Bacterial Cellulose Based Hydrogel Film for Sustainable Food Packaging

AbstractThis study aimed to obtain possible materials for future antimicrobial food packaging applications based on biodegradable bacterial cellulose (BC). BC is a fermentation product obtained by Gluconacetobacter xylinum using food or agricultural wastes as substrate. In this work we investigated the synergistic effect of zinc oxide nanoparticles (ZnO NPs) and propolis extracts deposited on BC. ZnO NPs were generated in the presence of ultrasounds directly on the surface of BC films. The BC-ZnO composites were further impregnated with ethanolic propolis extracts (EEP) with different concentrations.The composition of raw propolis and EEP were previously determined by gas-chromatography mass-spectrometry (GC-MS), while the antioxidant activity was evaluated by TEAC (Trolox equivalent antioxidant capacity). The analysis methods performed on BC-ZnO composites such as scanning electron microscopy (SEM), thermo-gravimetrically analysis (TGA), and energy-dispersive X-ray spectroscopy (EDX) proved that ZnO NPs were formed and embedded in the whole structure of BC films. The BC-ZnO-propolis films were characterized by SEM and X-ray photon spectroscopy (XPS) in order to investigate the surface modifications. The antimicrobial synergistic effect of the BC-ZnO-propolis films were evaluated against Escherichia coli, Bacillus subtilis, and Candida albicans. The experimental results revealed that BC-ZnO had no influence on Gram-negative and eukaryotic cells.

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Antibacterial activity in gelatin-bacterial cellulose composite film by thermally crosslinking with cinnamaldehyde towards food packaging application

Food Packaging and Shelf Life ◽

10.1016/j.fpsl.2021.100766 ◽

2022 ◽

Vol 31 ◽

pp. 100766

Author(s):

Nutchanat Thongsrikhem ◽

Siriporn Taokaew ◽

Malinee Sriariyanun ◽

Suchata Kirdponpattara

Keyword(s):

Antibacterial Activity ◽

Composite Film ◽

Bacterial Cellulose ◽

Food Packaging ◽

Cellulose Composite

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Curcumin entrapped gelatin/ionically modified bacterial cellulose based self-healable hydrogel film: An eco-friendly sustainable synthesis method of wound healing patch

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2018.10.196 ◽

2019 ◽

Vol 122 ◽

pp. 940-953 ◽

Cited By ~ 21

Author(s):

Moumita Khamrai ◽

Sovan Lal Banerjee ◽

Saikat Paul ◽

Sarthik Samanta ◽

Patit Paban Kundu

Keyword(s):

Wound Healing ◽

Bacterial Cellulose ◽

Synthesis Method ◽

Hydrogel Film

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Gamma Ray-Induced Synthesis of Silver Nanoparticles using Bacterial Cellulose as a Multi-Functional Agent and its Application

10.21203/rs.3.rs-522859/v1 ◽

2021 ◽

Author(s):

Nasser H. Mohammad ◽

gamal Mohamed elsherbiny ◽

Ali A. Hammad ◽

Ahmed A. Askar ◽

Salwa A. Abou El Nour

Keyword(s):

Antibacterial Activity ◽

Bacterial Cellulose ◽

Food Packaging ◽

Gamma Ray ◽

Color Change ◽

Spherical Shape ◽

Inhibition Zone ◽

Food Samples ◽

One Step ◽

Novel Method

Abstract Antibacterial coatings based on bacterial cellulose (BC) have been widely used in many fields including food packaging and wound dressing. In this study, we aimed to synthesis of colloidal AgNPs and BC/ AgNP composite by using BC as a reducing and capping agent in one step reaction induced by gamma-ray. Bacterial strain Komagataeibacter rhaeticus N1 MW322708 was used for biosynthesis BC by inoculation on Hestrin and Schramm medium and incubated statically at 35 °C for 10 days. BC sheet was formed, harvested, purified, and dried, then used for the synthesis of AgNPs and BC/AgNP by soaked 0.05 g of dried BC in 10ml of 1mM aqueous AgNO3 solution for 2h and then irradiated by gamma-ray under different doses. Color change from yellow to deep brown indicated the synthesis of AgNPs and BC/AgNP. The optical spectra of synthesized AgNPs revealed that the surface plasmon resonance was localized around 420 nm. DLS analysis showed that the mean diameter of AgNPs was 49.5 nm with a -19.36-mV value of zeta potential. TEM images revealed the spherical shape of synthesized AgNPs. The results of FESEM, FTIR, and XRD confirmed the formation of BC/AgNO3 composite. The highly crystalline nature of the BC membrane and BC/AgNP composite was observed in XRD measurements with a crystal size of 5.416 and 5.409 nm, respectively. The antibacterial activity of BC and BC/AgNP against pathogenic bacterial isolated from Pastirma food samples revealed that BC does not show antibacterial activity, while BC/AgNP composite showed antibacterial potency against Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, Proteus mirabilis, and Escherichia coli, with an inhibition zone of (mm) 9±1, 9±0.57, 10±1.15, 8±0.5 and 7±0.28, respectively. We concluded that this novel method presented in this paper offers a promising route for both AgNPs and BC/AgNP composites synthesis using a green, renewable biopolymer as a multifunctional agent and potential to be applied in the future development of food packing, biomedical instruments, and therapeutics.

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Natural paper-layered composites with air barrier properties achieved by coating with bacterial cellulose

BioResources ◽

10.15376/biores.15.4.9569-9574 ◽

2020 ◽

Vol 15 (4) ◽

pp. 9569-9574

Author(s):

Marta Kaźmierczak ◽

Tomasz P. Olejnik ◽

Magdalena Kmiotek

Keyword(s):

Bacterial Cellulose ◽

Food Packaging ◽

Barrier Properties ◽

Cellulose Fibers ◽

Packaging Material ◽

Layered Composites ◽

Chemical Additives ◽

Cellulosic Fibers ◽

Biodegradable Packaging ◽

Cellulose Pulp

In some respects the safest food packaging material is paper that is completely free of chemical additives, made only from primary cellulosic fibers. There is no information in the literature on giving paper barrier properties using nanocellulose without any additives, especially bacterial cellulose, by applying a coating to a fibrous semi-product. In order to prepare paper-layered composites, paper sheets made of beaten or non-beaten softwood or hardwood cellulose pulp, or their 50/50 (wt./wt.) mix, were used in the experiment. After the application of bacterial cellulose onto the sheets, the paper became completely impermeable to air, which means that fine microbial fibers had filled the voids (pores) between plant cellulose fibers. The results of the experiment could be regarded as a perfect, biodegradable packaging material.

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