Exploring Fungi-Associated Lignocellulose Degradation: Secretomic and Proteomic Approaches

2019 ◽  
pp. 251-277
Author(s):  
Akshay Shankar ◽  
Shruti Ahlawat ◽  
Krishna Kant Sharma
Keyword(s):  
Lignocellulose Degradation

Characteristics and selection of efficient lignocellulose degradation microbial community

2013 ◽  
Vol 21 (5) ◽  
pp. 621-627
Author(s):  
De-Wu WANG ◽  
Tuo YAO ◽  
Qiao-Li YANG ◽  
Guo-Tao QI ◽  
Xin-Yi LIU ◽  
...  
Keyword(s):  
Microbial Community ◽  
Lignocellulose Degradation ◽  
Selection Of

scholarly journals Microbial bioprospecting for lignocellulose degradation at a unique Greek environment

Heliyon ◽  
2021 ◽  
Vol 7 (6) ◽  
pp. e07122
Author(s):  
Daphne N. Georgiadou ◽  
Pavlos Avramidis ◽  
Efstathia Ioannou ◽  
Dimitris G. Hatzinikolaou
Keyword(s):  
Lignocellulose Degradation

scholarly journals Mechanistic strategies of microbial communities regulating lignocellulose deconstruction in a UK salt marsh

Microbiome ◽  
2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Daniel R. Leadbeater ◽  
Nicola C. Oates ◽  
Joseph P. Bennett ◽  
Yi Li ◽  
Adam A. Dowle ◽  
...  
Keyword(s):  
Salt Marsh ◽  
Salt Marshes ◽  
Molecular Mechanisms ◽  
Surface Sediments ◽  
Gene Profiling ◽  
Oxidative Enzymes ◽  
Lignocellulose Degradation ◽  
Rrna Gene ◽  
Enzymatic Mechanisms

Abstract Background Salt marshes are major natural repositories of sequestered organic carbon with high burial rates of organic matter, produced by highly productive native flora. Accumulated carbon predominantly exists as lignocellulose which is metabolised by communities of functionally diverse microbes. However, the organisms that orchestrate this process and the enzymatic mechanisms employed that regulate the accumulation, composition and permanence of this carbon stock are not yet known. We applied meta-exo-proteome proteomics and 16S rRNA gene profiling to study lignocellulose decomposition in situ within the surface level sediments of a natural established UK salt marsh. Results Our studies revealed a community dominated by Gammaproteobacteria, Bacteroidetes and Deltaproteobacteria that drive lignocellulose degradation in the salt marsh. We identify 42 families of lignocellulolytic bacteria of which the most active secretors of carbohydrate-active enzymes were observed to be Prolixibacteracea, Flavobacteriaceae, Cellvibrionaceae, Saccharospirillaceae, Alteromonadaceae, Vibrionaceae and Cytophagaceae. These families secreted lignocellulose-active glycoside hydrolase (GH) family enzymes GH3, GH5, GH6, GH9, GH10, GH11, GH13 and GH43 that were associated with degrading Spartina biomass. While fungi were present, we did not detect a lignocellulolytic contribution from fungi which are major contributors to terrestrial lignocellulose deconstruction. Oxidative enzymes such as laccases, peroxidases and lytic polysaccharide monooxygenases that are important for lignocellulose degradation in the terrestrial environment were present but not abundant, while a notable abundance of putative esterases (such as carbohydrate esterase family 1) associated with decoupling lignin from polysaccharides in lignocellulose was observed. Conclusions Here, we identify a diverse cohort of previously undefined bacteria that drive lignocellulose degradation in the surface sediments of the salt marsh environment and describe the enzymatic mechanisms they employ to facilitate this process. Our results increase the understanding of the microbial and molecular mechanisms that underpin carbon sequestration from lignocellulose within salt marsh surface sediments in situ and provide insights into the potential enzymatic mechanisms regulating the enrichment of polyphenolics in salt marsh sediments.

scholarly journals Cross-Feeding of a Toxic Metabolite in a Synthetic Lignocellulose-Degrading Microbial Community

2021 ◽  
Vol 9 (2) ◽  
pp. 321
Author(s):  
Jessica A. Lee ◽  
Alyssa C. Baugh ◽  
Nicholas J. Shevalier ◽  
Brandi Strand ◽  
Sergey Stolyar ◽  
...  
Keyword(s):  
Resource Competition ◽  
Plant Biomass ◽  
Lignocellulose Degradation ◽  
Toxic Metabolite ◽  
Growth Requirements ◽  
Mutualistic Interaction ◽  
Metabolite Exchange ◽  
Future Work ◽  
Biomass Transformation ◽  
Complex Organic

