scholarly journals Immunohistochemical identification of factor X-like antigen in the A cells of the normal human pancreas

Diabetologia ◽  
1982 ◽  
Vol 23 (3) ◽  
pp. 255-260 ◽  
Author(s):  
C. Betterle ◽  
A. Trevisan ◽  
A. Girolami
Keyword(s):  
Human Pancreas ◽  
Factor X ◽  
A Cells ◽  
Normal Human ◽  
Immunohistochemical Identification

A Chromogenic Factor X Assay With New Standardized Reagents

1979 ◽  
Author(s):  
P Friberger ◽  
C Lenne
Keyword(s):  
Human Plasma ◽  
Suitable Method ◽  
Chromogenic Substrate ◽  
Factor X ◽  
Normal Human Plasma ◽  
Normal Plasma ◽  
Normal Human

A recently published method for Factor X (FX) assay (1) utilizing Russel's Viper Venom (RVV) and a chromogenic substrate has been further investigated by testing a large number of parameters. This method has been considered as a suitable method for monitoring coumarol treatment (Bergström et al).The conditions for the activation of FX by purified preparations of the RVV have been studied as well as the conditions for FXa determination with a new chromogenic substrate Bz-Ile-Glu(γ-piperidyl)-Gly-Arg-pNA (S-2337). Both purified factors and normal plasma have been used. The effect of plasma inhibitors as well as the selectivity of the method has been studied.The reproducibility and stability of the different reagents and standards have been studied and found to be good.The amount of FXa activity obtained from normal human plasma has been titrated with FXa inhibitors of known purity.1) Aurell L. et al, Thromb. Res., 11, 595 (1977)2) Bergström et al, Thromb. Res., 12, 531 (1978)

A Hitherto Undescribed Naturally Occurring Plasma Antagonist of Activated Factor X Inhibitor (Antithrombin III)

1979 ◽  
Author(s):  
E. T. Yin ◽  
W. J. Salsgiver ◽  
O. Tangen
Keyword(s):  
Equilibrium State ◽  
Human Plasma ◽  
Antithrombin Iii ◽  
Circumstantial Evidence ◽  
Incubation Mixture ◽  
Factor X ◽  
Normal Human Plasma ◽  
Plasma Component ◽  
Naturally Occurring ◽  
Normal Human

Circumstantial evidence suggested that normal human plasma contained a substance regulating the neutralization of F.Xa by F.Xa inhibitor(XaI), (Yin et.al.,Adv.Exper. Med. & Biol., 52 : 239, 1975, Plenum Press, N.Y.).This plasma component has now been isolated and partially purified in our laboratory, and tentatively designated as “Anti-XaI”.In experiments employing purified components, when Anti-XaI was incubated at 37°C with F.Xa, Xal and heparin for two minutes at pH7.5, the amount of F.Xa inhibited was inversely proportional to the Anti-XaI concentration. But, when the F.Xa was replaced by thrombin in the incubation mixture, the neutralization of thrombin clotting activity was undisturbed.Anti-XaI was found to be neither PF3 nor PF4.These and other data strongly suggest that the “Antithrombin III pathway” is more complex than currently believed to be. In circulating blood an equilibrium state must exist between Anti-XaI and XaI.Under certain conditions when the Anti-XaI activity is predominant the rate of F.Xa neutralization bv XaI then becomes slower than the activation of prothrombin to thrombin by F.Xa.

IMMUNOLOGICAL REACTIVITY OF INSULIN IN HUMAN SERUM

1966 ◽  
Vol 53 (4) ◽  
pp. 673-680 ◽  
Author(s):  
Torsten Deckert ◽  
Kai R. Jorgensen
Keyword(s):  
Normal Subjects ◽  
The Other ◽  
Human Pancreas ◽  
Porcine Pancreas ◽  
Crystalline Insulin ◽  
Endogenous Insulin ◽  
Significant Difference ◽  
Human Sera ◽  
Normal Human ◽  
The One

ABSTRACT The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales & Randle's (1963) immunoassay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pig and ox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal subjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non diabetic subjects and crystalline pig insulin.

Expression and distribution of the Na+- HCO 3 − cotransporter in human pancreas

1999 ◽  
Vol 277 (2) ◽  
pp. G487-G494 ◽  
Author(s):  
Christopher R. Marino ◽  
Virginia Jeanes ◽  
Walter F. Boron ◽  
Bernhard M. Schmitt
Keyword(s):  
Northern Blot ◽  
Islet Cells ◽  
Rat Kidney ◽  
Physiological Role ◽  
Human Pancreas ◽  
Transmembrane Conductance Regulator ◽  
Transmembrane Conductance ◽  
Cellular Mechanisms ◽  
Double Labeling ◽  
Normal Human

