Potential of gamma radiation enhancing the biosynthesis of tropane alkaloids in black henbane (Hyoscyamus niger L.)

Euphytica ◽  
1989 ◽  
Vol 40 (3) ◽  
pp. 253-258
Author(s):  
J. R. Sharma ◽  
R. K. Lal ◽  
H. O. Misra ◽  
M. M. Gupta ◽  
R. S. Ram
Keyword(s):  
Gamma Radiation ◽  
Tropane Alkaloids ◽  
Hyoscyamus Niger ◽  
Black Henbane

scholarly journals Production of tropane alkaloids in Hyoscyamus niger (black henbane) hairy roots grown in bubble-column and spray bioreactors

2013 ◽  
Vol 36 (4) ◽  
pp. 843-853 ◽  
Author(s):  
Zbigniew Jaremicz ◽  
Maria Luczkiewicz ◽  
Adam Kokotkiewicz ◽  
Aleksandra Krolicka ◽  
Pawel Sowinski
Keyword(s):  
Hairy Roots ◽  
Bubble Column ◽  
Tropane Alkaloids ◽  
Hyoscyamus Niger ◽  
Black Henbane

Introduced North American Black Henbane (Hyoscyamus niger) Populations are Biennial

2014 ◽  
Vol 7 (4) ◽  
pp. 624-630 ◽  
Author(s):  
Christa E. Fettig ◽  
Ruth A. Hufbauer
Keyword(s):  
North American ◽  
Common Garden ◽  
Hyoscyamus Niger ◽  
Active Growth ◽  
Black Henbane ◽  
Introduced Populations ◽  
Winter Temperatures ◽  
American Black ◽  
The Relationship ◽  
Bolting And Flowering

AbstractBlack henbane can be either annual or biennial. We investigated which life cycle is found in four introduced western North American populations. Plants were grown in a greenhouse common garden until half were vernalized by exposure to natural winter temperatures, while the other half remained in the greenhouse above 20 C, with 16 h of light and 8 h of dark. In total the plants were monitored 313 d after germination. We measured whether plants bolted, the time it took for bolting to commence, and the size at bolting. All vernalized plants bolted after 117 d of active growth (within 26 d of the end of the vernalization treatment), whereas only 26% of the nonvernalized plants bolted after an average of 278 d of active growth. Vernalized plants bolted at a smaller size than the nonvernalized plants that bolted (28 vs. 41 leaves on average). In the nonvernalized plants, the relationship between time to bolting and size was strong, but not so with the vernalized plants. Our results indicate that introduced black henbane plants are biennial, and that vernalization is more critical to bolting and flowering than reaching a certain size. Nonetheless, the fact that nonvernalized plants were capable of bolting if grown long enough suggests that vernalization is not the only cue that can trigger reproduction in introduced populations.

Induced polygenic changes occurring simultaneously with major gene changes in black henbane (Hyoscyamus niger L.)

1992 ◽  
Vol 85 (4) ◽  
pp. 445-450 ◽  
Author(s):  
J. R. Sharma ◽  
R. K. Lal ◽  
H. O. Mishra ◽  
S. Sharma
Keyword(s):  
Major Gene ◽  
Hyoscyamus Niger ◽  
Black Henbane

scholarly journals Inorganic ions in the medium modify tropane alkaloids and riboflavin output in Hyoscyamus niger root cultures

2012 ◽  
Vol 8 (29) ◽  
pp. 73 ◽  
Author(s):  
Vallo Matto ◽  
Elmar Arak ◽  
Ain Raal ◽  
Tõnis Vardja ◽  
Rael Vardja ◽  
...  
Keyword(s):  
Inorganic Ions ◽  
Tropane Alkaloids ◽  
Root Cultures ◽  
Hyoscyamus Niger

scholarly journals Influence of gibberellins on flower formation in Hyoscyamus niger L.

2014 ◽  
Vol 52 (1) ◽  
pp. 71-75
Author(s):  
Jan Kopcewicz ◽  
Gabriela Centkowska
Keyword(s):  
Flower Formation ◽  
Axillary Meristem ◽  
Light Conditions ◽  
Hyoscyamus Niger ◽  
Black Henbane ◽  
Partial Differentiation ◽  
Long Day ◽  
Full Development

Gibberellins (GA<sub>4+7</sub>) and gibberellin-like substances isolated from generatively induced black henbane (<em>Hyoscyamus niger</> L.) bring about the growth of shoots and a partial differentiation of axillary meristem in black henbane plants grown under non-inductive light conditions. Long-lasting application of gibberellins, however, did not result in full development of flowers in the majority of the plants investigated. Thus, it seems, that gibberellins are not specific flowering hormones in black henbane - a long-day plant.

scholarly journals Effects of gamma irradiation on the alkaloid content in seeds of Datura stramonium and the radiosensitivity of derived seedlings

2019 ◽  
Vol 6 (4) ◽  
pp. 533-540
Author(s):  
Nabila Benslimani ◽  
Madjda Khelifi-Slaoui ◽  
Abdelkader Morsli ◽  
Amar Djerrad ◽  
Ezz Al-Dein Al-Ramamneh ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Gamma Rays ◽  
Damage Index ◽  
Tropane Alkaloids ◽  
Datura Stramonium ◽  
Hyoscyamus Niger ◽  
Morphological Variations ◽  
Solanaceae Family

