mechanism of Pestalotiopsis compared to Trichoderma and reveals this mycoparasite’s strong ability to produce secondary metabolites.

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Aplikasi Formulasi Cair Trichoderma harzianum dan Trichoderma virens sebagai Agen Pengendali Hayati (APH) Penyakit Hawar Daun (Phytopthora palmivora) pada Bibit Kakao

Jurnal Ilmiah Mahasiswa Pertanian ◽

10.17969/jimfp.v4i2.10980 ◽

2020 ◽

Vol 4 (2) ◽

pp. 91-100

Author(s):

Desi Arida ◽

Rina Sriwati ◽

Tjut Chamzurni

Keyword(s):

Plant Height ◽

Trichoderma Harzianum ◽

Theobroma Cacao ◽

Disease Incidence ◽

Leaf Blight ◽

Phytophthora Palmivora ◽

Trichoderma Virens ◽

Liquid Formulation ◽

Trichoderma Spp ◽

Theobroma Cacao L

Abstrak. Pencegahan penyakit hawar daun oleh jamur Phytophthora palmivora pada kakao (Theobroma cacao L.) menggunakan fungisida dapat berdampak buruk terhadap lingkungan dan menimbulkan resistensi, sehingga untuk mengatasinya diperlukan alternatif lain dengan menggunakan Agen Antagonis Hayati (APH) seperti cendawan Trichoderma spp. Perbanyakan cendawan Trichoderma spp. dapat diperbanyak dengan berbagai media, salah satunya dengan formulasi cair. Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi dari T. harzianum dan T.virens dalam mengendalikan penyakit hawar daun P. palmivora pada bibit kakao. Penelitian ini dilaksanakan di Laboratorium Penyakit Tumbuhan dan Kebun Percobaan Fakultas Pertanian Universitas Syiah Kuala pada bulan Januari 2018 sampai dengan Juli 2018. Penelitian ini menggunakan Rancangan Acak Lengkap non faktorial yang terdiri dari 4 perlakuan dengan 5 ulangan, Apabila uji F menunjukkan pengaruh nyata, dilanjutkan dengan uji beda nyata terkecil pada taraf 5%. Peubah yang diamati yaitu masa inkubasi, kejadian penyakit, keparahan penyakit jumlah daun, lebar daun, diameter batang dan tinggi tanaman. Hasil penelitian ini menunjukkan aplikasi formulasi cair T. harzianum dan T. virens berpengaruh terhadap peubah masa inkubasi, kejadian penyakit, keparahan penyakit dan tinggi tanaman yaitu 18,75 cm per tanaman.Application Liquid Formulation Trichoderma harzianum and Trichoderma virens as Biological Control Agent (APH) Leaf Blight (Phytopthora palmivora) on Cocoa SeedlingsAbstract. Prevention of leaf blight by the fungus Phytophthora palmivora on cocoa (Theobroma cacao L.) using fungicides may adversely affect the environment and cause resistance, so to resolve it needed another alternative by using Biological Antagonist Agent (APH) such as Trichoderma spp. The multiplication of Trichoderma spp. can be reproduced with various media, one of them with a liquid formulation. This study aimed to determine the effect of the combination of T. harzianum and T.virens in controlling P. palmivora leaf blight in cocoa seedlings. This research was conducted at the Laboratory of Plant Disease and Experimental Garden of the Faculty of Agriculture, Syiah Kuala University in January 2018 until July 2018. This study used a non-factorial Completely Randomized Design consisting of 4 treatments with 5 replications. If the F test shows a real effect, it will continue by the smallest real difference test at the level of 5%. The variables observed were incubation period, disease incidence, disease severity, number of leaves, leaf width, stem diameter and plant height. The results of this study showed that the application of liquid formulation T. harzianum and T. virens had an effect on the incubation period, disease incidence, disease severity and plant height of 18.75 cm per plant.

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Effect of Paecilomyces lilacinus , Trichoderma harzianum and Trichoderma virens fungal extracts on the hatchability of Ancylostoma eggs

Revista Iberoamericana de Micología ◽

10.1016/j.riam.2016.04.004 ◽

2017 ◽

Vol 34 (1) ◽

pp. 28-31 ◽

Cited By ~ 2

Author(s):

Bianca Delgado Menezes Hofstätter ◽

Anelise Oliveira da Silva Fonseca ◽

Fernando de Souza Maia Filho ◽

Julia de Souza Silveira ◽

Beatriz Maroneze Persici ◽

...

Keyword(s):

Trichoderma Harzianum ◽

Paecilomyces Lilacinus ◽

Trichoderma Virens ◽

Fungal Extracts

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Effect of Exudates of Trichoderma harzianum and Trichoderma viride on the Mortality Percentage of Nymphs and Adults of Schizaphis graminum Rondani (Aphididae: Homoptera)

Basrah Journal of Agricultural Sciences ◽

10.33762/bagrs.2013.111361 ◽

2013 ◽

Vol 26 (1) ◽

pp. 372-386

Author(s):

Jinan M. Kalaf ◽

A. Benyan ◽

Layla ◽

Mustafa K. Farag

Keyword(s):

Trichoderma Harzianum ◽

Trichoderma Viride ◽

Schizaphis Graminum ◽

Mortality Percentage

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Growth of Dracaena marginata and Spathiphyllum ‘Petite’ in Sphagnum Peat- and Coconut Coir Dust-based Growing Media

Journal of Environmental Horticulture ◽

10.24266/0738-2898-17.1.49 ◽

1999 ◽

Vol 17 (1) ◽

pp. 49-52 ◽

Cited By ~ 4

Author(s):

Robert H. Stamps ◽

Michael R. Evans

Keyword(s):

Growth Parameters ◽

Cocos Nucifera ◽

Greenhouse Production ◽

Growing Media ◽

Sphagnum Peat ◽

Affected Plant ◽

Coir Dust ◽

Media Component ◽

The Media ◽

Cocos Nucifera L

Abstract A comparison was made of Canadian sphagnum peat (SP) and Philippine coconut (Cocos nucifera L.) coir dust (CD) as growing media components for greenhouse production of Dracaena marginata Bak. and Spathiphyllum Schott ‘Petite’. Three soilless foliage plant growing mixes (Cornell, Hybrid, University of Florida #2 [UF-2]) were prepared using either SP or CD and pine bark (PB), vermiculite (V), and/or perlite (P) in the following ratios (% by vol): Cornell = 50 CD or SP:25 V:25 P, Hybrid = 40 CD or SP:30 V:30 PB, UF-2 = 50 CD or SP: 50 PB. Dracaena root growth was not affected by treatments but there were significant mix × media component interactions that affected plant top growth parameters. In general, the growth and quality of D. marginata were reduced by using CD in Cornell, had no effect in Hybrid, and increased in UF-2. S. ‘Petite’ grew equally well in all growing mixes regardless of whether CD or SP was used; however, plants grew more in Cornell and Hybrid than in UF-2. S. ‘Petite’ roots, which were infested with Cylindrocladium spathiphylli, had higher grades when grown in CD than when the media contained SP.

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