Differentiation and Development of the Specialised Infection Structures Formed by Biotrophic Fungal-Plant Pathogens

1994 ◽  
pp. 171-177
Author(s):  
Jonathan R. Green ◽  
James A. Callow ◽  
Malcolm P. Leckie ◽  
Alison J. Mitchell ◽  
Richard J. O’Connell ◽  
...  
Keyword(s):  
Plant Pathogens ◽  
Infection Structures ◽  
Fungal Plant Pathogens

Infection structures of biotrophic and hemibiotrophic fungal plant pathogens

2001 ◽  
Vol 2 (2) ◽  
pp. 101-108 ◽  
Author(s):  
Sarah E. Perfect ◽  
Jonathan R. Green
Keyword(s):  
Plant Pathogens ◽  
Infection Structures ◽  
Fungal Plant Pathogens

Topographical signaling for cell differentiation in the plant pathogenic fungus, Uromyces

1989 ◽  
Vol 47 ◽  
pp. 784-785
Author(s):  
H.C. Hoch
Keyword(s):  
Plant Pathogens ◽  
Developmental Process ◽  
Pathogenic Fungus ◽  
Host Cells ◽  
Subsequent Infection ◽  
Infection Structures ◽  
Rust Diseases ◽  
Fungal Plant Pathogens ◽  
Plant Signals ◽  
Important Structure

Fungal plant pathogens invade host cells with a variety of specialized infection structures, however, for most fungi the appressorium is developmentally the first and most important structure to be formed in preparation for host colonization. It must be positioned at an appropriate site on the host in a timely way so that subsequent infection can be assured. For fungi which cause rust diseases of plants, positioning the appressorium is the most critical stage because invasion of the host can occur only via the stomata. Uredospores of these fungi (e.g.,Uromyces appendiculatus) germinate and grow, directed by the leaf (bean) surface topography toward stomata where they cease growth and develop appressoria directly over the stomatal openings. Development of the appressorium is accompanied by ameboid-like migration of the cytoplasm into the ballooning hyphal tip, DNA synthesis and nuclear division, synthesis of several “differentiation” proteins, and a rearrangement of the cytoskeleton. An orderly succession of subsequent infection structures (e.g., infection pegs, vesicles) follow in a preprogrammed sequence once the initial developmental process has been started.My research goals have been to determine what feature(s) of the host plant signals infection structure formation and how the fungus perceives these signals.

Analysis of differentiation and development of the specialized infection structures formed by biotrophic fungal plant pathogens using monoclonal antibodies

1995 ◽  
Vol 73 (S1) ◽  
pp. 408-417 ◽  
Author(s):  
Jonathan R. Green ◽  
Naomi A. Pain ◽  
Martin E. Cannell ◽  
Calum P. Leckie ◽  
Sharon McCready ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Plasma Membrane ◽  
Powdery Mildew ◽  
Plant Pathogens ◽  
Colletotrichum Lindemuthianum ◽  
Cdna Expression Library ◽  
Erysiphe Pisi ◽  
Infection Structures ◽  
Fungal Plant Pathogens

Monoclonal antibodies have been used to study the differentiation and development of the specialized infection structures formed in the Colletotrichum–bean and powdery mildew – pea interactions. In the Colletotrichum lindemuthianum – bean interaction, monoclonal antibodies have been used to show that the extracellular matrices associated with conidia, germ tubes, and appressoria differ in composition and that the extracellular glycoproteins are organized into specific regions of the fungal cell surface. Monoclonal antibody UB27 has been used to show that the plasma membrane of appressoria is differentiated into distinct domains, with the integral membrane glycoprotein identified by UB27 being excluded from the pore region. UB25 recognizes a glycoprotein located specifically in the cell wall/matrix of intracellular hyphae and is expressed only during the biotrophic phase of development. In the Erysiphe pisi – pea interaction, UB8 and UB10 identify glycoproteins specific to the haustorial plasma membrane within the haustorial complex. Monoclonal antibodies that recognize the extrahaustorial membrane have shown that this membrane contains specific components, as well as glycoproteins in common with the host plasma membrane. UB8 has been successfully used to isolate a gene sequence coding for the protein antigen, by immunoscreening a cDNA expression library prepared from infected epidermis. An antibody that recognizes the plant endoplasmic reticulum has been used to show that this structure reorganizes around the developing haustorial complex in pea epidermal cells. Key words: appressorium, biotrophy, Colletotrichum lindemuthianum, Erysiphe pisi, haustorium, monoclonal antibody, powdery mildew.

Morphological, biochemical and molecular identification of rhizobacteria isolates with potential for biocontrol of fungal plant pathogens

2021 ◽  
pp. 1-14
Author(s):  
Stephen Larbi-Koranteng ◽  
Richard Tuyee Awuah ◽  
Fredrick Kankam ◽  
Muntala Abdulai ◽  
Marian Dorcas Quain ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Plant Pathogens ◽  
Fungal Plant Pathogens

Reduction of Selenicereus stem cuttings weight by fungal plant pathogens during storage

2021 ◽  
Author(s):  
John Darby Taguiam ◽  
Edzel Evallo ◽  
Mark Angelo Balendres
Keyword(s):  
Plant Pathogens ◽  
Stem Cuttings ◽  
Fungal Plant Pathogens

scholarly journals The carbon source-dependent pattern of antimicrobial activity and gene expression in Pseudomonas donghuensis P482