The cellular mechanisms of [Formula: see text] secretion in the human pancreas are unclear. Expression of a Na+-[Formula: see text]cotransporter (NBC) mRNA has been observed recently, but the distribution and physiological role of the NBC protein are not known. Here we examined the expression and localization of NBC in human pancreas by Northern blot, immunoblot, and immunofluorescence microscopy. Rat kidney NBC probes detected a single 9.5-kb band by Northern blot. On immunoblots, two polyclonal antisera directed against different epitopes of rat kidney NBC identified a single ∼130-kDa protein. In cryosections of normal human pancreas, both antisera labeled basolateral membranes of large, morphologically identifiable ducts and produced a distinct labeling pattern in the remainder of the parenchyma. In double-labeling experiments, NBC immunoreactivity in the parenchyma colocalized with the Na+-K+pump, a basolateral marker. In contrast, NBC and cystic fibrosis transmembrane conductance regulator, an apical membrane marker, were detected within the same histological structures but at different subcellular localizations. The NBC antisera did not label acinar or islet cells. Our observations suggest that secretion of[Formula: see text] by human pancreatic duct cells involves the basolateral uptake of Na+and[Formula: see text] via NBC, an electrogenic Na+-[Formula: see text]cotransporter.

Immunohistochemical identification of the pacemaker cajal cells in the normal human vagina

2005 ◽  
Vol 272 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Ahmed Shafik ◽  
Olfat El-Sibai ◽  
Ismail Shafik ◽  
Ali A. Shafik
Keyword(s):  
Cajal Cells ◽  
Human Vagina ◽  
Normal Human ◽  
Immunohistochemical Identification

Insulin and C-peptide co-localization in theβ granules of normal human pancreas and insulinomas

1989 ◽  
Vol 416 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Moshe Kalina ◽  
Lars Grimelius ◽  
Bjorn Cedermark ◽  
Ilan Hammel
Keyword(s):  
Human Pancreas ◽  
C Peptide ◽  
Normal Human

CD44 in normal human pancreas and pancreatic carcinoma cell lines

2000 ◽  
Vol 21 (1) ◽  
pp. 97-106 ◽  
Author(s):  
J�rg Ringel ◽  
Ralf Jesnowski ◽  
Christian Schmidt ◽  
Jens Ringel ◽  
Hans J. K�hler ◽  
...  
Keyword(s):  
Pancreatic Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Human Pancreas ◽  
Carcinoma Cell Lines ◽  
Normal Human

Neuronal plasticity in chronic pancreatitis is mediated via the neurturin/GFRα2 axis

2012 ◽  
Vol 303 (9) ◽  
pp. G1017-G1028 ◽  
Author(s):  
Ihsan Ekin Demir ◽  
Kun Wang ◽  
Elke Tieftrunk ◽  
Nathalia A. Giese ◽  
Baocai Xing ◽  
...  
Keyword(s):  
Chronic Pancreatitis ◽  
Glial Cell ◽  
Neurotrophic Factor ◽  
Human Pancreas ◽  
Parasympathetic Innervation ◽  
Tissue Extracts ◽  
Normal Human ◽  
The Impact

The glial cell line-derived neurotrophic factor (GDNF) family member neurturin (NRTN) and its receptor GFRα2 play a deciding role in the normal development of pancreatic parasympathetic innervation. In this study, we aimed at investigating the role of NRTN/GFRα2 axis in pancreatic neuropathy in human chronic pancreatitis (CP). Expression of NRTN/GFRα2 was compared between normal human pancreas (NP) and CP tissues via immunohistochemistry, immunoblotting, and quantitative RT-PCR and correlated to abdominal pain sensation. To elucidate the impact of NRTN in pancreatic neuroplasticity, neuronal phenotype and glial density were quantified via an in vitro neuroplasticity assay in dissociated newborn rat dorsal root ganglia (DRG) cultured 1) in CP tissue extracts depleted from NRTN, 2) in NP, 3) in untreated CP tissue extracts, and 4) CP extracts in which nerve growth factor, glial cell derived-neurotrophic factor, or TGF-β1was depleted. NRTN and GFRα2 were highly upregulated in CP, especially in intrapancreatic nerves and the extracellular matrix. CP tissue demonstrated increased amounts of mature multimeric NRTN and elevated levels of GFRα2. The noticeable neurotrophic effect of CP tissue extracts on DRG neurons was diminished upon blockade of NRTN from these extracts. However, blockade of NRTN from CP extracts did not influence the density of DRG glia cells. In conclusion, the NRTN/GFRα2 axis is activated during the course of CP and represents a major key player in the reactive neural alterations in CP. This is the first study to provide functional evidence for the contribution of neurotrophic factors to neuroplasticity in CP.

Hypoxia activates pancreatic stellate cells: Development of an organotypic culture model of thick slices of normal human pancreas

Pancreatology ◽  
2012 ◽  
Vol 12 (6) ◽  
pp. 588
Author(s):  
V. Rebours ◽  
M. Albuquerque ◽  
P. Ruszniewski ◽  
A. Sauvanet ◽  
V. Paradis ◽  
...  
Keyword(s):  
Organotypic Culture ◽  
Stellate Cells ◽  
Pancreatic Stellate Cells ◽  
Human Pancreas ◽  
Culture Model ◽  
Normal Human
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