Quality evaluation of brine shrimp Artemia cysts produced in Asian salt ponds

An immunodetection assay on Western blots has been used to determine the thiol proteinase content and composition in cysts from 12 populations of the brine shrimp Artemia. Our results showed no differences in the subunit composition of the thiol proteinase among cysts from eight bisexual strains and four parthenogenic strains, and confirmed an earlier finding that the proteinase is composed of two subunits of 25.9 and 31.5 kilodaltons. In contrast, we found that Artemia cysts from parthenogenic strains contain 17.1 ng/cyst of the thiol proteinase, while cysts from bisexual strains contain 8.2 ng/cyst of the thiol proteinase. Also, there was a good linear correlation (r = 0.863; p < 0.001) between the thiol proteinase content and cyst mass. Embryo fractionation experiments showed that 82% of the thiol proteinase was in the cytosol, while 14 and 4%, respectively, were in the nuclei/yolk platelets and mitochondria/lysosome fractions. Measurements of the thiol proteinase content of developing Artemia embryos showed that the proteinase content was relatively constant during early development, suggesting that the activity of the thiol proteinase gene(s) may be constitutive and not developmentally regulated in Artemia embryos.Key words: Artemia, proteinase, development, cathepsin B, Western blotting.

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THE CAPABILITY OF BRINE SHRIMP TEST AS A TERATOGENICITY SCREENING SYSTEM

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i3.16511 ◽

2017 ◽

Vol 10 (3) ◽

pp. 454

Author(s):

Meti Widiya Lestari ◽

Andreanus A Soemardji ◽

Irda Fidrianny ◽

Ayda T Yusuf

Keyword(s):

Valproic Acid ◽

Body Length ◽

Screening Test ◽

Brine Shrimp ◽

Chemical Characteristic ◽

Test Substance ◽

Screening System ◽

Significant Difference ◽

Artemia Cysts ◽

Seawater Medium

ABSTRACTObjectives: The goals of this study were to analyze the capability of brine shrimp test (BST) as a potent teratogenicity screening system on teratogenicagents (methotrexate, captopril, diclofenac, phenytoin, warfarin, and valproic acid).Methods: Artemia cysts were hatched into 1st stage nauplii, then taken and put into seawater medium which contain test substance and kept alive until2nd stage, 3rd stage, and 4th stage, and number of deaths, morphological abnormalities, body length, and retarded of development were observed for each stage.Results: Hatch ability of cysts in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml, diclofenac 0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid2.5 mg/ml were significantly different compared to control (p<0.05). Nauplii survival in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml, diclofenac0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid 2.5 mg/ml were significantly different to control (p<0.05). The morphological abnormalitieswas found in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml. Nauplii with retarded development were expressed in methotrexate 0.015 mg/ml,captopril 0.25 mg/ml, diclofenac 0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid 2.5 mg/ml. Significant difference in body length was presentedin captopril 0.25 mg/ml, and phenytoin 1.56 mg/ml compared to control (p<0.05).Conclusion: BST can be used as an alternative method of the teratogenic screening test, although not as sensitive teratogenic tests on mammals. Thisscreening method was not suitable for a compound which its chemical characteristic can change the tonicity of the medium.Keywords: Brine shrimp test, Teratogenicity, Methotrexate, Captopril, Diclofenac, Phenytoin, Warfarin, Valproic acid.

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BRINE SHRIMP TERATOGENIC SCREENING TEST ON SOME SELECTED PLANTS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i8.18861 ◽

2017 ◽

Vol 10 (8) ◽

pp. 96

Author(s):

Meti Widiya Lestari ◽

Irda Fidrianny ◽

Ayda T Yusuf ◽

Andreanus A Soemardji

Keyword(s):

Body Length ◽

Screening Test ◽

Brine Shrimp ◽

Punica Granatum ◽

Test Substance ◽

Foeniculum Vulgare ◽

Morphological Abnormalities ◽

Third Stage ◽

Elephantopus Scaber ◽

Artemia Cysts

Objectives: The aims of this research were to analyze teratogenic properties on some selected plants which often used for woman’s health were pomegranate (Punica granatum L.) pericarp, coriander (Coriandrum sativum L.) seeds, liman (Elephantopus scaber L.) leaves, fennel (Foeniculum vulgare M.) seeds, and kaempferia (Kaempferia galanga L.) rhizomes using brine shrimp teratogenic screening test (BSTST) method.Methods: Artemia cysts were hatched into first stage nauplii then taken and put into seawater medium which contain test substance and kept alive until the second stage, third stage, and fourth stage and then observed number of deaths, morphological abnormalities, body length, and retarded of development for each stage.Results: Hatchability of cysts in fennel seeds compared extracts 1 and 0.5 mg/ml, coriander seeds extract 1 mg/ml, pomegranate rinds extract 6 mg/ml, and liman leaves extracts 6, 3, and 1.5 mg/ml were significantly different to control (p<0.05). Survival nauplii in fennel seeds extracts 1 and 0.5 mg/ml and liman leaves extracts 6 and 3 mg/ml were significantly different to control (p<0.05). The morphological abnormalities were exposed on coriander seeds extract 1 mg/ml, liman leaves extract 6 mg/ml, and pomegranate rinds extract 3 mg/ml. Nauplii with retarded development were presented on fennel seeds extracts 1 and 0.5 mg/ml, coriander seeds extracts 1 and 0.25 mg/ml, pomegranate rinds extracts 6, 3, and 1.5 mg/ml, and liman leaves extracts 3 and 1.5 mg/ml. Nauplii body length on plants extract showed no significantly difference compared to control.Conclusion: Based on BSTST, fennel seed and pomegranate rind extracts had no teratogenic effect, kaempferia rhizomes act as larvicide its analog in mamalia as embryo lethal, while coriander seeds and liman leaves extracts potentially had teratogenic properties.

