Cellular Structure of Cyanidioschyzon merolae: A Minimum Set of Organelles

2017 ◽  
pp. 17-27
Author(s):  
Yuuta Imoto ◽  
Yamato Yoshida
Keyword(s):  
Cellular Structure ◽  
Cyanidioschyzon Merolae

scholarly journals CZON-cutter: a CRISPR-Cas9 system with multiplexed organelle imaging in a simple unicellular alga

2021 ◽  
Author(s):  
Yamato Yoshida ◽  
Naoto Tanaka ◽  
Yuko Mogi ◽  
Takayuki Fujiwara ◽  
Kannosuke Yabe ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cellular Structure ◽  
Fluorescent Protein ◽  
Protein Labeling ◽  
Cyanidioschyzon Merolae ◽  
Unicellular Alga ◽  
Guide Rna ◽  
Biological Features ◽  
Algal Genome ◽  
High Throughput Manner

The simple cellular structure of the unicellular alga Cyanidioschyzon merolae consists of one nucleus, one mitochondrion, one chloroplast, and one peroxisome per cell and offers unique advantages to investigate mechanisms of organellar proliferation and the cell cycle. Here, we describe an engineered clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated protein 9 (Cas9) system, CZON-cutter, for simultaneous genome editing and organellar visualization. We engineered a C. merolae strain expressing a nuclear-localized Cas9-Venus nuclease to target editing at a locus defined by a single-guide RNA (sgRNA). We then successfully edited the algal genome and visualized the mitochondrion and peroxisome in transformants by fluorescent protein reporters with different excitation wavelengths. Fluorescent protein labeling of organelles in living transformants allows validation of phenotypes associated with organellar proliferation and the cell cycle, even when the edited gene is essential. Combined with the exceptional biological features of C. merolae, CZON-cutter will be instrumental for investigating cellular and organellar division in a high-throughput manner.

scholarly journals CZON-cutter: a CRISPR-Cas9 system for multiplexed organelle imaging in a simple unicellular alga

2021 ◽  
Author(s):  
Naoto Tanaka ◽  
Yuko Mogi ◽  
Takayuki Fujiwara ◽  
Kannosuke Yabe ◽  
Yukiho Toyama ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cellular Structure ◽  
Fluorescent Protein ◽  
Protein Labeling ◽  
Cyanidioschyzon Merolae ◽  
Unicellular Alga ◽  
Guide Rna ◽  
Biological Features ◽  
Algal Genome ◽  
High Throughput Manner

The unicellular alga Cyanidioschyzon merolae has a simple cellular structure: each cell has one nucleus, one mitochondrion, one chloroplast, and one peroxisome. This simplicity offers unique advantages for investigating organellar proliferation and the cell cycle. Here, we describe CZON-cutter, an engineered clustered, regularly interspaced, short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system for simultaneous genome editing and organellar visualization. We engineered a C. merolae strain expressing a nuclear-localized Cas9-Venus nuclease for targeted editing of any locus defined by a single guide RNA (sgRNA). We then successfully edited the algal genome and visualized the mitochondrion and peroxisome in transformants using fluorescent protein reporters with different excitation wavelengths. Fluorescent protein labeling of organelles in living transformants allows us to validate phenotypes associated with organellar proliferation and the cell cycle, even when the edited gene is essential. Combined with the exceptional biological features of C. merolae, CZON-cutter will be instrumental for investigating cellular and organellar division in a high-throughput manner.

Cellular structure of lean hydrogen flames in microgravity

1990 ◽  
Author(s):  
G. PATNAIK ◽  
K. KAILASANATH
Keyword(s):  
Cellular Structure

Metallographic analysis of M2 high speed steel granules

2019 ◽  
pp. 78-85
Author(s):  
L. E. Afanasieva
Keyword(s):  
Solid Solution ◽  
Cellular Structure ◽  
High Speed ◽  
Solution Structure ◽  
Supersaturated Solid Solution ◽  
High Speed Steel ◽  
Metallographic Analysis ◽  
M2 High Speed Steel ◽  
Melt Composition

The article is devoted to the metallographic analysis of the M2 high-speed steel granules. The study is based on the investigation of the microstructure of the M2 high-speed steel granules obtained by melt atomization. It is demonstrated that granules of similar size can harden both by chemically separating and chemically non-separating mechanism. These last ones have supersaturated solid solution structure of the liquid melt composition, a dispersed dendritic-cellular structure and an increased microhardness HV = 10267±201 MPa.

scholarly journals The Influence of Pipe Bending Curvature on H2-O2 Gaseous Detonation Wave Front

2021 ◽  
Vol 11 (9) ◽  
pp. 3951
Author(s):  
Hui Zhao ◽  
Huiyuan Li ◽  
Haitao Zhao ◽  
Leisheng Li ◽  
Jian Li
Keyword(s):  
Detonation Wave ◽  
Pressure Distribution ◽  
Wave Front ◽  
Cell Size ◽  
Chemical Reaction ◽  
Cellular Structure ◽  
Gaseous Detonation ◽  
External Boundary ◽  
Propagation Characteristics ◽  
Detonation Wave Front

The influence of different bend curvatures on the detonation wave propagation was analyzed by an advanced numerical simulation system. The mechanism of propagation properties is revealed by cellular structure, internal and external boundary pressure distribution, propagation process of detonation wave and chemical reaction. The cellular structure and detonation wave front of bend with different curvature are very different. The simulation results show that the detonation wave with regular cell structure propagating through the curved parts induces detonation cell size increased by diffraction near the inner wall while detonation reflected on the bottom surface resulting in decrease of cell size. Detonation wave was affected by the rarefaction wave and compression wave in the bent pipe. The pressure distribution of the bend shows that the peak pressure in the 450 curvature is the largest, which should be paid more attention in industrial design. The chemical reaction could indicate the propagation characteristics of detonation wave, and different propagation characteristics have different profiles of chemical components.

CELLULAR STRUCTURE AND BIOLOGY OF DUPUYTREN’S DISEASE

Hand Clinics ◽  
1999 ◽  
Vol 15 (1) ◽  
pp. 21-34
Author(s):  
James J. Tomasek ◽  
Melville B. Vaughan ◽  
Carol J. Haaksma
Keyword(s):  
Cellular Structure ◽  
Dupuytren’S Disease ◽  
Dupuytren's Disease
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