scholarly journals On multiple-nutrient-limited growth of microorganisms, with special reference to dual limitation by carbon and nitrogen substrates

Author(s):  
Thomas Egli
1970 ◽  
Vol 16 (8) ◽  
pp. 783-795 ◽  
Author(s):  
P. S. S. Dawson

A modification of the continuous phased culture method is described. This permits examination of changes taking place during the cell cycle (cell cycle changes) to be extended into the following period (postcycle changes).Candida utilis was grown on a glucose medium under conditions of carbon and nitrogen limitation. In nitrogen-limited growth, the size of the amino acid pool changed between the cell cycle and postcycle, but remained relatively constant for both periods in carbon-limited growth. In carbon-limited growth, the carbohydrate composition of the cells was relatively little changed, but considerable changes occurred in nitrogen-limited cells during cell cycle and postcycle periods. Changing patterns in phospholipid contents were also observed during cell cycles and postcycles of both carbon- and nitrogen-limited growths.Qualitative and quantitative changes in various cell components were related to the nutrient limiting to growth and the pattern of its use by the cells. The results illustrate the influence of environmental change upon the cell and are discussed in relation to aspects of primary and secondary metabolism in the cell.


1995 ◽  
Vol 42 (1-3) ◽  
pp. 297-314 ◽  
Author(s):  
G�tz Schroth ◽  
Dorothee Kolbe ◽  
Balle Pity ◽  
Wolfgang Zech

1999 ◽  
Vol 133 (3) ◽  
pp. 243-249 ◽  
Author(s):  
NIGEL G. HALFORD

The most important harvested organs of crop plants, such as seeds, tubers and fruits, are often described as assimilate sinks. They play little or no part in the fixation of carbon through the production of sugars through photosynthesis, or in the uptake of nitrogen and sulphur, but import these assimilated resources to support metabolism and to store them in the form of starch, oils and proteins. Wild plants store resources in seeds and tubers to later support an emergent young plant. Cultivated crops are effectively storing resources to provide us with food and many have been bred to accumulate much more than would be required otherwise. For example, approximately 80% of a cultivated potato plant's dry weight is contained in its tubers, ten times the proportion in the tubers of its wild relatives (Inoue & Tanaka 1978). Cultivation and breeding has brought about a shift in the partitioning of carbon and nitrogen assimilate between the organs of the plant.


Author(s):  
R.W. Carpenter

Interest in precipitation processes in silicon appears to be centered on transition metals (for intrinsic and extrinsic gettering), and oxygen and carbon in thermally aged materials, and on oxygen, carbon, and nitrogen in ion implanted materials to form buried dielectric layers. A steadily increasing number of applications of microanalysis to these problems are appearing. but still far less than the number of imaging/diffraction investigations. Microanalysis applications appear to be paced by instrumentation development. The precipitation reaction products are small and the presence of carbon is often an important consideration. Small high current probes are important and cryogenic specimen holders are required for consistent suppression of contamination buildup on specimen areas of interest. Focussed probes useful for microanalysis should be in the range of 0.1 to 1nA, and estimates of spatial resolution to be expected for thin foil specimens can be made from the curves shown in Fig. 1.


Sign in / Sign up

Export Citation Format

Share Document