Numerical Analysis of Laser-Assisted Cryopreservation of Biological Tissues

2020 ◽  
pp. 435-444
Author(s):  
Saurabh Das ◽  
Satya Prakash Kar
Keyword(s):  
Numerical Analysis ◽  
Biological Tissues

Debye Coefficients for Biological Tissues From 100 MHz to 100 GHz

2021 ◽  
Vol 35 (11) ◽  
pp. 1424-1425
Author(s):  
Rachel Lumnitzer ◽  
Allison Tanner ◽  
Atef Elsherbeni
Keyword(s):  
Numerical Analysis ◽  
Human Body ◽  
Complex Permittivity ◽  
Electromagnetic Waves ◽  
Fdtd Method ◽  
Biological Tissues ◽  
Expansion Coefficients ◽  
Numerical Solver

Debye modeling of dispersive, biological tissues allows for the numerical analysis of electromagnetic waves in the vicinity of a human body using the FDTD method. Three-term Debye expansion coefficients for 55 human biological tissues are determined via a two-step numerical solver. The Debye coefficients obtained predict precisely the complex permittivity of the experimentally available tissue data.

NUMERICAL ANALYSIS OF THE FOOT IN HEALTHY AND DEGENERATIVE CONDITIONS

2017 ◽  
Vol 17 (06) ◽  
pp. 1750095
Author(s):  
CHIARA GIULIA FONTANELLA ◽  
ARTURO NICOLA NATALI ◽  
EMANUELE LUIGI CARNIEL
Keyword(s):  
Numerical Analysis ◽  
Soft Tissue ◽  
Mechanical Response ◽  
Biological Tissues ◽  
Tissue Mechanics ◽  
Specific Loading ◽  
Constitutive Parameters ◽  
Plantar Soft Tissue

The aim of this work is the development of a 3D numerical model of the foot that allows evaluating the influence of degenerative phenomena on the foot mechanical functionality. Such degenerative phenomena induce histo-morphological alterations and significant modification of the plantar soft tissue mechanical properties, as stiffening and lower damping capabilities. The finite element model of the foot is developed starting from the analysis of biomedical images. Different constitutive models define the mechanical response of the biological tissues. Because of the major role of plantar soft tissue in the here proposed analysis, a specific visco-hyperelastic constitutive formulation is provided considering the typical features of the tissue mechanics, as geometric and material non linearity, almost incompressible behavior and time-dependent phenomena. Constitutive parameters are identified by the analysis of experimental data from in vitro and in vivo mechanical tests, leading to the identification of a range of constitutive parameters for healthy and degenerative conditions. Numerical analyses are developed to investigate the influence of the progression of the degeneration on the distribution of stress and of strain within foot tissues during static standing. Numerical results show the increase of stress values with the appearance of degenerative conditions, showing the typical stiffening phenomenon. The mechanical response of the plantar soft tissue during specific loading condition and the influence of degenerative phenomena on foot mechanics can be evaluated with numerical analysis.

Three-Dimensional Visualization of the T-System of Frog Muscle Using High Voltage Electron Microscopy and a Lanthanum Stain

1977 ◽  
Vol 35 ◽  
pp. 570-571 ◽  
Author(s):  
Lee D. Peachey ◽  
Clara Franzini-Armstrong
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Three Dimensional ◽  
Biological Tissues ◽  
High Voltage Electron Microscopy ◽  
Transverse Tubular System ◽  
Peroxidase Reaction ◽  
Voltage Electron ◽  
High Voltage Electron ◽  
T System

The effective study of biological tissues in thick slices of embedded material by high voltage electron microscopy (HVEM) requires highly selective staining of those structures to be visualized so that they are not hidden or obscured by other structures in the image. A tilt pair of micrographs with subsequent stereoscopic viewing can be an important aid in three-dimensional visualization of these images, once an appropriate stain has been found. The peroxidase reaction has been used for this purpose in visualizing the T-system (transverse tubular system) of frog skeletal muscle by HVEM (1). We have found infiltration with lanthanum hydroxide to be particularly useful for three-dimensional visualization of certain aspects of the structure of the T- system in skeletal muscles of the frog. Specifically, lanthanum more completely fills the lumen of the tubules and is denser than the peroxidase reaction product.

