Lipid Extraction from Plant and Muscle Tissues Using Supercritical CO2

2000 ◽  
pp. 489-498 ◽  
Author(s):  
F. Temelli
Keyword(s):  
Supercritical Co2 ◽  
Lipid Extraction ◽  
Muscle Tissues

scholarly journals Supercritical CO2 Extraction of Nannochloropsis sp.: A Lipidomic Study on the Influence of Pretreatment on Yield and Composition

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1854 ◽  
Author(s):  
Kathy Elst ◽  
Miranda Maesen ◽  
Griet Jacobs ◽  
Leen Bastiaens ◽  
Stefan Voorspoels ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Neutral Lipids ◽  
Lipid Extraction ◽  
Fatty Acid Distribution ◽  
Extraction Yield ◽  
Partial Hydrolysis ◽  
Complex Nature ◽  
Supercritical Co2 Extraction ◽  
Optimized Conditions ◽  
Chloroform Methanol

Algal lipids have gained wide interest in various applications ranging from biofuels to nutraceuticals. Given their complex nature composed of different lipid classes, a deep knowledge between extraction conditions and lipid characteristics is essential. In this paper, we investigated the influence of different pretreatments on lipid extraction with supercritical CO2 by a lipidomic approach. Pretreatment was found to double the total extraction yield, thereby reaching 23.1 wt.% comparable to the 26.9 wt.% obtained with chloroform/methanol. An increase in acylglycerides was concurrently observed, together with a nearly doubling of free fatty acids indicative of partial hydrolysis. Moreover, an alteration in the distribution of glyco- and phospholipids was noted, especially promoting digalactosyldiglycerides and phosphatidylcholine as compared to monogalactosyldiglycerides and phosphatidylglycerol. At optimized conditions, supercritical CO2 extraction provided a lipid extract richer in neutral lipids and poorer in phospholipids as compared to chloroform/methanol, though with a very similar fatty acid distribution within each lipid class.

scholarly journals Comparison of Conventional and Sustainable Lipid Extraction Methods for the Production of Oil and Protein Isolate from Edible Insect Meal

Foods ◽  
2019 ◽  
Vol 8 (11) ◽  
pp. 572 ◽  
Author(s):  
Laroche ◽  
Perreault ◽  
Marciniak ◽  
Gravel ◽  
Chamberland ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Protein Extraction ◽  
Lipid Extraction ◽  
Tenebrio Molitor ◽  
Extraction Methods ◽  
Extraction Yield ◽  
Acheta Domesticus ◽  
Human Consumption ◽  
Alternative Source ◽  
Edible Insect

Edible insects represent an interesting alternative source of protein for human consumption but the main hurdle facing the edible insect sector is low consumer acceptance. However, increased acceptance is anticipated when insects are incorporated as a processed ingredient, such as protein-rich powder, rather than presented whole. To produce edible insect fractions with high protein content, a defatting step is necessary. This study investigated the effects of six defatting methods (conventional solvents, three-phase partitioning, and supercritical CO2) on lipid extraction yield, fatty profiles, and protein extraction and purification of house cricket (Acheta domesticus) and mealworm (Tenebrio molitor) meals. Ethanol increased the lipid extraction yield (22.7%–28.8%), irrespective of the insect meal used or the extraction method applied. Supercritical CO2 gave similar lipid extraction yields as conventional methods for Tenebrio molitor (T. molitor) (22.1%) but was less efficient for Acheta domesticus (A. domesticus) (11.9%). The protein extraction yield ranged from 12.4% to 38.9% for A. domesticus, and from 11.9% to 39.3% for T. molitor, whereas purification rates ranged from 58.3% to 78.5% for A. domesticus and from 48.7% to 75.4% for T. molitor.

