Effects of immobilization on biomass production and acetic acid fermentation of Acetobacter aceti as a function of temperature and pH

1996 ◽  
Vol 18 (4) ◽  
pp. 393-396 ◽  
Author(s):  
Judit Krisch ◽  
B�la Szaj�ni
Keyword(s):  
Acetic Acid ◽  
Biomass Production ◽  
Acetobacter Aceti ◽  
Acid Fermentation ◽  
Acetic Acid Fermentation

scholarly journals Analysis of the basic components and free amino acid composition of pineapple fruit vinegar

2020 ◽  
Vol 185 ◽  
pp. 04047
Author(s):  
Xiao-fang Wang ◽  
Yuhao Hu ◽  
Ji-Hua Li ◽  
Li Zhang ◽  
Xiao Gong ◽  
...  
Keyword(s):  
Amino Acids ◽  
Acetic Acid ◽  
Amino Acid ◽  
Free Amino Acids ◽  
Free Amino ◽  
Acetobacter Aceti ◽  
Acid Fermentation ◽  
Acetic Acid Fermentation ◽  
Acetic Fermentation ◽  
Pineapple Fruit

Acetic acid fermentation is an essential step in producing high-quality vinegar. In this study, the alcoholic medium was used as a seed broth for acetic fermentation using Acetobacter aceti as the inoculum for approximately 7 days at 32℃ to obtain 45.87g/L acetic acid. During the Acetic acid fermentation stage, the content of the total polyphenols decreased first and then increased. Based on amino acid analyzer analysis, pineapple vinegar contains 18 kinds of free amino acids. And the contents of sweet and umami free amino acids are the main free amino acids, followed by bitter amino acids.

scholarly journals Kajian Metode dan Waktu Fermentasi Cairan Pulpa pada Perubahan Karakteristik Cuka Kakao

2017 ◽  
Vol 37 (1) ◽  
pp. 39
Author(s):  
G.P. Ganda Putra ◽  
Ni Made Wartini ◽  
Luh Putu Trisna Darmayanti
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Acetic Acid ◽  
Raw Material ◽  
Acetobacter Aceti ◽  
Acid Fermentation ◽  
Fermentation Time ◽  
Natural Fermentation ◽  
Acetic Acid Fermentation ◽  
Fermentation Method ◽  
Sugar Addition

The pulp water as byproduct of cocoa beans fermentation is  potential to be used as a raw material for making cocoa vinegar,  but unfortunately the content of acetic acid is relatively low. So  that, it is necessary to increase the content of acetic acid, by  performed further fermentation method with several addition of  carbon sources (sugar and alcohol) and fermentation time. The  purposes of this research were: (1) to study the effect of the  addition of sugar, alcohol and fermentation time on different  urther fermentation method on the characteristics of cocoa vinegar  and (2) to determine the optimal further fermentation  method and fermentation time for the production of cocoa vinegar  with the highest content of acetic acid. Several  fermentation methods were conducted by using three different  methods e.g.the alcoholic and acetic acid fermentation (2 stages),  acetic acid fermentation (1 stage), and fermentation  without inoculum (natural). The experimental design of this study was using a factorial BRD two factors on the 2 stages and the 1 stage fermentation method, as well as the simple RBD on the  natural fermentation. The first factor on the 2 stages fermentation methods used two different inoculum e.g. Saccharomyces  cerevisiae and Acetobacter aceti, 4 different concentration of sugar (4, 6, 8 and 10 %), while on the 1 stage fermentation method  used inoculum Acetobacter aceti, is 4 different concentrations of  alcohol addition (6, 8, 10, and 12 %), while the second factor is  the same, namely 6 levels of fermentation time (0, 5, 10, 15, 20,  and 25 days). Meanwhile, the treatment of fermentation time  on the natural fermentation method was the same as the  second factor. All methods of fermentation were performed at  room temperature in 2 blocks/replications.The results showed  that: 1) the characteristics of cocoa vinegar and OD660 of watery  pulp were affected by : (1) the treatment of the sugar addition  and fermentation time and their interaction on the 2 stages  fermentation method by the treatment of the alcohol addition and  fermentation time, their interaction on the 1 stage  fermentation method, and by the treatment of fermentation time  on the natural fermentation method, and (2) the cocoa vinegar  with the highest content of acetic acid was produced on the 2  stages fermentation method by the addition of 6 % sugar within  25 days (2.35 %), on the 1 stage fermentation method by the   ddition of 10 % alcohol within 20 days (3.37 %), and on the  natural fermentation method within 15 days (2.65 %),  respectively. Our result showed that the 1 stage fermentation  method with the addition of 10 % alcohol using inoculum of  Acetobacter aceti within 20 days is the most optimal further  fermentation method for the production of cocoa vinegar.ABSTRAKCairan pulpa hasil samping fermentasi biji kakao berpotensi  sebagai bahan baku pembuatan cuka kakao, tetapi kadar asam asetat yang dihasilkan relatif rendah. Untuk itu, perlu dilakukan  upaya peningkatan kadar asam asetat antara lain dengan melakukan beberapa metode fermentasi lanjutan dengan variasi penambahan sumber karbon (gula dan alkohol) dan lama fermentasi. Tujuan penelitian ini adalah: (1) mengkaji pengaruh  penambahan gula, alkohol dan lama fermentasi pada metode  fermentasi lanjutan yang berbeda terhadap karakteristik cuka  kakao dan (2) menetapkan metode fermentasi lanjutan dan  lama fermentasi yang optimal untuk produksi cuka kakao dengan kadar asam asetat tertinggi. Metode fermentasi lanjutan yang  dilakukan terdiri dari 3 metode yaitu: fermentasi alkohol dan  asam asetat (2 tahap), fermentasi asam asetat (1 tahap), dan  fermentasi tanpa inokulum (alami). Rancangan percobaan pada  penelitian ini menggunakan Rancangan Acak Kelompok (RAK)  faktorial 2 faktor pada metode fermentasi 2 tahap dan 1 tahap, serta RAK faktor tunggal pada fermentasi alami. Faktor I pada  metode fermentasi 2 tahap yang menggunakan inokulum Saccharomyces cerevisiae dan Acetobacter aceti, adalah  penambahan gula 4 konsentrasi (4, 6, 8, dan 10 %), dan pada metode fermentasi 1 tahap, yang menggunakan inokulum  Acetobacter aceti, adalah penambahan alkohol 4 konsentrasi (6,  8, 10, dan 12 %), sedangkan faktor II lama fermentasi (0, 5, 10,  15, 20, dan 25 hari). Sementara itu perlakuan lama fermentasi pada metode fermentasi alami sama seperti pada  faktor II. Semua metode fermentasi dilakukan pada suhu kamar  dalam 2 kelompok. Hasil penelitian menunjukkan bahwa: (1)  karakteristik cuka kakao dan OD660 cairan pulpa dipengaruhi  oleh perlakuan penambahan gula dan lama fermentasi serta  interaksinya pada metode fermentasi 2 tahap dan oleh perlakuan penambahan alkohol dan lama fermentasi serta  interaksinya pada fermentasi 1 tahap, serta oleh perlakuan lama  fermentasi alami, dan (2) cuka kakao dengan kadar asam asetat  tertinggi masing-masing dihasilkan pada fermentasi 2  tahap dengan penambahan gula cenderung 6 % dalam waktu 25 hari (2,35 %), fermentasi 1 tahap dengan penambahan alkohol 10 % dalam waktu 20 hari (3,37 %), dan fermentasi alami dalam  waktu 15 hari (2,65 %). Dengan demikian metode fermentasi 1  tahap dengan penambahan alkohol 10 % menggunakan  inokulum Acetobacter aceti dalam waktu 20 hari merupakan metode fermentasi lanjutan yang paling optimal untuk produksi cuka kakao.