On the High Voltage Electron Microscopy (1 MeV) of Biological Thick Sections

1972 ◽  
Vol 30 ◽  
pp. 464-465
Author(s):  
William H. Massover
Keyword(s):  
Electron Microscopy ◽  
High Voltage ◽  
Present Report ◽  
Biological Tissues ◽  
Specimen Preparation ◽  
Technical Problems ◽  
High Voltage Electron Microscopy ◽  
Thin Sections ◽  
Voltage Electron ◽  
High Voltage Electron

Stereoscopic examination of thick sections of fixed and embedded biological tissues by high voltage electron microscopy has been shown to allow direct visualization of three-dimensional fine structure. The present report will consider the occurrence of some new technical problems in specimen preparation and Image interpretation that are not common during lower voltage studies of thin sections.Thick Sectioning and Tissue Coloration - Epon sections of 0.5 μm or more that are cut with glass knives do not have a uniform thickness as Judged by their interference colors; these colors change with time during their flotation on the knife bath, and again when drying onto the specimen support. Quoted thicknesses thus must be considered only as rough estimates unless measured in specific regions by other methods. Chloroform vapors do not always result in good spreading of thick sections; however, they will spread spontaneously to large degrees after resting on the flotation bath for several minutes. Ribbons of thick sections have been almost impossible to obtain.

Rapid Critical Point Drying of Tissues in a Parr Bomb

1972 ◽  
Vol 30 ◽  
pp. 400-401
Author(s):  
C.A. Baechler ◽  
W. C. Pitchford ◽  
J. M. Riddle ◽  
C.B. Boyd ◽  
H. Kanagawa ◽  
...  
Keyword(s):  
Critical Point ◽  
Critical Pressure ◽  
Soft Tissues ◽  
Biological Tissues ◽  
Critical Temperatures ◽  
Air Drying ◽  
The Past ◽  
Critical Point Drying ◽  
Great Stress ◽  
Infiltration Techniques

Preservation of the topographic ultrastructure of soft biological tissues for examination by scanning electron microscopy has been accomplished in the past by using lengthy epoxy infiltration techniques, or dehydration in ethanol or acetone followed by air drying. Since the former technique requires several days of preparation and the latter technique subjects the tissues to great stress during the phase change encountered during air-drying, an alternate rapid, economical, and reliable method of surface structure preservation was developed. Turnbill and Philpott had used a fluorocarbon for the critical point drying of soft tissues and indicated the advantages of working with fluids having both moderately low critical pressures as well as low critical temperatures. Freon-116 (duPont) which has a critical temperature of 19. 7 C and a critical pressure of 432 psi was used in this study.

Applications of Time-OF-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)

1990 ◽  
Vol 48 (2) ◽  
pp. 308-309
Author(s):  
Bruno Schueler ◽  
Robert W. Odom
Keyword(s):  
Mass Spectrometry ◽  
Secondary Ion Mass Spectrometry ◽  
Structural Information ◽  
Time Of Flight ◽  
Biological Tissues ◽  
Polymer Surfaces ◽  
Tof Sims ◽  
Secondary Ions ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Time-of-flight secondary ion mass spectrometry (TOF-SIMS) provides unique capabilities for elemental and molecular compositional analysis of a wide variety of surfaces. This relatively new technique is finding increasing applications in analyses concerned with determining the chemical composition of various polymer surfaces, identifying the composition of organic and inorganic residues on surfaces and the localization of molecular or structurally significant secondary ions signals from biological tissues. TOF-SIMS analyses are typically performed under low primary ion dose (static SIMS) conditions and hence the secondary ions formed often contain significant structural information.This paper will present an overview of current TOF-SIMS instrumentation with particular emphasis on the stigmatic imaging ion microscope developed in the authors’ laboratory. This discussion will be followed by a presentation of several useful applications of the technique for the characterization of polymer surfaces and biological tissues specimens. Particular attention in these applications will focus on how the analytical problem impacts the performance requirements of the mass spectrometer and vice-versa.

Sign in / Sign up

Export Citation Format

Share Document