Tropane alkaloids are a group of secondary metabolites occurring naturally in Solanaceae family as Atropa belladona, Datura stramonium, Mandragora officinalis, and Hyoscyamus niger. These molecules have valuable therapeutic applications, for example, atropine and hyoscyamine are utilized as antimuscarinic besides being stomach and intestinal diseases drugs. Plants of the Solanaceae family can provide a natural yet less expensive source of these compounds. Hitherto, in order to emphasize these metabolites biosynthesis, D. stramonium seeds were irradiated using a cobalt-60 source of gamma rays of 5 to 80 Gy and germinated in vitro on MS medium in growth controlled chamber. Mutagenesis of D. stramonium seeds was attempted aiming at obtaining plants from in vitro source that are genetically variable for enhancing the biosynthesis of secondary metabolites, namely alkaloids. Results indicated that D. stramonium seeds exhibited a good radiosensitivity and the mutagen damage index GR (30-50) for D. stramonium was determined at 80 Gy. The Characterization of alkaloids (Atropine and hyoscyamine) was done by infrared spectroscopy which showed that alkaloids content of the irradiated seeds is altered by irradiation as the reference bands were not found with all doses used. In addition, seedlings grown from irradiated in vitro seeds exhibited remarkable morphological variations that varied based on the employed dose of gamma rays. These findings permitted the selection of the optimal irradiation dose (80 Gy) to induce mutations that are likely to prompt changes at genetic and metabolic level of the targeted alkaloids.

scholarly journals The Effect of Simulated Microgravity On The Antioxidant Capacity And Alkaloid Formation In The Hyoscyamus Niger

2021 ◽  
Author(s):  
Roghayeh Pourhabibian ◽  
Alireza Iranbakhsh ◽  
Mostafa Ebadi ◽  
Halimeh Hassanpour ◽  
Azadeh Hekmat
Keyword(s):  
Gene Expression ◽  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Simulated Microgravity ◽  
Tropane Alkaloids ◽  
Mitogen Activated Protein Kinase ◽  
Hyoscyamus Niger ◽  
Pal Activity

Abstract Microgravity is one of the most important abiotic stresses in space. In the case of plant exposure to short term microgravity, plants establish strategies to response to these stresses and promote growth and survival. We hypothesized that the simulated microgravity can promote the antioxidant capacity and the formation of secondary metabolites such as tropane alkaloids in the Hyoscyamus niger. Callus induction was conducted by putting hypocotyl segments of H. niger seedlings in solid MS medium supplemented with 1 mg L−1 2,4-D and 1 mg L−1 BAP growth regulators. Then, the sub-cultured calli were placed on a clinostat for 3, 7 and 10 days. We performed Atropine and Scopolamine determination through HPLC. PAL (Phenyle alanine amonalyase) and antioxidant activity were also determined. Gene expression analysis of jasmonic acid (JA), Hyoscyamine 6-beta Hydroxylase (H6H), Putrescine N-methyltransferase (PMT), mitogen-activated protein kinase (MAPK) and ethylene responsive element binding (EREB) was performed using quantitative real time PCR. Findings showed that microgravity had a positive effect on the antioxidant capacity, Atropine and Scopolamine production in the H. niger calli. However, microgravity had a negative effect on the PAL activity. Furthermore, gene expression analysis indicated that microgravity significantly induced gene expression of the H6H, PMT and JA. It was also revealed that callus growth, carbohydrate and protein content increased in response to microgravity treatment. We conclude that microgravity can be considered as a potent factor to induce plant antioxidant activity and tropane alkaloids formation to be applicable in the pharmaceutical and medicinal industries.

Hyoscyamine and scopolamine production of black henbane (Hyoscyamus niger) infected with Pseudomonas putida and P. fluorescens strains under water deficit stress

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
M Ghorbanpour ◽  
N Majnoon Hosseini ◽  
S Rezazadeh ◽  
M Omidi ◽  
K Khavazi ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Water Deficit ◽  
Water Deficit Stress ◽  
Hyoscyamus Niger ◽  
Black Henbane ◽  
Hyoscyamine And Scopolamine

Physical and physiological dormancy in black henbane (Hyoscyamus niger L.) seeds

2004 ◽  
Vol 47 (4) ◽  
pp. 391-395 ◽  
Author(s):  
Cüneyt çrak ◽  
Kudret Kevseroglu ◽  
Birsen Sağlam
Keyword(s):  
Hyoscyamus Niger ◽  
Black Henbane ◽  
Physiological Dormancy

Tropane alkaloids production in transgenic Hyoscyamus niger hairy root cultures over-expressing Putrescine N-methyltransferase is methyl jasmonate-dependent

Planta ◽  
2006 ◽  
Vol 225 (4) ◽  
pp. 887-896 ◽  
Author(s):  
Lei Zhang ◽  
Bin Yang ◽  
Beibei Lu ◽  
Guoyin Kai ◽  
Zinan Wang ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Root ◽  
Tropane Alkaloids ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Hyoscyamus Niger
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