Comparative study of lipid extraction from microalgae by organic solvent and supercritical CO2

2011 ◽  
Vol 102 (21) ◽  
pp. 10151-10153 ◽  
Author(s):  
Chen-Hsi Cheng ◽  
Tz-Bang Du ◽  
Hsien-Chueh Pi ◽  
Shyue-Ming Jang ◽  
Yun-Huin Lin ◽  
...  
Keyword(s):  
Organic Solvent ◽  
Comparative Study ◽  
Supercritical Co2 ◽  
Lipid Extraction

Heterotrophic growth of green microalgaeDesmodesmus subspicatusin ethanol distillation wastewater (vinasse) and lipid extraction with supercritical CO2

2016 ◽  
Vol 92 (3) ◽  
pp. 573-579 ◽  
Author(s):  
Mariana Altenhofen da Silva ◽  
Gabriel Henrique Barbosa ◽  
Carolina Brito Codato ◽  
Luiz Felipe Arjonilla de Mattos ◽  
Reinaldo Gaspar Bastos ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Lipid Extraction ◽  
Heterotrophic Growth

Lipid Extraction from Olea Europea L. by Supercritical Carbon Dioxide for Analytical Use

2006 ◽  
Vol 2 (2) ◽  
Author(s):  
Giuseppe Perretti ◽  
Luigi Montanari ◽  
Paolo Fantozzi
Keyword(s):  
Carbon Dioxide ◽  
Sample Preparation ◽  
Supercritical Co2 ◽  
Oil Content ◽  
Lipid Extraction ◽  
Food Product ◽  
Product Analysis ◽  
Gravimetric Measurement ◽  
Olive Cultivar ◽  
Olea Europea

The aim of this work is to determine the feasibility of applying SFE technique by supercritical CO2, to the gravimetric measurement of lipids in olives by verifying the feasibility of extractions, studying the analytes solubility in SC-CO2, optimizing the preparation of sample, appraising the repeatability of the data obtainable with the adopted method. The olive cultivar "Moraiolo” and two different supercritical fluid laboratory instruments were used. Initial trials were performed to verify the extractability of the oil from matrix. The degree of solubility was determined by the quantitative amount of extracted and collected solute, reported for the quantity of solvent used (CO2). Once the feasibility was established and the solubility was studied, it was necessary carry out specific trials to find a better formula for the sample preparation. This involved type of matrix (humidity, structure, oil content, density, etc.) and equipment commonly available in laboratories deals in food product analysis.

scholarly journals Application of supercritical CO2 in lipid extraction – A review

2009 ◽  
Vol 95 (2) ◽  
pp. 240-253 ◽  
Author(s):  
F. Sahena ◽  
I.S.M. Zaidul ◽  
S. Jinap ◽  
A.A. Karim ◽  
K.A. Abbas ◽  
...  
Keyword(s):  
Supercritical Co2 ◽  
Lipid Extraction

A Resorcinol-Formaldehyde Resin as an Embedding Material for Electron Microscopy of Membranes

1969 ◽  
Vol 27 ◽  
pp. 328-329 ◽  
Author(s):  
J. G. Robertson ◽  
D. F. Parsons
Keyword(s):  
Electron Microscopy ◽  
Osmium Tetroxide ◽  
Neutral Lipids ◽  
Lipid Extraction ◽  
Water Soluble ◽  
Formaldehyde Resin ◽  
Electron Microscope Autoradiography ◽  
Resorcinol Formaldehyde ◽  
Major Disadvantage ◽  
Cell Organization

The extraction of lipids from tissues during fixation and embedding for electron microscopy is widely recognized as a source of possible artifact, especially at the membrane level of cell organization. Lipid extraction is also a major disadvantage in electron microscope autoradiography of radioactive lipids, as in studies of the uptake of radioactive fatty acids by intestinal slices. Retention of lipids by fixation with osmium tetroxide is generally limited to glycolipids, phospholipids and highly unsaturated neutral lipids. Saturated neutral lipids and sterols tend to be easily extracted by organic dehydrating reagents prior to embedding. Retention of the more saturated lipids in embedded tissue might be achieved by developing new cross-linking reagents, by the use of highly water soluble embedding materials or by working at very low temperatures.

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