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Variability of size characteristics and survival of the nauplii of Crimean brine shrimp Artemia spp. (Branchiopoda: Anostraca) feeding on different species of microalgae

Marine Biological Journal ◽

10.21072/mbj.2019.04.1.08 ◽

2019 ◽

Vol 4 (1) ◽

pp. 91-99

Author(s):

D. Yu. Smirnov ◽

L. O. Aganesova ◽

A. N. Khanaychenko

Keyword(s):

Marine Fish ◽

Brine Shrimp ◽

Fish Larvae ◽

Average Length ◽

Isochrysis Galbana ◽

Average Diameter ◽

Live Food ◽

Essential Components ◽

Size Growth ◽

Artemia Cysts

Nauplii of brine shrimps Artemia spp. (Branchiopod: Anostraca) are one of the main species of live food used in marine fish larviculture. Specialized formulated enrichments or microalgae containing essential components for fish larvae are routinely used for improvement of biochemical composition of Artemia. Size, growth rate and survival of nauplii and metanauplii are important when Artemia is used as a live food. The goal of this study was to carry out comparative analysis of size characteristics and survival of metanauplii of the Crimean brine shrimp (produced by “Artemia cysts” company) feeding on different species of microalgae. Nauplii were hatched in accordance with the generally accepted procedure. Microalgae of different taxons – Isochrysis galbana, Prorocentrum micans, Gymnodinium wulffii, Prorocentrum cordatum, Tetraselmis suecica, Phaeodactylum tricornutum – were used for feeding metanauplii. The average diameter of dry Artemia cysts was 0.230 mm. The average length and width of nauplii were 0.473 and 0.150 mm, respectively. The average length of 1-day old metanauplii fed on T. suesica [(0,698 ± 0,014) mm] was significantly less than that of 1-day metanauplii fed on I. galbana, P. micans, G. wulfii, and Ph. tricornutum (P < 0.05). The average length and width of the 2-day and 3-days old metanauplii fed on I. galbana (1.19 and 0.324; 1.53 and 0.47 mm, respectively) were significantly larger than those of metanauplii fed on Ph. tricornutum, T. suesica, P. micans, and P. cordatum. Metanauplii fed on P. cordatum were significantly smaller than those fed on other diets. The survival of metanauplii fed on P. cordatum, P. micans, and T. suecica was the highest (above 95 %). The combination of the smallest sizes and highest survival rate of metanauplii fed on P. cordatum (microalgae with high DHA/EPA content) assumes the use on metanauplii as an experimental live food for marine fish larvae.

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Intracellular water in Artemia cysts (brine shrimp)

Biophysical Journal ◽

10.1016/s0006-3495(90)82393-6 ◽

1990 ◽

Vol 58 (2) ◽

pp. 483-491 ◽

Cited By ~ 5

Author(s):

S.R. Kasturi ◽

P.K. Seitz ◽

D.C. Chang ◽

C.F. Hazlewood

Keyword(s):

Brine Shrimp ◽

Intracellular Water ◽

Artemia Cysts

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Predator–prey interactions between native brine shrimp Artemia parthenogenetica and the alien boatman Trichocorixa verticalis: influence of salinity, predator sex, and size, abundance and parasitic status of prey

PeerJ ◽

10.7717/peerj.3554 ◽

2017 ◽

Vol 5 ◽

pp. e3554 ◽

Cited By ~ 4

Author(s):

Vanessa Céspedes ◽

Marta I. Sánchez ◽

Andy J. Green

Keyword(s):

Brine Shrimp ◽

Life Stage ◽

Predation Rate ◽

Salt Ponds ◽

Predator Prey ◽

Caribbean Islands ◽

Artemia Parthenogenetica ◽

Introduced Range ◽

Cladoceran Zooplankton ◽

Predation Rates

Trichocorixa verticalis (T. verticalis), native to North America and the Caribbean islands, is an invasive waterboatman species (Corixidae) in the southwest of the Iberian Peninsula. Previous studies in the native range have suggested that predation by T. verticalis can regulate the abundance of Anostracan and Cladoceran zooplankton in saline ecosystems, causing increases in phytoplankton through a trophic cascade. In this experimental study, we tested the predator–prey relationship between the native brine shrimp Artemia parthenogenetica, and T. verticalis from the Odiel salt ponds in SW Spain. In three experiments, we investigated (1) the effects of Artemia life stage (metanauplii, juveniles, and adults), (2) abundance (three, six, and 12 adult Artemia) and (3) parasitic status (Artemia infected with avian cestodes or uninfected) on predation rates by T. verticalis. We also considered how predation rates in all three experiments were influenced by the sex of T. verticalis and by different salinities (25 and 55 g l−1). Experiment 1 showed that predation rates were highest for metanauplii, possibly because their photophilic behavior makes them more prone to predation. In Experiment 2, we found that predation rate was higher for female T. verticalis and the higher salinity, although the strength of the sex effect varied between treatments. Experiment 3 showed that T. verticalis selectively predated adult Artemia infected with cestodes (red in color), as previously reported for predation by avian final hosts. Collectively, these results indicate that T. verticalis are important predators in their introduced range, and are likely to reduce the abundance of Artemia in more salt ponds as they expand their range, thus increasing phytoplankton abundance through trophic cascades.

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