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marta Matuszewska ◽  
Tomasz Maciąg ◽  
Magdalena Rajewska ◽  
Aldona Wierzbicka ◽  
Sylwia Jafra
Keyword(s):  
Gene Expression ◽  
Antimicrobial Activity ◽  
Carbon Source ◽  
Reference Genes ◽  
Plant Pathogens ◽  
Plant Protection ◽  
Carbon Sources ◽  
Unknown Compound ◽  
Fungal Plant Pathogens ◽  
The Impact

AbstractPseudomonas donghuensis P482 is a tomato rhizosphere isolate with the ability to inhibit growth of bacterial and fungal plant pathogens. Herein, we analysed the impact of the carbon source on the antibacterial activity of P482 and expression of the selected genes of three genomic regions in the P482 genome. These regions are involved in the synthesis of pyoverdine, 7-hydroxytropolone (7-HT) and an unknown compound (“cluster 17”) and are responsible for the antimicrobial activity of P482. We showed that the P482 mutants, defective in these regions, show variations and contrasting patterns of growth inhibition of the target pathogen under given nutritional conditions (with glucose or glycerol as a carbon source). We also selected and validated the reference genes for gene expression studies in P. donghuensis P482. Amongst ten candidate genes, we found gyrB, rpoD and mrdA the most stably expressed. Using selected reference genes in RT-qPCR, we assessed the expression of the genes of interest under minimal medium conditions with glucose or glycerol as carbon sources. Glycerol was shown to negatively affect the expression of genes necessary for 7-HT synthesis. The significance of this finding in the light of the role of nutrient (carbon) availability in biological plant protection is discussed.

scholarly journals Stress-Activated Protein Kinase Signalling Regulates Mycoparasitic Hyphal-Hyphal Interactions in Trichoderma atroviride

2021 ◽  
Vol 7 (5) ◽  
pp. 365
Author(s):  
Dubraska Moreno-Ruiz ◽  
Linda Salzmann ◽  
Mark D. Fricker ◽  
Susanne Zeilinger ◽  
Alexander Lichius
Keyword(s):  
Protein Kinase ◽  
Map Kinases ◽  
Plant Pathogens ◽  
Tip Growth ◽  
Mitogen Activated Protein Kinase ◽  
Trichoderma Atroviride ◽  
Contact Phase ◽  
Fungal Plant Pathogens ◽  
Signalling Network ◽  
Mapk Signalling

Trichoderma atroviride is a mycoparasitic fungus used as biological control agent against fungal plant pathogens. The recognition and appropriate morphogenetic responses to prey-derived signals are essential for successful mycoparasitism. We established microcolony confrontation assays using T. atroviride strains expressing cell division cycle 42 (Cdc42) and Ras-related C3 botulinum toxin substrate 1 (Rac1) interactive binding (CRIB) reporters to analyse morphogenetic changes and the dynamic displacement of localized GTPase activity during polarized tip growth. Microscopic analyses showed that Trichoderma experiences significant polarity stress when approaching its fungal preys. The perception of prey-derived signals is integrated via the guanosine triphosphatase (GTPase) and mitogen-activated protein kinase (MAPK) signalling network, and deletion of the MAP kinases Trichoderma MAPK 1 (Tmk1) and Tmk3 affected T. atroviride tip polarization, chemotropic growth, and contact-induced morphogenesis so severely that the establishment of mycoparasitism was highly inefficient to impossible. The responses varied depending on the prey species and the interaction stage, reflecting the high selectivity of the signalling process. Our data suggest that Tmk3 affects the polarity-stress adaptation process especially during the pre-contact phase, whereas Tmk1 regulates contact-induced morphogenesis at the early-contact phase. Neither Tmk1 nor Tmk3 loss-of-function could be fully compensated within the GTPase/MAPK signalling network underscoring the crucial importance of a sensitive polarized tip growth apparatus for successful mycoparasitism.

scholarly journals The Ustilago hordei–Barley Interaction is a Versatile System for Characterization of Fungal Effectors

2021 ◽  
Vol 7 (2) ◽  
pp. 86
Author(s):  
Bilal Ökmen ◽  
Daniela Schwammbach ◽  
Guus Bakkeren ◽  
Ulla Neumann ◽  
Gunther Doehlemann
Keyword(s):  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Expression System ◽  
Pathogenic Fungi ◽  
Effector Proteins ◽  
Mating Partner ◽  
Fungal Virulence ◽  
Functional Studies ◽  
Infection Structures ◽  
Ustilago Hordei

Obligate biotrophic fungal pathogens, such as Blumeria graminis and Puccinia graminis, are amongst the most devastating plant pathogens, causing dramatic yield losses in many economically important crops worldwide. However, a lack of reliable tools for the efficient genetic transformation has hampered studies into the molecular basis of their virulence or pathogenicity. In this study, we present the Ustilago hordei–barley pathosystem as a model to characterize effectors from different plant pathogenic fungi. We generate U. hordei solopathogenic strains, which form infectious filaments without the presence of a compatible mating partner. Solopathogenic strains are suitable for heterologous expression system for fungal virulence factors. A highly efficient Crispr/Cas9 gene editing system is made available for U. hordei. In addition, U. hordei infection structures during barley colonization are analyzed using transmission electron microscopy, showing that U. hordei forms intracellular infection structures sharing high similarity to haustoria formed by obligate rust and powdery mildew fungi. Thus, U. hordei has high potential as a fungal expression platform for functional studies of heterologous effector proteins in barley.

Metabolites of rhizobacteria antagonistic towards fungal plant pathogens

2007 ◽  
Vol 57 (1) ◽  
pp. 127-130 ◽  
Author(s):  
Garima Jha ◽  
Vanamala Anjaiah
Keyword(s):  
Plant Pathogens ◽  
Fungal Plant Pathogens
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