Role of temperature on bio-printability of gelatin methacryloyl bioink in two-step cross-linking strategy for tissue engineering applications

2020 ◽  
Vol 16 (1) ◽  
pp. 015021
Author(s):  
Mohsen Janmaleki ◽  
Jun Liu ◽  
Milad Kamkar ◽  
Milad Azarmanesh ◽  
Uttandaraman Sundararaj ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cross Linking ◽  
Engineering Applications

Cellular Cross-linking of Peptide Modified Hydrogels

2004 ◽  
Vol 127 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Jeanie L. Drury ◽  
Tanyarut Boontheekul ◽  
David J. Mooney
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Density ◽  
Tensile Tests ◽  
Cross Linking ◽  
Engineering Applications ◽  
C2c12 Myoblasts ◽  
Polymer Systems ◽  
Peptide Modification ◽  
Adhesive Interactions

Peptide modification of hydrogel-forming materials is being widely explored as a means to regulate the phenotype of cells immobilized within the gels. Alternatively, we hypothesized that the adhesive interactions between cells and peptides coupled to the gel-forming materials would also enhance the overall mechanical properties of the gels. To test this hypothesis, alginate polymers were modified with RGDSP-containing peptides and the resultant polymer was used to encapsulate C2C12 myoblasts. The mechanical properties of these gels were then assessed as a function of both peptide and cell density using compression and tensile tests. Overall, it was found that above a critical peptide and cell density, encapsulated myoblasts were able to provide additional mechanical integrity to hydrogels composed of peptide-modified alginate. This occurred presumably by means of cell-peptide cross-linking of the alginate polymers, in addition to the usual Ca++ cross-linking. These results are potentially applicable to other polymer systems and important for a range of tissue engineering applications.

Cross-linking methods of electrospun fibrinogen scaffolds for tissue engineering applications

2008 ◽  
Vol 3 (4) ◽  
pp. 045001 ◽  
Author(s):  
Scott A Sell ◽  
Michael P Francis ◽  
Koyal Garg ◽  
Michael J McClure ◽  
David G Simpson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cross Linking ◽  
Engineering Applications

Chemoselective cross-linking of alginate with thiol-terminated peptides for tissue engineering applications

2012 ◽  
Vol 88 (4) ◽  
pp. 1239-1250 ◽  
Author(s):  
Silvia Bubenikova ◽  
Izabela-Cristina Stancu ◽  
Lucia Kalinovska ◽  
Etienne Schacht ◽  
Evi Lippens ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Cross Linking ◽  
Engineering Applications

A library of tunable agarose carbomer-based hydrogels for tissue engineering applications: The role of cross-linkers

2011 ◽  
Vol 123 (4) ◽  
pp. 2211-2221 ◽  
Author(s):  
Filippo Rossi ◽  
Giuseppe Perale ◽  
Giuseppe Storti ◽  
Maurizio Masi
Keyword(s):  
Tissue Engineering ◽  
Engineering Applications

scholarly journals The Adipose-Derived Stem Cell and Endothelial Cell Coculture System—Role of Growth Factors?

Cells ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 2074
Author(s):  
Dominik Steiner ◽  
Hilkea Mutschall ◽  
Sophie Winkler ◽  
Raymund E. Horch ◽  
Andreas Arkudas
Keyword(s):  
Tissue Engineering ◽  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Tissue Formation ◽  
Engineering Applications ◽  
Coculture System ◽  
Scaffold Materials ◽  
Adipose Derived Stem Cell ◽  
Bone Tissue Formation

Adequate vascularization is a fundamental prerequisite for bone regeneration, formation and tissue engineering applications. Endothelialization of scaffold materials is a promising strategy to support neovascularization and bone tissue formation. Besides oxygen and nutrition supply, the endothelial network plays an important role concerning osteogenic differentiation of osteoprogenitor cells and consecutive bone formation. In this study we aimed to enhance the growth stimulating, proangiogenic and osteogenic features of the ADSC and HUVEC coculture system by means of VEGFA165 and BMP2 application. We were able to show that sprouting phenomena and osteogenic differentiation were enhanced in the ADSC/HUVEC coculture. Furthermore, apoptosis was unidirectionally decreased in HUVECs, but these effects were not further enhanced upon VEGFA165 or BMP2 application. In summary, the ADSC/HUVEC coculture system per se is a powerful tool for bone tissue engineering applications.

Scaffolds and Tissue Engineering Applications by 3D Bio-Printing Process

2021 ◽  
pp. 718-733
Author(s):  
Ranjit Barua ◽  
Sudipto Datta ◽  
Pallab Datta ◽  
Amit Roy Chowdhury
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Manufacturing Process ◽  
Cross Linking ◽  
Engineering Applications ◽  
Printing Process ◽  
Important Thing ◽  
Bone Scaffolds ◽  
Complex Design ◽  
Different Types

3D bio-printing is a revolutionary manufacturing process that is widely used in medical fields especially in preparing bone scaffolds and tissue engineering. With the help of new biocompatible material like polymers, bio-gels, ceramics, this technology has created a new site in advanced tissue engineering and scaffolds manufacturing area. Another important thing is that, with the use of CAD file software, any complex design can be prepared (i.e., this technology does not have any limited sites). But here it is very much essential to study and analyze machine printability characteristics, cross-linking time and biocompatibility of printing objects as well as bio-ink. However, mechanical properties like shear thinning, mechanical elasticity are also required. In this chapter, different types of scaffold-preparing methods and the bio-printing process are discussed, which are used in scaffold and tissue engineering.

Human Periosteum-Derived Stem Cells for Tissue Engineering Applications: The Role of VEGF

2012 ◽  
Vol 8 (3) ◽  
pp. 882-890 ◽  
Author(s):  
C. Ferretti ◽  
V. Borsari ◽  
M. Falconi ◽  
A. Gigante ◽  
R. Lazzarini ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Engineering Applications

Electrosprayed nanoparticles and electrospun nanofibers based on natural materials: applications in tissue regeneration, drug delivery and pharmaceuticals

2015 ◽  
Vol 44 (3) ◽  
pp. 790-814 ◽  
Author(s):  
Radhakrishnan Sridhar ◽  
Rajamani Lakshminarayanan ◽  
Kalaipriya Madhaiyan ◽  
Veluchamy Amutha Barathi ◽  
Keith Hsiu Chin Lim ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Electrospun Nanofibers ◽  
Natural Polymers ◽  
Engineering Applications ◽  
Natural Materials

The role of electrospun and electrosprayed natural polymers or drug ingredients for pharmaceutical and tissue engineering applications is presented in this review.

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