The recalcitrance of complex organic polymers such as lignocellulose is one of the major obstacles to sustainable energy production from plant biomass, and the generation of toxic intermediates can negatively impact the efficiency of microbial lignocellulose degradation. Here, we describe the development of a model microbial consortium for studying lignocellulose degradation, with the specific goal of mitigating the production of the toxin formaldehyde during the breakdown of methoxylated aromatic compounds. Included are Pseudomonas putida, a lignin degrader; Cellulomonas fimi, a cellulose degrader; and sometimes Yarrowia lipolytica, an oleaginous yeast. Unique to our system is the inclusion of Methylorubrum extorquens, a methylotroph capable of using formaldehyde for growth. We developed a defined minimal “Model Lignocellulose” growth medium for reproducible coculture experiments. We demonstrated that the formaldehyde produced by P. putida growing on vanillic acid can exceed the minimum inhibitory concentration for C. fimi, and, furthermore, that the presence of M. extorquens lowers those concentrations. We also uncovered unexpected ecological dynamics, including resource competition, and interspecies differences in growth requirements and toxin sensitivities. Finally, we introduced the possibility for a mutualistic interaction between C. fimi and M. extorquens through metabolite exchange. This study lays the foundation to enable future work incorporating metabolomic analysis and modeling, genetic engineering, and laboratory evolution, on a model system that is appropriate both for fundamental eco-evolutionary studies and for the optimization of efficiency and yield in microbially-mediated biomass transformation.

Studies on Lignocellulose Degradation by Rumen Microorganism

2013 ◽  
Vol 853 ◽  
pp. 253-259 ◽  
Author(s):  
Guan Rong Wang ◽  
Yu Lin Duan
Keyword(s):  
Cellulose Degradation ◽  
Agricultural Waste ◽  
Complex Structure ◽  
Renewable Resource ◽  
Research Progress ◽  
Lignocellulose Degradation ◽  
Enzymatic Characterization ◽  
Rumen Microorganisms ◽  
Microbial Metagenomics ◽  
Energy Processing

Lignocellulosic material is the earth's most abundant renewable resource, but because of its stable and complex structure, it is not easy for depredating and utilizing for a long time. Rumen can degrade lignocellulose, and is one of nature's most efficient fermentation fermenter; to be study rumen microorganisms has the potential to provide valuable solutions to renewable energy, processing of agricultural waste, organic waste etc. Research methods vary from screening of cellulose-degradation bacteria, enzymatic characterization, to the use of metagenomics technology to get a large number of genes directly from the rumen. This article reviews the characteristics, mechanism and contribution of different rumen microbial in degradation of lignocellulose, as well as the research progress of enzymatic characterization and rumen microbial metagenomics.

Patterns of lignocellulose degradation and secretome analysis of Trametes trogii MT

2012 ◽  
Vol 75 ◽  
pp. 55-62 ◽  
Author(s):  
Xian-Ling Ji ◽  
Wen-Ting Zhang ◽  
Ying-Ping Gai ◽  
Bao-Yun Lu ◽  
Chuan-Zhong Yuan ◽  
...  
Keyword(s):  
Lignocellulose Degradation ◽  
Secretome Analysis ◽  
Trametes Trogii

scholarly journals Boosting LPMO-driven lignocellulose degradation by polyphenol oxidase-activated lignin building blocks

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
Matthias Frommhagen ◽  
Sumanth Kumar Mutte ◽  
Adrie H. Westphal ◽  
Martijn J. Koetsier ◽  
Sandra W. A. Hinz ◽  
...  
Keyword(s):  
Polyphenol Oxidase ◽  
Building Blocks ◽  
Lignocellulose Degradation

Lignocellulose degradation by Fusarium species

1983 ◽  
Vol 61 (4) ◽  
pp. 1194-1198 ◽  
Author(s):  
John B. Sutherland ◽  
Anthony L. Pometto III ◽  
Don L. Crawford
Keyword(s):  
Wheat Straw ◽  
Degradation Products ◽  
Fusarium Species ◽  
Water Soluble ◽  
Lignocellulose Degradation ◽  
Blue Spruce ◽  
Picea Pungens ◽  
Weight Losses ◽  
Aromatic Degradation ◽  
Different Strains

Eighteen strains of fungi in the genus Fusarium, including varieties of F. episphaeria, F. lateritium, F. moniliforme, F. nivale, F. oxysporum, F. rigidiusculum, F. roseum, F. solani, and F. tricinctum, slowly degraded lignocelluloses from blue spruce (Picea pungens) and wheat (Triticum aestivum). When grown with [lignin-14C]lignocellulose from blue spruce, 15 of the Fusarium strains converted 2.2 to 4.3% of the [14C]lignin in 60 days to 14CO2 and 3.9 to 8.4% to labeled water-soluble products. When grown with unlabeled lignocellulose from wheat straw, the strains caused total weight losses in 60 days of 7 to 25%, acid-insoluble (Klason) lignin losses of 2 to 17%, and carbohydrate losses of 3 to 33%. Crude protein contents of degraded wheat-straw lignocellulose samples were 3.2 to 5.1%. Among the aromatic degradation products from wheat-straw lignocellulose degraded by different strains, as shown by gas chromatography, were p-coumaric acid, vanillic acid, vanillin, syringaldehyde, and p-hydroxybenzaldehyde.

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