Monitoring the microbial community during solid-state acetic acid fermentation of Zhenjiang aromatic vinegar

2011 ◽  
Vol 28 (6) ◽  
pp. 1175-1181 ◽  
Author(s):  
Wei Xu ◽  
Zhiyong Huang ◽  
Xiaojun Zhang ◽  
Qi Li ◽  
Zhenming Lu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Microbial Community ◽  
Solid State ◽  
Acid Fermentation ◽  
Acetic Acid Fermentation

Pomegranate juice functional constituents after alcoholic and acetic acid fermentation

2014 ◽  
Vol 8 ◽  
pp. 161-168 ◽  
Author(s):  
Stella A. Ordoudi ◽  
Fani Mantzouridou ◽  
Eleni Daftsiou ◽  
Christine Malo ◽  
Efimia Hatzidimitriou ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Pomegranate Juice ◽  
Acid Fermentation ◽  
Acetic Acid Fermentation

Improvement of the Flavor and Quality of Watermelon Vinegar by High Ethanol Fermentation using Ethanol-Tolerant Acetic Acid Bacteria

2017 ◽  
Vol 13 (4) ◽  
Author(s):  
Yang Chen ◽  
Ye Bai ◽  
Dongsheng Li ◽  
Chao Wang ◽  
Ning Xu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Ethanol Fermentation ◽  
Cell Mass ◽  
Acetic Acid Bacteria ◽  
Acid Fermentation ◽  
Acetic Acid Fermentation ◽  
Essential Step ◽  
High Ethanol ◽  
Acceptance Score

Abstract Acetic acid fermentation is an essential step in the production of high-quality fruit vinegar and typically involves the use of acetic acid bacteria (AAB). The present study showed that the high cell mass and acetic acid yields of ethanol-tolerant AAB under high ethanol conditions were related to the high activities and stability of both pyrroloquinoline quinone (PQQ)-dependent alcohol dehydrogenase and PQQ-dependent aldehyde dehydrogenase. Additionally, in acetic acid fermentation with watermelon wine (8 % ethanol), the main organic acids (acetic, tartaric and malic acids) produced by ethanol-tolerant Acetobacter pasteurianus AAB4 were higher than those by conventional A. pasteurianus AS1.41 (1.42-fold, 3.53-fold and 2.12-fold, respectively). Also, the main esters (ethyl acetate and phenylethyl acetate) produced by AAB4 were higher than those by AS1.41 (1.69-fold and 1.48-fold, respectively). In addition, the total sweet and umami free amino acids produced by AAB4 increased significantly. According to sensory analysis, the flavor, taste and overall acceptance score of watermelon vinegar produced by AAB4 were significantly higher than those by AS1.41. Therefore, high ethanol fermentation with ethanol-tolerant AAB improved the flavor and quality of watermelon vinegar, indicating that this technology can be applied to fruit vinegar production.

Modeling and Optimization of Acetic Acid Fermentation

2019 ◽  
pp. 299-325
Author(s):  
Isidoro García-García ◽  
Jorge E. Jiménez-Hornero ◽  
Inés María Santos-Dueñas ◽  
Zoilo González-Granados ◽  
Ana María Cañete-Rodríguez
Keyword(s):  
Acetic Acid ◽  
Acid Fermentation ◽  
Modeling And Optimization ◽  
Acetic Acid Fermentation
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